Patent US5093240A successfully refreshed from Google Patents.
US5093240A
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Variant aequorin genes and process for producing variant aequorin proteins
Assignee: JNC Corp
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Filing: 1987-10-08
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Granted: 1992-03-03
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Expires: 2007-10-08
Abstract
Abstract Various variants of photoprotein aequorin (pAQ440), useful for elucidating the mechanism of its luminescence and thereby extending the possibility of concrete applications of aequorin protein, and a process for producing variant aequorin proteins are provided, which variants are obtained by converting base(s) in a specified order of the base arrangement of aequorin gene into other base(s), or by deleting a certain bases in specified continued orders thereof, according to site-specific mutagenesis method.
Inventors
- 👤 Satoshi Inouye
- 👤 Kouichi Kurose
- 👤 Yoshiyuki Sakaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 22:30
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/43504; C07K14/43595
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
205 words
A DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR19## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 220th nucleotide is converted from G to C; (ii) the 238th nucleotide is converted from G to A; (iii) the 307th nucleotide is converted from C to T and the 308th nucleotide is converted from A to T; (iv) the 499th nucleotide is converted from G to C; (v) the 568th nucleotide is converted from T to C; (vi) the 569th nucleotide is converted from G to C; (vii) the 590th nucleotide is converted from G to C; (viii) the 607th nucleotide is converted from G to C; (ix) the 625th nucleotide is converted from G to A; (x) the 616th nucleotide is converted from G to C and the 625th nucleotide is converted from G to A; (xi) the 674th nucleotide is converted from G to C; (xii) the 205th through the 207th nucleotides are deleted (GAT); and (xiii) the 592th through the 594th nucleotides are deleted (GAT).
Claim 2
Dependent Claim
205 words
A DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR19## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 220th nucleotide is converted from G to C; (ii) the 238th nucleotide is converted from G to A; (iii) the 307th nucleotide is converted from C to T and the 308th nucleotide is converted from A to T; (iv) the 499th nucleotide is converted from G to C; (v) the 568th nucleotide is converted from T to C; (vi) the 569th nucleotide is converted from G to C; (vii) the 590th nucleotide is converted from G to C; (viii) the 607th nucleotide is converted from G to C; (ix) the 625th nucleotide is converted from G to A; (x) the 616th nucleotide is converted from G to C and the 625th nucleotide is converted from G to A; (xi) the 674th nucleotide is converted from G to C; (xii) the 205th through the 207th nucleotides are deleted (GAT); and (xiii) the 592th through the 594th nucleotides are deleted (GAT).
Claim 3
Dependent Claim
258 words
A process for producing a variant aequorin photoprotein, said process comprising the steps of preparing a DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR20## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 220th nucleotide is converted from G to C; (ii) the 238th nucleotide is converted from G to A; (iii) the 307th nucleotide is converted from C to T and the 308th nucleotide is converted from A to T; (iv) the 499th nucleotide is converted from G to C; (v) the 568th nucleotide is converted from T to C; (vi) the 569th nucleotide is converted from G to C; (vii) the 590th nucleotide is converted from G to C; (viii) the 607th nucleotide is converted from G to C; (ix) the 625th nucleotide is converted from G to A; (x) the 616th nucleotide is converted from G to C and the 625th nucleotide is converted from G to A; (xi) the 674th nucleotide is converted from G to C; (xii) the 205the through the 207th nucleotides are deleted (GAT); and (xiii) the 592th through the 594th nucleotides are deleted (GAT), transforming a bacterial cell with said DNA molecule, wherein the bacterial cell is capable of producing the variant aequorin photoprotein from said DNA molecule, cultivating the bacterial cell, and obtaining the variant aequorin photoprotein from the bacterial cell.
Claim 4
Dependent Claim
258 words
A process for producing a variant aequorin photoprotein, said process comprising the steps of preparing a DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR20## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 220th nucleotide is converted from G to C; (ii) the 238th nucleotide is converted from G to A; (iii) the 307th nucleotide is converted from C to T and the 308th nucleotide is converted from A to T; (iv) the 499th nucleotide is converted from G to C; (v) the 568th nucleotide is converted from T to C; (vi) the 569th nucleotide is converted from G to C; (vii) the 590th nucleotide is converted from G to C; (viii) the 607th nucleotide is converted from G to C; (ix) the 625th nucleotide is converted from G to A; (x) the 616th nucleotide is converted from G to C and the 625th nucleotide is converted from G to A; (xi) the 674th nucleotide is converted from G to C; (xii) the 205the through the 207th nucleotides are deleted (GAT); and (xiii) the 592th through the 594th nucleotides are deleted (GAT), transforming a bacterial cell with said DNA molecule, wherein the bacterial cell is capable of producing the variant aequorin photoprotein from said DNA molecule, cultivating the bacterial cell, and obtaining the variant aequorin photoprotein from the bacterial cell.
Claim 5
Dependent Claim
143 words
A DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR21## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 569th nucleotide is converted from G to C and the 590th nucleotide is converted from G to C; (ii) the 590th nucleotide is converted from G to C and the 674th nucleotide is converted from G to C; (iii) the 674th nucleotide is converted from G to C and the 569th nucleotide is converted from G to C; (iv) the 569th nucleotide is converted from G to C, the 590th nucleotide is converted from G to C, and the 674th nucleotide is converted from G to C.
Claim 6
Dependent Claim
143 words
A DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR21## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 569th nucleotide is converted from G to C and the 590th nucleotide is converted from G to C; (ii) the 590th nucleotide is converted from G to C and the 674th nucleotide is converted from G to C; (iii) the 674th nucleotide is converted from G to C and the 569th nucleotide is converted from G to C; (iv) the 569th nucleotide is converted from G to C, the 590th nucleotide is converted from G to C, and the 674th nucleotide is converted from G to C.
Claim 7
Dependent Claim
196 words
A process for producing a variant aequorin photoprotein, said process comprising the steps of preparing a DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR22## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 569th nucleotide is converted from G to C and the 590th nucleotide is converted from G to C; (ii) the 590th nucleotide is converted from G to C and the 674the nucleotide is converted from G to C; (iii) the 674th nucleotide is converted from G to C and the 569th nucleotide is converted from G to C; (iv) the 569th nucleotide is converted from G to C, the 590th nucleotide is converted from G to C, and the 674th nucleotide is converted from G to C, transforming a bacterial cell with said DNA molecule, wherein the bacterial cell is capable of producing the variant aequorin photoprotein from said DNA molecule, cultivating the bacterial cell, and obtaining the variant aequorin photoprotein from the bacterial cell.
Claim 8
Dependent Claim
196 words
A process for producing a variant aequorin photoprotein, said process comprising the steps of preparing a DNA molecule encoding a variant aequorin photoprotein, said DNA molecule comprising a nucleotide sequence derived from the following nucleotide sequence of pAQ440 aequorin gene: ##STR22## wherein said derived nucleotide sequence varies from the nucleotide sequence of pAQ440 aequorin gene according to at least one member selected from the group consisting of the following: (i) the 569th nucleotide is converted from G to C and the 590th nucleotide is converted from G to C; (ii) the 590th nucleotide is converted from G to C and the 674the nucleotide is converted from G to C; (iii) the 674th nucleotide is converted from G to C and the 569th nucleotide is converted from G to C; (iv) the 569th nucleotide is converted from G to C, the 590th nucleotide is converted from G to C, and the 674th nucleotide is converted from G to C, transforming a bacterial cell with said DNA molecule, wherein the bacterial cell is capable of producing the variant aequorin photoprotein from said DNA molecule, cultivating the bacterial cell, and obtaining the variant aequorin photoprotein from the bacterial cell.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1995-03-03 | 1995-09-02 | 1996-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-03-03 | 1999-09-02 | 2000-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-03-03 | 2003-09-02 | 2004-03-03 | $7700.00 | Window Closed / Lapsed |