US5079352A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Purified thermostable enzyme
Assignee: Roche Molecular Systems Inc
•
Filing: 1990-05-15
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Granted: 1992-01-07
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Expires: 2010-05-15
Abstract
Abstract Recombinant DNA vectors that encode a thermostable DNA polymerase are useful in the recombinant production of thermostable DNA polymerase. The recombinant thermostable polymerase is preferred for use in the production of DNA in a polymerase chain reaction. Especially useful vectors encode the ˜94,000 dalton thermostable DNA polymerase from thermus aquaticus.
Inventors
- 👤 David H. Gelfand
- 👤 Susanne Stoffel
- 👤 Frances C. Lawyer
- 👤 Randall K. Saiki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 00:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/70; C12N9/00; C12N9/10; C12N9/12; C12N9/1241; C12N9/1252; C12N9/1276; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12Q1/68; C12Q1/6813; C12Q1/6827; C12Q1/6844; C12Q1/6858; C12Q1/686; C12Q1/6876; C12Q1/6881; C12Q1/6883
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
14 words
A recombinant DNA sequence that encodes the thermostable DNA polymerase activity of Thermus aquaticus.
Claim 2
Dependent Claim
14 words
A recombinant DNA sequence that encodes the thermostable DNA polymerase activity of Thermus aquaticus.
Claim 3
Dependent Claim
11 words
The recombinant DNA sequence of claim 1 shown in FIG. 1.
Claim 4
Dependent Claim
16 words
A recombinant DNA sequence that encodes amino acid residues 290 through 832 shown in FIG. 1.
Claim 5
Dependent Claim
16 words
A recombinant DNA sequence that encodes amino acid residues 290 through 832 shown in FIG. 1.
Claim 6
Dependent Claim
19 words
The recombinant DNA sequence of claim 3 that encodes amino acid residues 4 through 832 shown in FIG. 1.
Claim 7
Dependent Claim
36 words
A recombinant DNA vector that comprises the DNA sequence of claim 1 and can be used to drive expression of the thermostable DNA polymerase activity of Thermus aquaticus in a host cell transformed with the vector.
Claim 8
Dependent Claim
10 words
The recombinant vector of claim 5 that is plasmid pLSG1.
Claim 9
Dependent Claim
10 words
The recombinant vector of claim 5 that is plasmid pLSG2.
Claim 10
Dependent Claim
10 words
The recombinant vector of claim 5 that is plasmid pLSG5.
Claim 11
Dependent Claim
10 words
The recombinant vector of claim 5 that is plasmid pLSG6.
Claim 12
Dependent Claim
10 words
The recombinant vector of claim 5 that is plasmid pLSG10.
Claim 13
Dependent Claim
36 words
A recombinant DNA vector that comprises the DNA sequence of claim 3 and can be used to drive expression of a protein that has thermostable DNA polymerase activity in a host cell transformed with the vector.
Claim 14
Dependent Claim
10 words
The recombinant vector of claim 11 that is plasmid pLSG8.
Claim 15
Dependent Claim
10 words
The recombinant vector of claim 11 that is plasmid pFC82.
Claim 16
Dependent Claim
10 words
The recombinant vector of claim 11 that is plasmid pFC85.
Claim 17
Dependent Claim
36 words
A recombinant DNA vector that comprises the DNA sequence of claim 4 and can be used to drive expression of a protein that has thermostable DNA polymerase activity in a host cell transformed with the vector.
Claim 18
Dependent Claim
10 words
The recombinant vector of claim 15 that is plasmid pLSG7.
Claim 19
Dependent Claim
17 words
A recombinant vector selected from the group consisting of plasmid pFC83, phage CH35:Taq #4-2, and plasmid pSYC1578.
Claim 20
Dependent Claim
17 words
A recombinant vector selected from the group consisting of plasmid pFC83, phage CH35:Taq #4-2, and plasmid pSYC1578.
Claim 21
Dependent Claim
10 words
A host cell transformed with a vector of claim 5.
Claim 22
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG1.
Claim 23
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG2.
Claim 24
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG5.
Claim 25
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG6.
Claim 26
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG7.
Claim 27
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG8.
Claim 28
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pLSG10.
Claim 29
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pFC82.
Claim 30
Dependent Claim
10 words
The host cell of claim 18 that is E. coli/pFC85.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1995-01-06 | 1995-07-08 | 1996-01-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1999-01-06 | 1999-07-08 | 2000-01-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2003-01-06 | 2003-07-08 | 2004-01-07 | $7700.00 | Window Closed / Lapsed |