US5075217A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
Assignee: Marshfield Clinic Inc
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Filing: 1989-04-21
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Granted: 1991-12-24
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Expires: 2009-04-21
Abstract
Abstract The present invention is directed to a product and process for characterizing the human genome utilizing polymorphic DNA fragments containing (dC-dA)nx(dG-dT)n. The process comprises identifying polymorphic DNA fragments containing the above tandem repeat sequence followed by characterizing admixtures of genomic material by amplification of fragments containing the tandem repeat sequence using substantially unique and non-polymorphic primer sequences and appropriate separation of the so amplified fragments.
Inventors
- 👤 James L. Weber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:18
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6844; C12Q1/6858; C12Q1/6876; C12Q2600/00; C12Q2600/156
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
93 words
A method of analyzing or typing polymorphic DNA fragments which contain (dC-dA) n .(dG-dT) n repeats which are specific to particular loci in the genome comprising: amplifying at least one polymorphic DNA fragment containing at least one (dC-dA) n .(dG-dT) n segment using the polymerase chain reaction with a DNA sample which includes the said DNA fragment as a template, and with two oligonucleotide primers sufficiently complementary to non-repeated sequences at the ends of said fragment within said sample to hybridize therewith, and separating and characterizing amplified fragments on the basis of size.
Claim 2
Independent Claim
93 words
A method of analyzing or typing polymorphic DNA fragments which contain (dC-dA) n .(dG-dT) n repeats which are specific to particular loci in the genome comprising: amplifying at least one polymorphic DNA fragment containing at least one (dC-dA) n .(dG-dT) n segment using the polymerase chain reaction with a DNA sample which includes the said DNA fragment as a template, and with two oligonucleotide primers sufficiently complementary to non-repeated sequences at the ends of said fragment within said sample to hybridize therewith, and separating and characterizing amplified fragments on the basis of size.
Claim 3
Dependent Claim
14 words
The method of claim 1 further comprising labeling or marking said polymorphic DNA fragments.
Claim 4
Dependent Claim
13 words
The method of claim 1 wherein said primers are obtained by direct synthesis.
Claim 5
Dependent Claim
10 words
The method of claim 1 wherein said primers are end-labeled.
Claim 6
Dependent Claim
26 words
The method of claim 1 wherein more than one DNA fragment containing at least one segment of (dC-dA) n .(dG-dT) n is amplified and characterized simultaneously.
Claim 7
Dependent Claim
45 words
The method of claim 1 used to establish the identity, pedigree, or relationship of an individual comprising comparison of said amplified fragments with amplified fragments from the DNA of one or more control individuals wherein the control DNA samples have been similarly amplified and characterized.
Claim 8
Dependent Claim
16 words
The method of claim 1 wherein the amplified fragments are separated and characterized by gel electrophoresis.
Claim 9
Dependent Claim
12 words
The method of claim 1 wherein the DNA sample is genomic DNA.
Claim 10
Dependent Claim
12 words
The method of claim 1 wherein the DNA sample is cloned DNA.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-23 | 1995-06-24 | 1995-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-23 | 1999-06-24 | 1999-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-23 | 2003-06-24 | 2003-12-24 | $7700.00 | Window Closed / Lapsed |