US5102783A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Composition and method for culturing and freezing cells and tissues
Assignee: PARTICIPATION HOLDINGS Ltd
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Filing: 1990-01-12
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Granted: 1992-04-07
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Expires: 2010-01-12
Abstract
Abstract The present invention includes a composition and method for culturing and freezing cells and tissues without serum or serum products in the culture medium by substituting hyaluronic acid for the serum or serum products. The composition and method are especially well-suited for culturing and freezing embryos, ova and sperm. The present invention reduces the risk of transmittal of microorganisms such as viruses.
Inventors
- 👤 Stanley J. Alkemade
- 👤 Andre Palasz
- 👤 Reuben J. Mapletoft
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 09:02
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125; A61K35/54
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
50 words
A composition for freezing cells or tissues comprising a solution containing the nutritional substances necessary to support the cells or tissues and hyaluronic acid having an average molecular weight of not less than approximately 2×10 5 Daltons in a concentration effective to be a substitute for serum or serum products.
Claim 2
Dependent Claim
50 words
A composition for freezing cells or tissues comprising a solution containing the nutritional substances necessary to support the cells or tissues and hyaluronic acid having an average molecular weight of not less than approximately 2×10 5 Daltons in a concentration effective to be a substitute for serum or serum products.
Claim 3
Dependent Claim
24 words
The serum-free medium of claim 1, wherein the hyaluronic acid has an average molecular weight of between approximately 5×10 5 to 1.2×10 6 Daltons.
Claim 4
Dependent Claim
20 words
The serum-free medium of claim 1, wherein the hyaluronic acid has an average molecular weight of approximately 7.5×10 5 Daltons.
Claim 5
Dependent Claim
11 words
The serum-free medium of claim 1, wherein the cells are embryos.
Claim 6
Dependent Claim
12 words
The serum-free medium of claim 1, wherein the cells are unfertilized ova.
Claim 7
Dependent Claim
11 words
The serum-free medium of claim 1 wherein the cells are sperm.
Claim 8
Dependent Claim
16 words
The serum-free medium of claim 1, wherein the medium further comprises an effective amount of surfactant.
Claim 9
Dependent Claim
12 words
The serum-free medium of claim 7, wherein the surfactant is poloxamer 188.
Claim 10
Independent Claim
84 words
A method for freezing animal cells or tissues comprising the steps of: a. mixing animal cells or tissues with a medium, the medium comprising a solution containing the nutritional substances necessary to support the cells or tissues and hyaluronic acid having an average molecular weight of more than 2×10 5 Daltons in a concentration effective to be a substitute for serum or serum products; and b. lowering the temperature of the medium sufficiently to preserve the animal cells or tissues in a viable condition.
Claim 11
Independent Claim
84 words
A method for freezing animal cells or tissues comprising the steps of: a. mixing animal cells or tissues with a medium, the medium comprising a solution containing the nutritional substances necessary to support the cells or tissues and hyaluronic acid having an average molecular weight of more than 2×10 5 Daltons in a concentration effective to be a substitute for serum or serum products; and b. lowering the temperature of the medium sufficiently to preserve the animal cells or tissues in a viable condition.
Claim 12
Dependent Claim
23 words
The method of claim 9, wherein the hyaluronic acid has an average molecular weight of between approximately 5×10 5 to 1.2×10 6 Daltons.
Claim 13
Dependent Claim
19 words
The method of claim 9, wherein the hyaluronic acid has an average molecular weight of approximately 7.5×10 5 Daltons.
Claim 14
Dependent Claim
10 words
The method of claim 9, wherein the cells are embryos.
Claim 15
Dependent Claim
11 words
The method of claim 9, wherein the cells are unfertilized ova.
Claim 16
Dependent Claim
10 words
The method of claim 9, wherein the cells are sperm.
Claim 17
Dependent Claim
15 words
The method of claim 9, wherein the medium further comprises an effective amount of surfactant.
Claim 18
Dependent Claim
11 words
The method of claim 15 wherein the surfactant is poloxamer 188.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-04-07 | 1995-10-07 | 1996-04-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-04-07 | 1999-10-07 | 2000-04-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-04-07 | 2003-10-07 | 2004-04-07 | $7700.00 | Window Closed / Lapsed |