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US5055562A
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Fluorocarbon chain-containing antigenic conjugates
Assignee: Seagen Inc
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Filing: 1988-02-01
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Granted: 1991-10-08
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Expires: 2008-02-01
Abstract
Abstract Fluorocarbon chain-containing linking arms can be used to conjugate haptens to protein carriers such as BSA, HSA, or antibodies without recourse to harsh chemistry. In addition, 19 F atoms serve as markers for quantitative estimation of bound haptens.
Inventors
- 👤 R. Rao Koganty
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 11:07
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/385; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6889; A61K2039/60; A61K2039/6031; A61K2039/6081; A61K2039/62; A61K2039/627
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
33 words
A method of conjugating a hapten with an immunogenic carrier which comprises forming a conjugate of the hapten and the carrier covalently linked together by a linker comprising at least one fluorocarbon chain.
Claim 2
Independent Claim
33 words
A method of conjugating a hapten with an immunogenic carrier which comprises forming a conjugate of the hapten and the carrier covalently linked together by a linker comprising at least one fluorocarbon chain.
Claim 3
Dependent Claim
12 words
The method of claim 1, wherein the carrier bears an amino function.
Claim 4
Dependent Claim
11 words
The method of claim 1, wherein the carrier is a protein.
Claim 5
Dependent Claim
18 words
The method of claim 3 in which the linker is attached to the hapten by an amide linkage.
Claim 6
Dependent Claim
48 words
The method of claim 3 in which the linker is H--Ch--(CH.sub.2).sub.m --(CF.sub.2).sub.n --R' where CH stands for chalcogen and is oxygen or sulfur, m is 2-20, n is 2-20, and R' is H or a reactive group that participates in the coupling of the linker to a protein.
Claim 7
Dependent Claim
35 words
The method of claim 3 where R' is selected from the group consisting of --CON 3 --N 3 , --CONHNH 2 ,--NHNH 2 , --NH 2 , --OH, --OMe, --OEt, --COO--Benzyl and --CF 3 .
Claim 8
Dependent Claim
48 words
The method of claim 3 in which the linker is H--CH--(CH.sub.2).sub.m --NHCO--(CF.sub.2).sub.n --R' where Ch stands for chalcogen and is oxygen or sulfur, m is 2-20, n is 2-20, and R' is H or a reactive group that participates in the coupling of the linker to a protein.
Claim 9
Dependent Claim
37 words
The method of claim 5 where R' is selected from the group consisting of --CON 3 , --N 3 , --CONHNH 2 , --NHNH 2 , --NH 2 , --OH, --OMe, --OEt, --COO--Benzyl and --CF 3 .
Claim 10
Dependent Claim
18 words
The method of claim 3 in which the linker is attached to the hapten by an ether linkage.
Claim 11
Dependent Claim
23 words
The method of claim 3 in which the linker comprises at least two aliphatic carbon chains, at least one of which is fluorinated.
Claim 12
Dependent Claim
23 words
The method of claim 8 in which at least one pair of chains is coupled together by an ether, together, or amide linkage.
Claim 13
Dependent Claim
18 words
The method of claim 3 in which the linker portion of the resulting conjugate is essentially immunologically inert.
Claim 14
Dependent Claim
19 words
An antigenic conjugate comprises a hapten, an immunogenic carrier, and a covalent linker comprising at least one fluorocarbon chain.
Claim 15
Dependent Claim
19 words
An antigenic conjugate comprises a hapten, an immunogenic carrier, and a covalent linker comprising at least one fluorocarbon chain.
Claim 16
Dependent Claim
12 words
The conjugate of claim 13, wherein the carrier bears an amino function.
Claim 17
Dependent Claim
11 words
The conjugate of claim 13, wherein the carrier is a protein.
Claim 18
Dependent Claim
48 words
The antigenic conjugate of claim 15, wherein the linker is H--Ch--(CH.sub.2).sub.m --(CF.sub.2).sub.n --R' where Ch stands for chalcogen and is oxygen or sulfur, m is 2-20, n is 2-20, and R' is H or a reactive group that participates in the coupling of the linker to a protein.
Claim 19
Dependent Claim
38 words
The antigenic conjugate of claim 16, wherein R' is selected from the group consisting of --CON 3 , --N 3 , --CONHNH 2 , --NHNH 2 , --NH 2 , --OH, --OMe, --OEt, --COO--Benzyl and --CF 3 .
Claim 20
Dependent Claim
48 words
The antigenic conjugate of claim 15, wherein the linker is H--Ch--(CH.sub.2).sub.m --NHCO--(CF.sub.2).sub.n --R' where Ch stands for chalcogen and is oxygen or sulfur, m is 2-20, n is 2-20, and R' is H or a reactive group that participates in the coupling of the linker to a protein.
Claim 21
Dependent Claim
38 words
The antigenic conjugate of claim 18 wherein R is selected from the group consisting of --CON 3 , --N 3 , --CONHNH 2 , --NHNH 2 , --NH 2 , --OH, --OMe, --OEt, --COO--Benzyl and --CF 3 .
Claim 22
Dependent Claim
21 words
The antigenic conjugate of claim 18 wherein at least one fluorine atom in said fluorocarbon chain is an F 19 .
Claim 23
Dependent Claim
15 words
The antigenic conjugate of claim 13 in which the hapten is a T antigenic determinant.
Claim 24
Dependent Claim
12 words
The method of claim 1 in which the hapten is a T-hapten.
Claim 25
Dependent Claim
13 words
The method of claim 22 in which the carrier is keyhole limpet hemocyanin.
Claim 26
Dependent Claim
13 words
The conjugate of claim 21 in which the carrier is keyhole limpet hemocyanin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-10-07 | 1995-04-08 | 1995-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-10-07 | 1999-04-08 | 1999-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-10-07 | 2003-04-08 | 2003-10-08 | $7700.00 | Window Closed / Lapsed |