Patent US5096822A successfully refreshed from Google Patents.
US5096822A
Expired / Lapsed
US
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Bovine embryo medium
Assignee: PROTEIN GENETICS Inc
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Filing: 1990-07-26
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Granted: 1992-03-17
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Expires: 2010-07-26
Abstract
Abstract The present invention is directed to a bovine embryo culture medium. The medium includes a culture solution containing the nutritional substances necessary to support a bovine embryo. One of the nutritional substances is hemicalcium lactate in an amount effective to maintain the health and viability of the bovine embryo. The culture medium is a preferred nuclear transfer medium.
Inventors
- 👤 Charles F. Rosenkrans, Jr.
- 👤 Carol L. Keefer
- 👤 Michelle M. Sims
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:09
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603; C12N5/0604; C12N2500/00; C12N2500/05; C12N2500/10; C12N2500/12; C12N2500/14; C12N2500/30; C12N2500/36; C12N2500/70; C12N2500/80; C12N2500/84
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
42 words
A bovine embryo culture medium comprising a culture solution containing the nutritional substances necessary to support a bovine embryo, wherein one of said nutritional substances is hemicalcium lactate in an amount effective to maintain the health and viability of the bovine embryo.
Claim 2
Dependent Claim
42 words
A bovine embryo culture medium comprising a culture solution containing the nutritional substances necessary to support a bovine embryo, wherein one of said nutritional substances is hemicalcium lactate in an amount effective to maintain the health and viability of the bovine embryo.
Claim 3
Dependent Claim
42 words
The bovine embryo culture medium of claim 1, wherein the remaining nutritional substances are salts, sodium bicarbonate, and fatty acid free bovine serum albumin, the remaining nutritional substances being in amounts effective to maintain the health and viability of the bovine embryo.
Claim 4
Dependent Claim
17 words
The bovine embryo culture medium of claim 2, wherein the salts are sodium chloride and potassium chloride.
Claim 5
Dependent Claim
34 words
The bovine embryo culture medium of claim 3, in which the salts are present in concentrations of approximately 0 mM to 155 mM sodium chloride and approximately 0 mM to 25 mM potassium chloride.
Claim 6
Dependent Claim
24 words
The bovine embryo culture medium of claim 2, wherein the sodium bicarbonate is present in a concentration of approximately 5 mM to 40 mM.
Claim 7
Dependent Claim
25 words
The bovine embryo culture medium of claim 2, wherein the fatty-acid free BSA is present in a concentration of approximately 1 mg/ml to 6 mg/ml.
Claim 8
Dependent Claim
24 words
The bovine embryo culture medium of claim 1, wherein the hemicalcium lactate is present in a concentration of approximately 1 mM to 10 mM.
Claim 9
Dependent Claim
14 words
The bovine embryo culture medium of claim 1, which further contains a cryopreservation agent.
Claim 10
Dependent Claim
27 words
The bovine embryo culture medium of claim 2, which further contains amino acids in amounts effective to maintain the health, viability and development of the bovine embryo.
Claim 11
Dependent Claim
12 words
The bovine embryo culture medium of claim 1, which is glucose free.
Claim 12
Dependent Claim
12 words
The bovine embryo culture medium of claim 1, which is serum free.
Claim 13
Dependent Claim
23 words
The bovine embryo culture medium of claim 1, wherein the culture solution comprises antibiotically effective amounts of penicillin, streptomycin, gentamicin sulfate or fungizone.
References Cited (Backward Citations)
US4994384A
Cited
US5057420A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-03-17 | 1995-09-16 | 1996-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-03-17 | 1999-09-16 | 2000-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-03-17 | 2003-09-16 | 2004-03-17 | $7700.00 | Window Closed / Lapsed |