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US5076472A
Expired / Lapsed
US
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Cleaning cycle for flow cytometers
Assignee: Becton Dickinson and Co
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Filing: 1990-12-14
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Granted: 1991-12-31
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Expires: 2010-12-14
Abstract
Abstract A method and apparatus for cleaning cellular contamination in flow cytometer comprising the sequential use of a strong oxidizing solution, particle-free neutral pH fluid and a weak acid.
Inventors
- 👤 Hans-Joachim Gross
- 👤 Robert A. Hoffman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 09:22
CPC Classifications
A; A61; A61L; A61L2/00; A61L2/16; A61L2/18; B; B01; B01L; B01L13/00; B01L13/02; B01L2300/00; B01L2300/08; B01L2300/0861; B01L2300/0867; B01L2400/00; B01L2400/06; B01L2400/0622; B01L2400/0633; B01L3/00; B01L3/50; B01L3/502; B01L3/5027; G; G01; G01N; G01N35/00; G01N35/10; G01N35/1095; G01N35/1097; Y; Y10; Y10T; Y10T137/00; Y10T137/2931; Y10T137/3115; Y10T137/3127
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
126 words
An apparatus attached to the inlet of the fluidics system of a flow cytometer comprising: a first reservoir, comprising a first inlet for accepting air and a first outlet for discharging fluid into the inlet of the fluidics system; a second reservoir, comprising a second inlet for accepting air and a second outlet for discharging fluid into the inlet of the fluidics system; a third reservoir, comprising a third inlet for accepting air and a third outlet for discharging fluid into the inlet of the fluidics system; means for regulating air into said first, second and third reservoirs, upstream from said first, second and third inlets; and means for regulating fluid from said first, second and third reservoirs downstream from said first, second and third outlets.
Claim 2
Independent Claim
126 words
An apparatus attached to the inlet of the fluidics system of a flow cytometer comprising: a first reservoir, comprising a first inlet for accepting air and a first outlet for discharging fluid into the inlet of the fluidics system; a second reservoir, comprising a second inlet for accepting air and a second outlet for discharging fluid into the inlet of the fluidics system; a third reservoir, comprising a third inlet for accepting air and a third outlet for discharging fluid into the inlet of the fluidics system; means for regulating air into said first, second and third reservoirs, upstream from said first, second and third inlets; and means for regulating fluid from said first, second and third reservoirs downstream from said first, second and third outlets.
Claim 3
Dependent Claim
15 words
The apparatus of claim 1 wherein said means for regulating fluid is a four-way valve.
Claim 4
Dependent Claim
15 words
The apparatus of claim 1 wherein said means for regulating air is a four-way valve.
Claim 5
Dependent Claim
14 words
The apparatus of claim 1 wherein said first reservoir comprises a strong oxidizing solution.
Claim 6
Dependent Claim
16 words
The apparatus of claim 1 wherein said second reservoir comprises a particle free neutral pH fluid.
Claim 7
Dependent Claim
13 words
The apparatus of claim 1 wherein said third reservoir comprises a weak acid.
Claim 8
Dependent Claim
44 words
The apparatus of claim 1 further comprising: means for adding sheath fluid and fluid from said first, and second and third reservoirs into the inlet of the fluidics system, downstream of said means for regulating fluid, comprising a fourth inlet and a fourth outlet.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-30 | 1995-07-01 | 1995-12-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-30 | 1999-07-01 | 1999-12-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-30 | 2003-07-01 | 2003-12-31 | $7700.00 | Window Closed / Lapsed |