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US5073627A
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Fusion proteins comprising GM-CSF and IL-3
Assignee: Immunex Corp
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Filing: 1990-08-14
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Granted: 1991-12-17
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Expires: 2010-08-14
Abstract
Abstract A fusion protein is disclosed which comprises GM-CSF and IL-3. Such fusion proteins more biologically active than GM-CSF or IL-3 alone or GM-CSF and IL-3 combined.
Inventors
- 👤 Benson M. Curtis
- 👤 Linda S. Park
- 👤 David J. Cosman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:13
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/5403; C07K14/53; C07K14/535; C07K19/00; A; A61; A61K; A61K38/00; C07K2319/00; C07K2319/01; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S530/00; Y10S530/808
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
35 words
A fusion protein having a formula selected from the group consisting of R.sub.1 --R.sub.2,R.sub.2 --R.sub.1,R.sub.1 --L--R.sub.2 and R.sub.2 --L--R.sub.1 wherein R 1 is GM-CSF; R 2 is IL-3; and L is a linker peptide sequence.
Claim 2
Dependent Claim
35 words
A fusion protein having a formula selected from the group consisting of R.sub.1 --R.sub.2,R.sub.2 --R.sub.1,R.sub.1 --L--R.sub.2 and R.sub.2 --L--R.sub.1 wherein R 1 is GM-CSF; R 2 is IL-3; and L is a linker peptide sequence.
Claim 3
Dependent Claim
26 words
A fusion protein according to claim 1, wherein said linker sequence comprises amino acids selected from the group consisting of Gly, Asn, Ser, Thr and Ala.
Claim 4
Dependent Claim
20 words
A fusion protein according to claim 2, wherein the length of said linker sequence is 5 to 15 amino acids.
Claim 5
Dependent Claim
30 words
A fusion protein according to claim 3 selected from the group consisting of amino acid residues 1-271 depicted in FIG. 1 and amino acid residues 1-275 depicted in FIG. 2.
Claim 6
Dependent Claim
29 words
A fusion protein according to claim 3, wherein the fusion protein is huGM-CSF[Leu 23 Asp 27 Glu 39 ](Gly) 4 Ser(Gly) 5 Ser/huIL-3[Pro 8 Asp 15 Asp 70 ].
Claim 7
Dependent Claim
91 words
A fusion protein according to claim 1, wherein the human GM-CSF is selected from the group consisting of huGM-CSF, huGM-CSF[Leu 23 Asp 27 Glu 39 ], huGM-CSF[Leu 23 ], huGM-CSF[Leu 23 Asp 27 ], huGM-CSF[Glu 39 ], huGM-CSF[Asp 27 Glu 39 ], huGM-CSF[Leu 23 Glu 39 ] and huGM-CSF[Asp 27 ] and human IL-3 is selected from the group consisting of huIL-3, huIL-3[Pro 8 Asp 15 Asp 70 ], huIL-3[Asp 70 ], huIL-3[Asp 15 Asp 70 ], huIL-3[Pro 8 Asp 15 ], huIL-3[Pro 8 Asp 70 ], and huIL-3[Asp 15 ].
Claim 8
Dependent Claim
8 words
A fusion protein comprising GM-CSF fused to IL-3.
Claim 9
Dependent Claim
8 words
A fusion protein comprising GM-CSF fused to IL-3.
Claim 10
Dependent Claim
20 words
A composition comprising an effective amount of a fusion protein according to claim 1, and a suitable diluent or carrier.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-16 | 1995-06-17 | 1995-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-16 | 1999-06-17 | 1999-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-16 | 2003-06-17 | 2003-12-17 | $7700.00 | Window Closed / Lapsed |