Patent US5050609A successfully refreshed from Google Patents.
US5050609A
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Magnetization transfer contrast and proton relaxation and use thereof in magnetic resonance imaging
Assignee: United States Department of Health and Human Services
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Filing: 1989-04-14
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Granted: 1991-09-24
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Expires: 2009-04-14
Abstract
Abstract A nuclear magnetic resonance method is provided for monitoring and imaging the exchange of magnetization between protons in free water and protons in a relatively immobilized pool of protons in a sample. The method provides a new form of contrast for nuclear magnetic resonance imaging of samples such as biological tissues, polymers, and geological samples.
Inventors
- 👤 Robert S. Balaban
- 👤 Steven D. Wolff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 12:32
CPC Classifications
G; G01; G01R; G01R33/00; G01R33/20; G01R33/44; G01R33/48; G01R33/54; G01R33/56; G01R33/5605; G01R33/483; G01R33/4833
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
97 words
A method for obtaining spatial information about the rate of exchange of a reaction using the technique of saturation transfer by collecting NMR data from a sample in which the reaction occurs comprising: irradiating a first pool of nuclei with sufficient electromagnetic radiation to magnetically label said nuclei; electromagnetically exciting a second pool of nuclei that is in exchange with said first pool; applying magnetic field gradients to spatially resolve the NMR signal intensity of said second pool of nuclei; collecting and reading NMR data obtained from said signal; and determining said spatial information about said reaction.
Claim 2
Independent Claim
97 words
A method for obtaining spatial information about the rate of exchange of a reaction using the technique of saturation transfer by collecting NMR data from a sample in which the reaction occurs comprising: irradiating a first pool of nuclei with sufficient electromagnetic radiation to magnetically label said nuclei; electromagnetically exciting a second pool of nuclei that is in exchange with said first pool; applying magnetic field gradients to spatially resolve the NMR signal intensity of said second pool of nuclei; collecting and reading NMR data obtained from said signal; and determining said spatial information about said reaction.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein said first pool of nuclei is in smaller concentration than said second pool of nuclei.
Claim 4
Dependent Claim
24 words
The method of claim 1 wherein the data obtained from said method is ratioed with a conventional NMR image to obtain a ratio image.
Claim 5
Dependent Claim
24 words
The method of claim 3 wherein said ratio image is combined with spin-lattice relaxation data to obtain a rate constant image for the reaction.
Claim 6
Dependent Claim
46 words
The method of claim 1 wherein NMR data is collected from a nucleus selected from the group consisting of 1 H, 31 P, 13 C, 23 Na, 19 F, 2 H, 17 O, 15 O, 7 Li, 14 N, 15 N, 39 K, and 35 Cl.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the exchange is enzymatically catalyzed.
Claim 8
Dependent Claim
11 words
The method of claim 6 wherein the enzyme is creatine kinase.
Claim 9
Dependent Claim
27 words
The method of claim 1 wherein the first pool of nuclei is the gamma-phosphorus of ATP and the second pool of nuclei is the phosphorous in phosphocreatine.
Claim 10
Dependent Claim
15 words
The method of claim 1 Wherein the second pool of nuclei comprises protons in water.
Claim 11
Dependent Claim
28 words
The method of claim 1 wherein the exchange reaction involves labile protons of a compound in exchange with protons of the solution in which the compound is dissolved.
Claim 12
Dependent Claim
10 words
The method of claim 10 wherein the solvent is water.
Claim 13
Dependent Claim
28 words
The method of claim 1 wherein the first pool of nuclei is selected from the group consisting of protons of urea, protons of creatine, and protons of ammonia.
Claim 14
Dependent Claim
12 words
The method of claim 1 wherein the sample is of biological origin.
Claim 15
Dependent Claim
12 words
The method of claim 1 wherein the sample is of geologic origin.
Claim 16
Dependent Claim
11 words
The method of claim 1 wherein the sample is a polymer.
Claim 17
Dependent Claim
12 words
The method of claim 1 wherein the NMR data is spatially resolved.
Claim 18
Dependent Claim
12 words
The method of claim 1 wherein the rate of exchange is measured.
Claim 19
Independent Claim
74 words
A method for determining the rate of exchange of a proton magnetization between a macromolecule and a solvent by collecting NMR data from a sample in which said exchange occurs comprising: irradiating a first a macromolecule with sufficient electromagnetic radiation to magnetically label said nuclei; and electromagnetically excite the nuclei that is exchanging proton magnetization with said macromolecule; collecting and reading NMR data obtained from said signal; and determining the presence of said exchange.
Claim 20
Independent Claim
74 words
A method for determining the rate of exchange of a proton magnetization between a macromolecule and a solvent by collecting NMR data from a sample in which said exchange occurs comprising: irradiating a first a macromolecule with sufficient electromagnetic radiation to magnetically label said nuclei; and electromagnetically excite the nuclei that is exchanging proton magnetization with said macromolecule; collecting and reading NMR data obtained from said signal; and determining the presence of said exchange.
Claim 21
Dependent Claim
18 words
The method of claim 18 wherein said macromolecule of nuclei is in smaller concentration than said solvent nuclei.
Claim 22
Dependent Claim
10 words
The method of claim 18 wherein the solvent is wtaer.
Claim 23
Dependent Claim
29 words
The method of claim 18 wherein magnetic field gradients are applied to spatially resolve the NMR signal intensity of the solvent to reveal the spatial distribution of said exchange.
Claim 24
Dependent Claim
11 words
The method of claim 18 wherein the exchange rate is determined.
Claim 25
Dependent Claim
12 words
The method of claim 18 wherein the macromolecule is of biological origin.
Claim 26
Dependent Claim
12 words
The method of claim 18 wherein the macromolecule is of geologic origin.
Claim 27
Dependent Claim
11 words
The method of claim 18 wherein the macromolecule is a polymer.
Claim 28
Dependent Claim
16 words
The method of claim 18 wherein the macromolecule is a protein, lipid, saccharide or combined molecule.
Claim 29
Dependent Claim
17 words
The method of claim 26 wherein the second pool of nuclei comprises nuclei contained in a solvent.
Claim 30
Dependent Claim
24 words
The method of claim 18 wherein the data obtained from said method is ratioed with a conventional NMR image to obtain a ratio image.
Claim 31
Dependent Claim
27 words
The method of claim 18 wherein said ratio image is combined with spin-lattice relaxation data to obtain a rate constant image for the exchange of proton magnetization.
Claim 32
Dependent Claim
16 words
the method of claim 18 wherein said saturation transfer occurs via chemical exchange or dipolar interaction.
References Cited (Backward Citations)
No citation records indexed.
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| 3.5 Years (Year 4) | 1994-09-23 | 1995-03-25 | 1995-09-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-23 | 1999-03-25 | 1999-09-24 | $3760.00 | Window Closed / Lapsed |
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