Patent US3401611A successfully refreshed from Google Patents.
Patent US5089111A successfully refreshed from Google Patents.
US5089111A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrophoretic sieving in gel-free media with dissolved polymers
Assignee: Bio Rad Laboratories Inc
•
Filing: 1990-09-27
•
Granted: 1992-02-18
•
Expires: 2010-09-27
Abstract
Abstract Separation of sample ions on the basis of molecular size is achieved by electrophoresis in an aqueous solution containing a dissolved non-crosslinked polymer. The polymer has a molecular weight range which overlaps that of the sample ions being separated. Species which vary in molecular weight but not in charge/mass ratio are separated by this method, which is of particular interest in high performance electrophoresis in capillary columns where the use of gels would be awkward and inconvenient.
Inventors
- 👤 Ming D. Zhu
- 👤 Jeng-Chyh Chen
- 👤 Stellan Hjerten
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 21:02
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
124 words
A method of separating a mixture of sample ions of varying molecular weights in a sample into components, said method comprising electrophoretically passing said sample through a separation column containing a gel-free aqueous solution of a water-soluble polymer selected from the group consisting of cellulose derivatives, saccharide-based and substituted saccharide-based polymers, polysilanes, polyvinylalcohol and polyvinylpyrrolidone, said polymer having a molecular weight of about 10,000 to about 2,000,000, said molecular weight being within a range of about 0.1 to about 200 times the average molecular weight of said sample ions in said mixture, the concentration of said polymer in said solution being sufficient to retard the flow of said species through said separation column to degrees which vary with the molecular weights of said species.
Claim 2
Independent Claim
124 words
A method of separating a mixture of sample ions of varying molecular weights in a sample into components, said method comprising electrophoretically passing said sample through a separation column containing a gel-free aqueous solution of a water-soluble polymer selected from the group consisting of cellulose derivatives, saccharide-based and substituted saccharide-based polymers, polysilanes, polyvinylalcohol and polyvinylpyrrolidone, said polymer having a molecular weight of about 10,000 to about 2,000,000, said molecular weight being within a range of about 0.1 to about 200 times the average molecular weight of said sample ions in said mixture, the concentration of said polymer in said solution being sufficient to retard the flow of said species through said separation column to degrees which vary with the molecular weights of said species.
Claim 3
Dependent Claim
37 words
A method in accordance with claim 1 in which said sample ions are macromolecular species, and said concentration of said polymer is sufficient to increase the retention time of said macromolecular species in said column by 25%.
Claim 4
Dependent Claim
40 words
A method in accordance with claim 1 in which said sample ions are macromolecular species, and said concentration of said polymer is sufficient to increase the retention time of said macromolecular species in said column by at least about 50%.
Claim 5
Dependent Claim
32 words
A method in accordance with claim 1 in which said polymer has an average molecular weight which is between the lowest and highest molecular weights of said sample ions in said mixture.
Claim 6
Dependent Claim
27 words
A method in accordance with claim 1 in which said polymer has a molecular weight range which is at least coextensive with that of said sample ions.
Claim 7
Dependent Claim
41 words
A method in accordance with claim 1 in which said polymer has an average molecular weight which is within a range of about 0.2 to about 20 times the average of the lowest and highest molecular weights of said sample ions.
Claim 8
Dependent Claim
41 words
A method in accordance with claim 1 in which said polymer has an average molecular weight which is within a range of about 0.5 to about 2 times the average of the lowest and highest molecular weights of said sample ions.
Claim 9
Dependent Claim
26 words
A method in accordance with claim 1 in which said separation column is a capillary tube with an internal diameter of less than about 200 microns.
Claim 10
Dependent Claim
29 words
A method in accordance with claim 1 in which said separation column is a capillary tube with an internal diameter of from about 25 microns to about 50 microns.
Claim 11
Dependent Claim
55 words
A method in accordance with claim 1 in which said separation column is a capillary tube with an internal diameter of less than about 100 microns, and the passing of said sample therethrough is achieved by applying a voltage of at least about 100 volts per centimeter of capillary tube length across said capillary tube.
Claim 12
Dependent Claim
55 words
A method in accordance with claim 1 in which said separation column is a capillary tube with an internal diameter of less than about 100 microns, and the passing of said sample therethrough is achieved by applying a voltage of at least about 300 volts per centimeter of capillary tube length across said capillary tube.
Claim 13
Dependent Claim
43 words
A method in accordance with claim 1 in which said polymer is a water-soluble cellulose derivative characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 15 centipoise to about 17,000 centipoise at 25° C.
Claim 14
Dependent Claim
43 words
A method in accordance with claim 1 in which said polymer is a water-soluble cellulose derivative characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 1,000 centipoise to about 10,000 centipoise at 25° C.
Claim 15
Dependent Claim
59 words
A method in accordance with claim 1 in which said polymer is a water-soluble cellulose derivative characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 1,000 centipoise to about 10,000 centipoise at 25° C., and the concentration of said polymer in said solution is at least about 0.1% by weight.
Claim 16
Dependent Claim
61 words
A method in accordance with claim 1 in which said polymer is a water-soluble cellulose derivative characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 1,000 centipoise to about 10,000 centipoise at 25° C., and the concentration of said polymer in said solution is from about 0.1% to about 10% by weight.
Claim 17
Independent Claim
111 words
A method of separating a mixture of polynucleotide chains in a sample, said polynucleotide chains each containing from about 10 to about 10,000 base pairs, said method comprising electrophoretically passing said sample through a capillary column containing a gel-free aqueous solution of a substantially linear polymer selected from the group consisting of methyl cellulose, hydroxypropyl methyl cellulose, hydroxyethyl methyl cellulose, and hydroxybutyl methyl cellulose, said polymer characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 1,000 centipoise to about 10,000 centipoise at 25° C., and the concentration of said polymer in said solution is from about 0.1% to about 0.5% by weight.
Claim 18
Independent Claim
111 words
A method of separating a mixture of polynucleotide chains in a sample, said polynucleotide chains each containing from about 10 to about 10,000 base pairs, said method comprising electrophoretically passing said sample through a capillary column containing a gel-free aqueous solution of a substantially linear polymer selected from the group consisting of methyl cellulose, hydroxypropyl methyl cellulose, hydroxyethyl methyl cellulose, and hydroxybutyl methyl cellulose, said polymer characterized in terms of the viscosity of a 2% aqueous solution thereof being within a range of about 1,000 centipoise to about 10,000 centipoise at 25° C., and the concentration of said polymer in said solution is from about 0.1% to about 0.5% by weight.
Claim 19
Dependent Claim
29 words
A method in accordance with claim 1 in which said polymer is a member selected from the group consisting of cellulose derivatives, saccharide-based polymers, substituted saccharide-based polymers, and polyvinylalcohol.
Claim 20
Dependent Claim
26 words
A method in accordance with claim 1 in which said polymer is a member selected from the group consisting of saccharide-based polymers and substituted saccharide-based polymers.
Claim 21
Dependent Claim
14 words
A method in accordance with claim 1 in which said polymer is a polyvinylalcohol.
References Cited (Backward Citations)
US4305798A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-02-17 | 1995-08-19 | 1996-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-02-17 | 1999-08-19 | 2000-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-02-17 | 2003-08-19 | 2004-02-18 | $7700.00 | Window Closed / Lapsed |