Patent US5066580A successfully refreshed from Google Patents.
US5066580A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Xanthene dyes that emit to the red of fluorescein
Assignee: Becton Dickinson and Co
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Filing: 1990-02-16
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Granted: 1991-11-19
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Expires: 2010-02-16
Abstract
Abstract Novel xanthene dyes are disclosed which excite in the range of 450-650 nm and which will emit to the red of fluoroscein. These dyes may be coupled to tagging agents, such as monoclonal antibodies, and used to detect cells in a sample.
Inventors
- 👤 Linda Lee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 19:37
CPC Classifications
C; C09; C09B; C09B11/00; C09B11/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/582; G01N2474/00; G01N2474/20; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S436/00; Y10S436/80; Y10S436/805
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
84 words
A method for staining cells in a sample which comprises incubating said with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of ##STR3## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR4##
Claim 2
Independent Claim
84 words
A method for staining cells in a sample which comprises incubating said with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of ##STR3## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR4##
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the tapping agent is in antibody.
Claim 4
Dependent Claim
12 words
The method of claim 2 wherein the antibody is a monoclonal antibody.
Claim 5
Dependent Claim
18 words
The method of claim 1 wherein the tagging agent is coupled to the compound via a biotin-avidin complex.
Claim 6
Dependent Claim
16 words
The method of claim 1 wherein the cells are derived from body tissue or body fluids.
Claim 7
Dependent Claim
10 words
The method of claim 1 wherein the compound is ##STR5##
Claim 8
Dependent Claim
10 words
The method of claim 1 wherein the compound is ##STR6##
Claim 9
Independent Claim
107 words
A method for identifying cells in a sample which comprises: (a) incubating said cells with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of of the formula ##STR7## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR8## (b) detecting fluorescence emitted by said bound tagging agent as a measure of the presence of said cells.
Claim 10
Independent Claim
107 words
A method for identifying cells in a sample which comprises: (a) incubating said cells with a tagging agent which specifically binds to said cells under conditions sufficient to allow said cells to bind with said tagging agent, said tagging agent being coupled to a fluorescent compound selected from the group consisting of of the formula ##STR7## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR8## (b) detecting fluorescence emitted by said bound tagging agent as a measure of the presence of said cells.
Claim 11
Dependent Claim
12 words
The method of claim 8 wherein the tagging agent is an antibody.
Claim 12
Dependent Claim
12 words
The method of claim 9 wherein the antibody is a monoclonal antibody.
Claim 13
Dependent Claim
19 words
The method of claim 8 in which the tagging agent is coupled to the compound via a biotin-avidin complex.
Claim 14
Dependent Claim
16 words
The method of claim 8 wherein the cells are derived from body tissue or body fluids.
Claim 15
Dependent Claim
13 words
The method of claim 8 wherein fluorescence is detected using a flow cytometer.
Claim 16
Dependent Claim
10 words
The method of claim 8 wherein the compound is ##STR9##
Claim 17
Dependent Claim
10 words
The method of claim 8 wherein the compound is ##STR10##
Claim 18
Independent Claim
94 words
A method for discriminating between live cells and dead cells in a sample which comprises: (a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR11## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR12## under conditions sufficient to allow uptake of said compound by said live cells; and (b) detecting fluorescence emitted by said compound as a measure of the presence of said live cells.
Claim 19
Independent Claim
94 words
A method for discriminating between live cells and dead cells in a sample which comprises: (a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR11## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR12## under conditions sufficient to allow uptake of said compound by said live cells; and (b) detecting fluorescence emitted by said compound as a measure of the presence of said live cells.
Claim 20
Dependent Claim
10 words
The method of claim 16 wherein the compound is ##STR13##
Claim 21
Dependent Claim
10 words
The method of claim 16 wherein the compound is ##STR14##
Claim 22
Independent Claim
97 words
A method for detecting an esterase activity in a sample of cells comprising a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR15## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR16## under conditions sufficient to allow uptake of said compound by said cells and cleavage of said compound by said esterase activity b) detecting fluorescence emitted by said cleaved compound as a measure of said esterase activity.
Claim 23
Independent Claim
97 words
A method for detecting an esterase activity in a sample of cells comprising a) incubating said cells with a fluorescent compound selected from the group consisting of ##STR15## wherein R is CO 2 H or SO 3 H, and X is H or Br when R is CO 2 H and X is H when R is SO 3 H; ##STR16## under conditions sufficient to allow uptake of said compound by said cells and cleavage of said compound by said esterase activity b) detecting fluorescence emitted by said cleaved compound as a measure of said esterase activity.
Claim 24
Dependent Claim
10 words
The method of claim 19 wherein the compound is ##STR17##
Claim 25
Dependent Claim
10 words
The method of claim 19 wherein the compound is ##STR18##
References Cited (Backward Citations)
US4599307A
Cited
US4727020A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1994-11-18 | 1995-05-20 | 1995-11-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-11-18 | 1999-05-20 | 1999-11-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-11-18 | 2003-05-20 | 2003-11-19 | $7700.00 | Window Closed / Lapsed |