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US5061635A
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Protein or peptide sequencing method
Assignee: City of Hope
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Filing: 1988-03-29
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Granted: 1991-10-29
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Expires: 2008-03-29
Abstract
Abstract A method and apparatus for sequencing polypeptides, including a continuous flow reactor which may include a sample bearing membrane strip.
Inventors
- 👤 John E. Shively
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 01:28
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01J; B01J19/00; B01J19/0046; B01J2219/00; B01J2219/00274; B01J2219/00277; B01J2219/00279; B01J2219/00281; B01J2219/00286; B01J2219/00351; B01J2219/00423; B01J2219/00497; B01J2219/005; B01J2219/00583; B01J2219/0059; B01J2219/00596; B01J2219/00718; B01J2219/0072; B01J2219/00725; C; C40; C40B; C40B40/00; C40B40/04; C40B40/10; C40B60/00; C40B60/14; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
114 words
The method which comprises: (1) resolving a mixture of proteins or peptides by gel electrophoresis; (2) transferring a sample peptide or protein isolated on said gel to a hydrophobic synthetic resin membrane of open porous structure upon which said sample is adsorbed; (3) staining said sample adsorbed on said membrane; (4) dividing the membrane into strips bearing portions of the stained sample; (5) placing at least one of said strips into a cylindrical sequenator reaction chamber the longitudinal axis of at least one of said strips being substantially parallel to the longitudinal axis of said cylindrical reaction chamber; (6) placing the reaction chamber in a sequenator; and (7) subjecting the sample to sequence analysis.
Claim 2
Independent Claim
114 words
The method which comprises: (1) resolving a mixture of proteins or peptides by gel electrophoresis; (2) transferring a sample peptide or protein isolated on said gel to a hydrophobic synthetic resin membrane of open porous structure upon which said sample is adsorbed; (3) staining said sample adsorbed on said membrane; (4) dividing the membrane into strips bearing portions of the stained sample; (5) placing at least one of said strips into a cylindrical sequenator reaction chamber the longitudinal axis of at least one of said strips being substantially parallel to the longitudinal axis of said cylindrical reaction chamber; (6) placing the reaction chamber in a sequenator; and (7) subjecting the sample to sequence analysis.
Claim 3
Dependent Claim
32 words
The method of claim 1 in which the gel is a sodium dodecyl sulfate gel, the synthetic resin membrane is a polyvinyl difluoride membrane and the sample is stained with Coomassie Blue.
Claim 4
Dependent Claim
23 words
The method of claim 2 in which the membrane is formed from a combination of polyvinyl difluoride and a polymeric quaternary ammonium salt.
References Cited (Backward Citations)
US4603114A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-28 | 1995-04-29 | 1995-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-28 | 1999-04-29 | 1999-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-28 | 2003-04-29 | 2003-10-29 | $7700.00 | Window Closed / Lapsed |