US5098833A
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DNA sequence encoding a functional domain of a lymphocyte homing receptor
Assignee: Genentech Inc
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Filing: 1989-02-23
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Granted: 1992-03-24
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Expires: 2009-02-23
Abstract
Abstract DNA isolates coding for a lymphocyte homing receptor and methods of obtaining such DNA are provided, together with expression systems for recombinant production of the lymphocyte homing receptor useful in therapeutic or diagnostic compositions.
Inventors
- 👤 Laurence A. Lasky
- 👤 Steven D. Rosen
- 👤 Scott E. Stachel
- 👤 Mark S. Singer
- 👤 Ted A. Yednock
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 05:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/32; C07K2319/70; C07K2319/705
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
53 words
A DNA isolate able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity.
Claim 2
Dependent Claim
53 words
A DNA isolate able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity.
Claim 3
Dependent Claim
26 words
The DNA isolate of claim 1 wherein the DNA isolate is free of genomic DNA which encodes another polypeptide from the source of the DNA isolate.
Claim 4
Dependent Claim
21 words
The DNA isolate of claim 1, wherein the DNA encodes a polypeptide having the amino acid sequence shown in FIG. 1.
Claim 5
Dependent Claim
21 words
The DNA isolate of claim 1, wherein the DNA encodes a polypeptide having the amino acid sequence shown in FIG. 2.
Claim 6
Dependent Claim
24 words
The DNA isolate of claim 1, comprising DNA encoding an LHR carbohydrate binding domain free of epidermal growth factor domains and complement binding domains.
Claim 7
Dependent Claim
25 words
The DNA isolate of claim 1, comprising DNA encoding an LHR epidermal growth factor binding domain free of carbohydrate binding domains and complement binding domains.
Claim 8
Dependent Claim
25 words
The DNA isolate of claim 1, comprising DNA encoding an LHR complement binding domain free of carbohydrate binding domains and epidermal growth factor binding domains.
Claim 9
Dependent Claim
20 words
The DNA isolate of claim 1, comprising DNA encoding an LHR complement binding domain and an epidermal growth factor domain.
Claim 10
Dependent Claim
44 words
The DNA isolate of claim 1, having DNA encoding a LHR carbohydrate binding domain, a LHR epidermal growth factor binding domain, and a LHR complement binding domain, wherein the DNA encoding the LHR carbohydrate binding domain is replaced by a heterologous carbohydrate binding domain.
Claim 11
Dependent Claim
48 words
The DNA isolate of claim 11, having DNA encoding a LHR carbohydrate binding domain, a LHR epidermal growth factor binding domain, and a LHR complement binding domain, wherein the DNA encoding the LHR epidermal growth factor binding domain is replaced by a heterologous epidermal growth factor binding domain.
Claim 12
Dependent Claim
44 words
The DNA isolate of claim 1, having DNA encoding a LHR carbohydrate binding domain, a LHR epidermal growth factor binding domain, and a LHR complement binding domain, wherein the DNA encoding the LHR complement binding domain is replaced by a heterologous complement binding domain.
Claim 13
Dependent Claim
58 words
A recombinant expression vector comprising a DNA sequence able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity.
Claim 14
Dependent Claim
58 words
A recombinant expression vector comprising a DNA sequence able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity.
Claim 15
Dependent Claim
14 words
A composition comprising a cell transformed with the recombinant expression vector of claim 12.
Claim 16
Dependent Claim
12 words
The composition of claim 13 wherein the cell is a mammalian cell.
Claim 17
Dependent Claim
15 words
The composition of claim 13 wherein the cell is a chinese hamster ovary cell line.
Claim 18
Dependent Claim
78 words
A process for producing the LHR which comprises transforming a host cell with a nucleic acid able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity, culturing the transformed cell and recovering said LHR from the cell culture.
Claim 19
Dependent Claim
78 words
A process for producing the LHR which comprises transforming a host cell with a nucleic acid able to hybridize under stringent conditions to the complement of a sequence encoding the carbohydrate binding domain, the epidermal growth factor domain, or a complement binding domain of the LHR having the amino acid sequence shown in FIG. 1 or FIG. 2 and encoding a polypeptide having LHR biological activity, culturing the transformed cell and recovering said LHR from the cell culture.
Claim 20
Dependent Claim
13 words
The process of claim 16 wherein the host cell is a eukaryotic cell.
Claim 21
Dependent Claim
10 words
The process of claim 16 wherein the LHR is HuLHR.
Claim 22
Dependent Claim
10 words
The process of claim 16 wherein the LHR is MLHR.
Claim 23
Dependent Claim
20 words
The process of claim 16 wherein the LHR is secreted into the culture medium and recovered from the culture medium.
Claim 24
Dependent Claim
28 words
A DNA sequence greater than about 10 bp, capable of hybridizing under stringent conditions to the complement of the DNA sequence shown in FIG. 1 or FIG. 2.
Claim 25
Dependent Claim
28 words
A DNA sequence greater than about 10 bp, capable of hybridizing under stringent conditions to the complement of the DNA sequence shown in FIG. 1 or FIG. 2.
Claim 26
Dependent Claim
50 words
The DNA sequence of claim 21, wherein the stringent conditions are overnight incubation at 42° C. in a solution comprising: 20% formamide, 5XSSC (150 mM NaCl, 15 mM trisodium citrate). 50 mM sodium phosphate (pH 7.6), 5X Denhardts solution, 10% dextran sulfate, and 20 μg/ml denatured, sheared salmon sperm DNA.
Claim 27
Dependent Claim
14 words
The DNA sequence of claim 21, wherein the fragment is a biologically active fragment
Claim 28
Dependent Claim
17 words
The DNA sequence of claim 21, wherein the fragment is from the coding region of the LHR.
Claim 29
Dependent Claim
13 words
The DNA sequence of claim 21, ligated to DNA from a non-human source.
Claim 30
Dependent Claim
29 words
The DNA sequence of claim 21, wherein the fragment comprises a fragment of the DNA sequence of FIG. 1 or FIG. 2 which is greater than about 10 bp.
Claim 31
Dependent Claim
29 words
The DNA sequence of claim 21, wherein the fragment comprises a fragment of the DNA sequence of FIG. 1 or FIG. 2 which is greater than about 20 bp.
Claim 32
Dependent Claim
29 words
The DNA sequence of claim 21, wherein the fragment comprises a fragment of the DNA sequence of FIG. 1 or FIG. 2 which is greater than about 50 bp.
Claim 33
Dependent Claim
29 words
The DNA sequence of claim 21, wherein the fragment comprises a fragment of the DNA sequence of FIG. 1 or FIG. 2 which is greater than about 100 bp.
Claim 34
Dependent Claim
15 words
The DNA sequence of claim 21, wherein the fragment comprises an LHR carbohydrate binding domain.
Claim 35
Dependent Claim
16 words
The DNA sequence of claim 21, wherein the fragment comprises an LHR epidermal growth factor domain.
Claim 36
Dependent Claim
14 words
The DNA sequence of claim 21, wherein the fragment comprises a complement binding domain.
Claim 37
Dependent Claim
27 words
A DNA sequence coding for the HuLHR having the amino acid sequence shown in FIG. 1, which DNA is substantially free of DNA encoding other human polypeptides.
Claim 38
Dependent Claim
27 words
A DNA sequence coding for the HuLHR having the amino acid sequence shown in FIG. 1, which DNA is substantially free of DNA encoding other human polypeptides.
References Cited (Backward Citations)
US5002873A
Cited
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