US4666828A
Expired / Lapsed
US
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Test for Huntington's disease
Assignee: General Hospital Corp
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Filing: 1984-08-15
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Granted: 1987-05-19
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Expires: 2004-08-15
Abstract
Abstract A method for detecting the presence of the gene for Huntington's disease in a subject which comprises analyzing the human chromosome 4 of the subject for a DNA polymorphism, preferably a restriction fragment length polymorphism (RFLP), linked to Huntington's Disease.
Inventors
- 👤 James F. Gusella
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 00:55
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6883; C12Q1/6813; C12Q1/6827; C12Q1/683; C12Q2600/00; C12Q2600/156; C12Q2600/172; Y; Y10; Y10S; Y10S436/00; Y10S436/811
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
33 words
A method for detecting the presence in a subject of the gene for Huntington's Disease which comprises: analyzing the human chromosome 4 of said subject for a DNA polymorphism linked to Huntington's Disease.
Claim 2
Independent Claim
33 words
A method for detecting the presence in a subject of the gene for Huntington's Disease which comprises: analyzing the human chromosome 4 of said subject for a DNA polymorphism linked to Huntington's Disease.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein said polymorphism is a restriction fragment length polymorphism (RFLP).
Claim 4
Dependent Claim
86 words
The method of claim 2 wherein said step of claim 2 wherein said step of analyzing is carried out by: (a) digesting DNA from said subject with a restriction endonuclease enzyme; (b) separating fragments obtained from said digestion; (c) detecting said RFLP with a hybridization probe containing sequence information capable of hybridizing to and identifying said RFLP, thereby generating a restriction pattern; and (d) correlating the presence or absence of said RFLP in said digest with the respective presence or absence of the Huntington's Disease gene.
Claim 5
Dependent Claim
11 words
The method of claim 3 wherein said probe is detectably labelled.
Claim 6
Dependent Claim
20 words
The method of claim 3 wherein at least two different digestions with two different restriction endonuclease enzymes are carried out.
Claim 7
Dependent Claim
14 words
The method of claim 3 wherein several probes are utilized to detect several RFLP's.
Claim 8
Dependent Claim
32 words
The method of claim 3 wherein the restriction pattern for said subject is compared with the corresponding restriction pattern for family members showing segregation between the Huntington's Disease gene and said RFLP.
Claim 9
Dependent Claim
53 words
The method of claim 7 wherein the restriction pattern for said subject is compared to the corresponding restriction pattern for a parent of said subject which is unaffected by Huntington's Disease, a parent of said subject which is affected by Huntington's Disease and sibling of said subject which is affected by Huntington's Disease.
Claim 10
Dependent Claim
11 words
The method of claim 3 wherein said probe is radiolabelled G8.
Claim 11
Dependent Claim
13 words
The method of claim 3 wherein said separation is by agarose gel electrophoresis.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-05-18 | 1990-11-17 | 1991-05-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-18 | 1994-11-17 | 1995-05-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-18 | 1998-11-17 | 1999-05-19 | $7700.00 | Window Closed / Lapsed |