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Method of using an opaque plastic microscope slide for epi-fluorescent microscopy

Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1988-11-03
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Granted: 1992-03-10
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Expires: 2008-11-03
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Abstract

Abstract Disclosed is a novel microscope slide. The slide of this invention is a plastic microscope slide which is optically opaque and substantially non-fluorescent. The slide of this invention has particular utility in epi-fluorescent microscopy.

Inventors

  • 👤 Perry Strongin

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-09 18:37
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; G; G02; G02B; G02B21/00; G02B21/34; B01L2300/00; B01L2300/08; B01L2300/0809; B01L2300/0822

Claims Analysis

Total Claims: 8
Claim 1
Independent Claim 85 words
A method of conducting epi-fluorescent microscopy for testing for the presence or absence of a compound in a test sample contained on a slide by directing exciting radiation onto said sample under conditions suitable to stimulate fluorescence and monitoring the test sample for said fluorescence wherein the improvement comprises employing a plastic microscope slide comprising a body with first and second sides, said body being composed of a plastic containing a sufficient amount of carbon black to render said body optically opaque and substantially non-fluorescent.
Claim 2
Independent Claim 85 words
A method of conducting epi-fluorescent microscopy for testing for the presence or absence of a compound in a test sample contained on a slide by directing exciting radiation onto said sample under conditions suitable to stimulate fluorescence and monitoring the test sample for said fluorescence wherein the improvement comprises employing a plastic microscope slide comprising a body with first and second sides, said body being composed of a plastic containing a sufficient amount of carbon black to render said body optically opaque and substantially non-fluorescent.
Claim 3
Dependent Claim 13 words
A method according to claim 1 wherein the plastic microscope slide is solvent-resistant.
Claim 4
Dependent Claim 16 words
A method according to claim 1 wherein the plastic microscope slide contains one or more cavities.
Claim 5
Dependent Claim 26 words
A method according to claim 1 wherein the plastic is selected from the group consisting of polyolefins, polyamides, polyacrylamides, polyesters, polyacrylic esters, polycarbonates, polytetrafluoroethylene and polyvinylacetate.
Claim 6
Dependent Claim 12 words
A method according to claim 1 wherein the plasic is a polyolefin.
Claim 7
Dependent Claim 22 words
A method according to claim 5 wherein the polyolefin is derived from C 2 -C 5 olefins including copolymers and terpolymers thereof.
Claim 8
Dependent Claim 11 words
A method according to claim 6 wherein the polyolefin is polypropylene.

References Cited (Backward Citations)

US4587213A Cited
US4207554A Cited
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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1995-03-10 1995-09-09 1996-03-10 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1999-03-10 1999-09-09 2000-03-10 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 2003-03-10 2003-09-09 2004-03-10 $7700.00 Window Closed / Lapsed

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