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US5089415A
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Method for clotting heparized blood by the addition of protamine, thrombin and snake venom
Assignee: International Technidyne Corp
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Filing: 1990-09-17
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Granted: 1992-02-18
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Expires: 2010-09-17
Abstract
Abstract A cocktail reagent preparation for the rapid production of serum contains thrombin, snake venom, and protamine sulfate. The preparation employs very small quantities of clot promoting substances which behave in a synergistic manner such that rapid clotting of highly heparinized blood is achieved without altering the chemical analysis of the blood enzymes, proteins, sugars, or electrolytes. Thus, clinicians who rely upon the results of such tests can more closely monitor organ and tissue function and adjust patient therapies accordingly.
Inventors
- 👤 Frank M. La Duca
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 02:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N33/96; G01N2333/00; G01N2333/435; G01N2333/46; G01N2333/4609; G01N2333/4613; G01N2333/4636; G01N2333/464; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/974; G01N2400/00; G01N2400/10; G01N2400/38; G01N2400/40; G01N2496/00; G01N2496/05; G01N2496/80
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
148 words
A method for the rapid production of serum from a highly heparinized patient, said serum to be chemically tested to provide blood chemistry results, comprising: collecting a blood sample from said highly heparinized patient; mixing a sufficiently small quantity of a clot promoting cocktail with said blood sample such that said cocktail does not alter said blood chemistry results, said cocktail comprising thrombin in a concentration range of about 0.2-3.0 units per milliliter, a protamine salt in a concentration range of about 0.02-0.08 milligrams per milliliter and a snake venom in a concentration range of about 0.005-0.2 milligrams per milliliter which snake venom is capable of converting fibrinogen to fibrin and is unaffected by the presence of heparin; waiting approximately five minutes during which time said blood sample clots; centrifuging said clotted blood sample to form a serum supernatant; and isolating said serum supernatant for blood chemical testing.
Claim 2
Independent Claim
148 words
A method for the rapid production of serum from a highly heparinized patient, said serum to be chemically tested to provide blood chemistry results, comprising: collecting a blood sample from said highly heparinized patient; mixing a sufficiently small quantity of a clot promoting cocktail with said blood sample such that said cocktail does not alter said blood chemistry results, said cocktail comprising thrombin in a concentration range of about 0.2-3.0 units per milliliter, a protamine salt in a concentration range of about 0.02-0.08 milligrams per milliliter and a snake venom in a concentration range of about 0.005-0.2 milligrams per milliliter which snake venom is capable of converting fibrinogen to fibrin and is unaffected by the presence of heparin; waiting approximately five minutes during which time said blood sample clots; centrifuging said clotted blood sample to form a serum supernatant; and isolating said serum supernatant for blood chemical testing.
Claim 3
Dependent Claim
27 words
The method according to claim 1, wherein said snake venom is selected from the family of snakes known as the Genus: Agkistroden, Bothrops, Crotalus, Echis, and Trimeresurus.
Claim 4
Dependent Claim
17 words
The method according to claim 2, wherein said snake venom is obtained from the snake Bothrops atrox.
Claim 5
Dependent Claim
14 words
The method according to claim 1, wherein thrombin is obtained from a bovine source.
Claim 6
Dependent Claim
31 words
The method according to claim 1, wherein said mixture contains 0.5 units of thrombin per milliliter, 0.01 milligrams of snake venom per milliliter, and 0.05 milligrams of protamine sulfate per milliliter.
References Cited (Backward Citations)
JPS581460A
Cited
JPS6153567A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-02-17 | 1995-08-19 | 1996-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-17 | 1999-08-19 | 2000-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-17 | 2003-08-19 | 2004-02-18 | $7700.00 | Window Closed / Lapsed |