US5059542A
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US
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Artificial carrier particles and method for preparation thereof
Assignee: JURIDICAL FOUNDATION CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE A CORP OF JAPAN
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Filing: 1989-10-12
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Granted: 1991-10-22
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Expires: 2009-10-12
Abstract
Abstract An artificial carrier particle comprising an anionic polymer and a synthetic polyamino acid having at least one carboxylic group and at least one amino group in its side chain, the complex being insolubilized by an aldehyde crosslinking agent. The artificial carrier particle is useful in immunoassay, in particular, particle immunoassay. The artificial carrier particles are obtained by preparing an aqueous solution containing an anionic polymer and a synthetic polyamino acid comprising at least one free carboxyl group and at least one free amino group in its side chain, adjusting the pH of the solution to 3.5 to 9.5 at room temperature or at progressively increasing temperature under stirring to form solution particles of a desired particle size, and insolubilizing the particles by an aldehyde crosslinking agent.
Inventors
- 👤 Takenori Hirai
- 👤 Hirotaka Ihara
- 👤 Chuichi Hirayama
- 👤 Haruo Fuzita
- 👤 Munehiro Saisho
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:22
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/14; A61K9/16; A61K9/1605; A61K9/1629; A61K9/1641; A61K9/1652; C; C08; C08G; C08G69/00; C08G69/02; C08G69/36; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
96 words
An artificial carrier particle, comprising; a complex of (A) a synthetic polyamino acid having at least one carboxylic group and at least one amino group in its side chain, in which a molar ratio of the carboxyl group to the amino group is about 10:90 to about 90:10 and a polymerization degree of 50 to 1500; and (B) an anionic polymer selected from the group consisting of water soluble polysaccharides, polyglutamate and polyacrylate, the complex being insolubilized by an aldehyde crosslinking agent selected from the group consisting of glutaraldehyde, formaldehyde, glyoxal, crotonyl aldehyde, acrolein and acetoaldehyde.
Claim 2
Dependent Claim
96 words
An artificial carrier particle, comprising; a complex of (A) a synthetic polyamino acid having at least one carboxylic group and at least one amino group in its side chain, in which a molar ratio of the carboxyl group to the amino group is about 10:90 to about 90:10 and a polymerization degree of 50 to 1500; and (B) an anionic polymer selected from the group consisting of water soluble polysaccharides, polyglutamate and polyacrylate, the complex being insolubilized by an aldehyde crosslinking agent selected from the group consisting of glutaraldehyde, formaldehyde, glyoxal, crotonyl aldehyde, acrolein and acetoaldehyde.
Claim 3
Dependent Claim
40 words
The artificial carrier particle of claim 1, wherein the synthetic polyamino acid is an acidic polyamino acid and the amino group is introduced in the acidic polyamino acid via a part of a free carboxyl group of the polyamino acid.
Claim 4
Dependent Claim
40 words
The artificial carrier particle of claim 1, wherein the synthetic polyamino acid is a basic polyamino acid and the carboxyl group is introduced in the basic polyamino acid via a part of a free amino group of the polyamino acid.
Claim 5
Dependent Claim
25 words
The artificial carrier particle of claim 1, wherein the synthetic polyamino acid is a copolymer of an acidic polyamino acid and a basic polyamino acid.
Claim 6
Dependent Claim
26 words
The artificial carrier particle of claim 1, wherein a weight ratio of the synthetic polyamino acid to the anionic polymer is about 5:1 to about 1:5.
Claim 7
Dependent Claim
26 words
The artificial carrier particle of claim 5, wherein a weight ratio of the synthetic polyamino acid to the anionic polymer is about 2:1 to about 1:2.
Claim 8
Dependent Claim
31 words
The artificial carrier particle of claim 1, wherein the molar ratio of the carboxyl group to the amino group of the synthetic polyamino acid (A) is about 20:80 to about 60:40.
Claim 9
Dependent Claim
52 words
The artificial carrier particle of claim 1, wherein the water soluble polysaccharides are selected from the group consisting of gum arabic, carboxymethyl cellulose, sodium alginate, agar and carrageenan, wherein further the polyglutamate is sodium polyglutamate, and wherein further the polyacrylate is selected from the group of partially hydrolyzed polyacrylate and polyacryl amide.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1994-10-21 | 1995-04-22 | 1995-10-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-21 | 1999-04-22 | 1999-10-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-21 | 2003-04-22 | 2003-10-22 | $7700.00 | Window Closed / Lapsed |