Patent US5091518A successfully refreshed from Google Patents.
US5091518A
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Beta retinoic acid response elements compositions and assays
Assignee: Salk Institute for Biological Studies
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Filing: 1989-11-16
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Granted: 1992-02-25
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Expires: 2009-11-16
Abstract
Abstract The present invention provide transcriptional control regions, expression vectors comprising said regions, mammalian cells transformed to transcribe and express genes from said vectors, and various methods of assaying compounds for hormone agonist or antagonist activity, all based on discovery of response elements of the β-retinoic acid receptor and of the activation of said response elements in all mammalian cells without need to transform the cells to express the receptor independently of endogenous expression thereof.
Inventors
- 👤 Henry M. Sucov
- 👤 Ronald M. Evans
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/502; G01N33/5023; C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70567; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; G01N33/74; G01N33/743; C12N2830/00; C12N2830/001; C12N2830/002; C12N2830/60; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
39 words
A substantially pure DNA having the sequence: 5'-GTTCAC-n.sub.1 n.sub.2 n.sub.3 n.sub.4 n.sub.5 -GTTCAC-3', wherein n 1 , n 2 , n 3 , n 4 , and n 5 are each independently selected from A, T, C, or G.
Claim 2
Dependent Claim
39 words
A substantially pure DNA having the sequence: 5'-GTTCAC-n.sub.1 n.sub.2 n.sub.3 n.sub.4 n.sub.5 -GTTCAC-3', wherein n 1 , n 2 , n 3 , n 4 , and n 5 are each independently selected from A, T, C, or G.
Claim 3
Dependent Claim
28 words
A DNA according to claim 1 wherein n 1 is C, n 2 is G, n 3 is A, n 4 is A, and n 5 is A.
Claim 4
Dependent Claim
10 words
A DNA according to claim 2 having the sequence: 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCAGCTT-3'.
Claim 5
Dependent Claim
10 words
A DNA according to claim 3 having the sequence: 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TC-GCAT-AGCTT-3'.
Claim 6
Dependent Claim
10 words
A DNA according to claim 4 having the sequence: 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCGCAT-ATATT-AGCTT-3'.
Claim 7
Dependent Claim
52 words
A substantially pure DNA construct comprising the DNA of claim 1 operatively linked to a promoter which is not normally subject to transcriptional activation by retinoic acid; wherein the DNA and the promoter are operatively linked so as to confer transcriptional activation activity on said promoter in the presence of retinoic acid.
Claim 8
Dependent Claim
62 words
A substantially pure DNA construct comprising a DNA segment selected from: 5'-GTTCAC-CGAAA-GTTCAC-3', 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCAGCTT-3', 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TC-GCAT-AGCTT-3', or 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCGCAT-ATATT-AGCTT-3'; wherein said DNA segment is operatively linked to a promoter which is not normally subject to transcriptional activation by retinoic acid; wherein the DNA and the promoter are operatively linked so as to confer transcriptional activation activity on said promoter in the presence of retinoic acid.
Claim 9
Dependent Claim
62 words
A substantially pure DNA construct comprising a DNA segment selected from: 5'-GTTCAC-CGAAA-GTTCAC-3', 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCAGCTT-3', 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TC-GCAT-AGCTT-3', or 5'-AAGCTTAAGG-GTTCAC-CGAAA-GTTCAC-TCGCAT-ATATT-AGCTT-3'; wherein said DNA segment is operatively linked to a promoter which is not normally subject to transcriptional activation by retinoic acid; wherein the DNA and the promoter are operatively linked so as to confer transcriptional activation activity on said promoter in the presence of retinoic acid.
Claim 10
Dependent Claim
19 words
A DNA construct according to claim 6 wherein the promoter is the delta-MTV promoter of mouse mammary tumor virus.
Claim 11
Dependent Claim
19 words
A DNA construct according to claim 7 wherein the promoter is the delta-MTV promoter of mouse mammary tumor virus.
Claim 12
Dependent Claim
19 words
A substantially pure DNA construct comprising the DNA construct of claim 9 linked operatively for transcription to a gene.
Claim 13
Dependent Claim
33 words
A vector for the expression of a protein of interest in a mammalian cell, said vector comprising the substantially pure DNA construct of claim 10, wherein said gene encodes the protein of interest.
Claim 14
Dependent Claim
33 words
A vector for the expression of a protein of interest in a mammalian cell, said vector comprising the substantially pure DNA construct of claim 10, wherein said gene encodes the protein of interest.
Claim 15
Dependent Claim
19 words
A vector according to claim 11 wherein the protein of interest is selected from luciferase, chloramphenicol acetyltransferase, and beta-galactosidase.
Claim 16
Dependent Claim
19 words
A vector according to claim 12 wherein the protein of interest is selected from luciferase, chloramphenicol acetyltransferase, and beta-galactosidase.
References Cited (Backward Citations)
No citation records indexed.
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| 3.5 Years (Year 4) | 1995-02-24 | 1995-08-26 | 1996-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-24 | 1999-08-26 | 2000-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-24 | 2003-08-26 | 2004-02-25 | $7700.00 | Window Closed / Lapsed |