US5073340A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Depositing a binder on a solid support
Assignee: Becton Dickinson and Co
•
Filing: 1990-07-06
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Granted: 1991-12-17
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Expires: 2010-07-06
Abstract
Abstract A test device for use in determining analyte wherein binder is supported on a solid support in admixture with a marker whereby the presence and location of binder on the support can be determined prior to the assay. A control for the assay may also be applied to the support in admixture with a label.
Inventors
- 👤 Gloria J. Covington
- 👤 Timothy G. Bloomster
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 17:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
133 words
A test device for use in detecting the presence of an analyte, comprising: a casing having an aperture; a solid support comprising an upper surface and contained in said casing; a test area on the upper surface of said support and positioned in registry with said aperture; a binder for said analyte attached to said test area; a detectable chromogen marker attached to said binder, related to the presence or absence of the binder which does not interfere with said assay; and a background area on the upper surface of said support without binder and detectable marker; wherein: said marker causes the binder to be distinguishable from said support so that, before the device is used in said assay the device is examined to confirm that binder is present in said test area.
Claim 2
Dependent Claim
133 words
A test device for use in detecting the presence of an analyte, comprising: a casing having an aperture; a solid support comprising an upper surface and contained in said casing; a test area on the upper surface of said support and positioned in registry with said aperture; a binder for said analyte attached to said test area; a detectable chromogen marker attached to said binder, related to the presence or absence of the binder which does not interfere with said assay; and a background area on the upper surface of said support without binder and detectable marker; wherein: said marker causes the binder to be distinguishable from said support so that, before the device is used in said assay the device is examined to confirm that binder is present in said test area.
Claim 3
Dependent Claim
204 words
A test device for use in an assay for detecting the presence of an analyte, comprising: a solid support comprising an upper surface and a lower surface; a test area on the upper surface of said support; a binder for the analyte attached to said test area; a detectable marker attached to said binder which is related to the presence or absence of the binder, and does not interfere with said assay; and a background area on the upper surface of said support without binder and detectable marker; a control area on the upper surface of said support; an assay control attached to said control area; and a detectable label attached to said assay control which is distinguishable and does not interfere with the assay or with the detectable marker; wherein: the detectable marker causes the binder to be distinguishable from said support so that, before the device is used in said assay the device is examined to confirm that binder is present in said test area; and said label causes the assay control to be distinguishable from said support so that before the device is used in said assay the device is examined to confirm that assay control is present on said support.
Claim 4
Dependent Claim
204 words
A test device for use in an assay for detecting the presence of an analyte, comprising: a solid support comprising an upper surface and a lower surface; a test area on the upper surface of said support; a binder for the analyte attached to said test area; a detectable marker attached to said binder which is related to the presence or absence of the binder, and does not interfere with said assay; and a background area on the upper surface of said support without binder and detectable marker; a control area on the upper surface of said support; an assay control attached to said control area; and a detectable label attached to said assay control which is distinguishable and does not interfere with the assay or with the detectable marker; wherein: the detectable marker causes the binder to be distinguishable from said support so that, before the device is used in said assay the device is examined to confirm that binder is present in said test area; and said label causes the assay control to be distinguishable from said support so that before the device is used in said assay the device is examined to confirm that assay control is present on said support.
Claim 5
Dependent Claim
15 words
The test device of claim 2 wherein the assay control is a positive analyte control.
Claim 6
Dependent Claim
16 words
The test device of claim 3 wherein said control area is attached to said test area.
Claim 7
Dependent Claim
12 words
The test device of claim 2 wherein the label is a chromogen.
Claim 8
Dependent Claim
12 words
The test device of claim 5 wherein the solid support is nitrocellulose.
Claim 9
Dependent Claim
88 words
A process for producing a test device suitable for detecting the presence of an analyte, comprising: providing a solid support comprising an upper surface; contacting said upper surface with a binder for said analyte; contacting said binder with a detectable marker related to the presence or absence of the binder which does not interfere with said assay; detecting the marker to confirm that binder is present in said device; and placing the support in a casing including an aperture with the test area in registry with the aperture.
Claim 10
Dependent Claim
88 words
A process for producing a test device suitable for detecting the presence of an analyte, comprising: providing a solid support comprising an upper surface; contacting said upper surface with a binder for said analyte; contacting said binder with a detectable marker related to the presence or absence of the binder which does not interfere with said assay; detecting the marker to confirm that binder is present in said device; and placing the support in a casing including an aperture with the test area in registry with the aperture.
Claim 11
Dependent Claim
54 words
The process of claim 7 further comprising: contacting said upper surface with a mixture of an assay control; and contacting said assay control with a detectable label; wherein: said control area is distinguishable from said test area and said support, and said label is related to the presence or absence of said assay control.
Claim 12
Dependent Claim
15 words
The process of claim 8 wherein said control area is attached to said test area.
Claim 13
Dependent Claim
20 words
The process of claim 8 wherein the marker is a fluorescent material and the label is a different fluorescent material.
Claim 14
Dependent Claim
11 words
The process of claim 7 wherein the marker is a chromogen.
Claim 15
Dependent Claim
12 words
The process of claim 7 wherein the marker is a fluorescent dye.
Claim 16
Dependent Claim
10 words
The process of claim 7 wherein the support is nitrocellulose.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-12-16 | 1995-06-17 | 1995-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-12-16 | 1999-06-17 | 1999-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-12-16 | 2003-06-17 | 2003-12-17 | $7700.00 | Window Closed / Lapsed |