Patent US5045531A successfully refreshed from Google Patents.
US5045531A
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Wound treatment employing biologically active ion channel forming peptides and proteins
Assignee: Magainin Sciences Inc
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Filing: 1989-12-18
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Granted: 1991-09-03
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Expires: 2009-12-18
Abstract
Abstract A process for treating a wound in a host which comprises administering to the host having a wound at least one biologically active amphiphilic peptide. The peptide is an ion channel-forming peptide and is administered in an amount effective for treating a wound in a host.
Inventors
- 👤 Barry Berkowitz
- 👤 Leonard S. Jacob
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:46
CPC Classifications
C; C07; C07K; C07K7/00; C07K7/04; C07K7/08; A; A61; A61K; A61K38/00; A61K38/04; A61K38/16; A61K38/17; A61K38/1703; A61K38/1709; A61K38/1751; A61P; A61P43/00; C07K14/00; C07K14/001; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4742
Claims Analysis
Total Claims: 35
Claim 1
Independent Claim
62 words
A process for treating a wound in a host, comprising administering to a host having a wound at least one biologically active amphiphilic peptide and/or biologically active protein, said peptide or protein being an ion channel-forming peptide or protein, said at least one biologically active amphiphilic peptide or protein being administered in an amount effective for treating a wound in the host.
Claim 2
Dependent Claim
62 words
A process for treating a wound in a host, comprising administering to a host having a wound at least one biologically active amphiphilic peptide and/or biologically active protein, said peptide or protein being an ion channel-forming peptide or protein, said at least one biologically active amphiphilic peptide or protein being administered in an amount effective for treating a wound in the host.
Claim 3
Dependent Claim
36 words
The process of claim 1 wherein the peptide is a basic polypeptide having at least sixteen amino acids, wherein said basic polypeptide includes at least eight hydrophobic amino acids and at least eight hydrophilic amino acids.
Claim 4
Dependent Claim
91 words
The process of claim 2 wherein said polypeptide comprises a chain of at least four groups of amino acids, each of said at least four groups consisting of four amino acids, wherein two of the four amino acids in each group are hydrophobic amino acids, and two of the four amino acids in each group are hydrophilic amino acids, with at least one of the hydrophilic amino acids in each group being a basic hydrophilic amino acid and the other hydrophilic amino acid being a basic or neutral hydrophilic amino acid.
Claim 5
Dependent Claim
72 words
The process of claim 3 wherein each of said groups of four amino acids is of the sequence ABCD, BCDA, CDAB, or DABC, wherein A and B are each hydrophobic amino acids and may be the same or different, one of C or D is a basic hydrophilic amino acid, and the other of C or D is a basic or neutral hydrophilic amino acid and may be the same or different.
Claim 6
Dependent Claim
12 words
The process of claim 1 wherein the peptide is a magainin peptide.
Claim 7
Dependent Claim
97 words
The process of claim 5 wherein said magainin peptide includes the following basic peptide structure: --R.sub.11 -R.sub.11 -R.sub.12 -R.sub.13 -R.sub.11 -R.sub.14 -R.sub.12 -R.sub.11 -R.sub.14 - -R.sub.12 -R.sub.11 -R.sub.11 -R.sub.11 -R.sub.14a -(R.sub.15)n-R.sub.14a -R.sub.14 -- wherein R 11 is a hydrophobic amino acid, R 12 is a basic hydrophilic amino acid, R 13 is a hydrophobic, neutral hydrophilic, or basic hydrophilic amino acid, R 14 and R 14 are hydrophobic or basic hydrophilic amino acids, R 15 is glutamic acid or aspartic acid, a hydrophobic amino acid, or a basic hydrophilic amino acid, and n is 0 or 1.
Claim 8
Dependent Claim
16 words
The process of claim 6 wherein R 13 is a hydrophobic or neutral hydrophilic amino acid.
Claim 9
Dependent Claim
13 words
The process of claim 6 wherein R 14a is a hydrophobic amino acid.
Claim 10
Dependent Claim
14 words
The process of claim 6 wherein R 15 is glutamic acid or aspartic acid.
Claim 11
Dependent Claim
73 words
The process of claim 5 wherein said magainin peptide is of the following basic peptide structure: --R.sub.14 -R.sub.11 -R.sub.14a -R.sub.12 -R.sub.11 -R.sub.11 -R.sub.12 -R.sub.13 -R.sub.11 - R.sub.14 -R.sub.12 -R.sub.11 -R.sub.11 -R.sub.12 --, wherein R 11 is a hydrophobic amino acid, R 12 is a basic hydrophilic amino acid, R 13 is a hydrophobic, neutral hydrophilic, or basic hydrophilic amino acid, and R 14 and R 14a are hydrophobic or basic hydrophilic amino acids.
Claim 12
Dependent Claim
16 words
The process of claim 10 wherein R 13 is a hydrophobic or neutral hydrophilic amino acid.
Claim 13
Dependent Claim
13 words
The process of claim 10 wherein R 14a is a hydrophobic amino acid.
Claim 14
Dependent Claim
11 words
The process of claim 1 wherein the peptide is a cecropin.
Claim 15
Dependent Claim
11 words
The process of claim 1 wherein the peptide is a sarcotoxin.
Claim 16
Dependent Claim
12 words
The process of claim 1 wherein the peptide is a XPF peptide.
Claim 17
Dependent Claim
12 words
The process of claim 1 wherein the peptide is a PGLa peptide.
Claim 18
Dependent Claim
12 words
The process of claim 1 wherein the peptide is a CPF peptide.
Claim 19
Dependent Claim
11 words
The process of claim 1 wherein the peptide is administered systemically.
Claim 20
Dependent Claim
62 words
A process for treating a wound in a host, comprising adminstering to a host having a wound at least one biologically active amphiphilic peptide selected from the class consisting of magainin peptides, cecropins, sarcotoxins, PGLa peptides, CPF peptides, and XPF peptides, said at least one biologically active amphiphilic peptide being administered in an amount effective for treating a wound in the host.
Claim 21
Dependent Claim
62 words
A process for treating a wound in a host, comprising adminstering to a host having a wound at least one biologically active amphiphilic peptide selected from the class consisting of magainin peptides, cecropins, sarcotoxins, PGLa peptides, CPF peptides, and XPF peptides, said at least one biologically active amphiphilic peptide being administered in an amount effective for treating a wound in the host.
Claim 22
Dependent Claim
11 words
The process of claim 19 wherein the peptide is magainin peptide.
Claim 23
Dependent Claim
11 words
The process of claim 19 wherein the peptide is a cecropin.
Claim 24
Dependent Claim
11 words
The process of claim 19 wherein the peptide is a sarcotoxin.
Claim 25
Dependent Claim
12 words
The process of claim 19 wherein the peptide is a CPF peptide.
Claim 26
Dependent Claim
13 words
The process of claim 19 wherein the peptide is is an XPF peptide.
Claim 27
Dependent Claim
11 words
The process of claim 5 wherein said magainin peptide is: (NH.sub.2)GIGKFLHSAKKFGKAFVGEIMNS(NH.sub.2).
Claim 28
Dependent Claim
99 words
The process of claim 20 wherein said magainin peptide includes the following basic peptide structure: --R.sub.11 -R.sub.11 -R.sub.12 -R.sub.13 -R.sub.11 -R.sub.14 -R.sub.12 -R.sub.11 -R.sub.14 - R.sub.12 -R.sub.11 -R.sub.11 -R.sub.11 -R.sub.14a -(R.sub.15).sub.n -R.sub.14a -R.sub.14 --, wherein R 11 is a hydrophobic amino acid, R 12 is a basic hydrophilic amino acid, R 13 is a hydrophobic, neutral hydrophilic, or basic hydrophilic amino acids, R 14 and R 14a are hydrophobic or basic hydrophilic amino acids, R 15 is a glutamic acid or aspartic acid, a hydrophobic amino acid, or a basic hydrophilic amino acid, and n is 0 or 1.
Claim 29
Dependent Claim
16 words
The process of claim 26, wherein R 13 is a hydrophobic or neutral hydrophilic amino acid.
Claim 30
Dependent Claim
13 words
The process of claim 26 wherein R 14a is a hydrophobic amino acid.
Claim 31
Dependent Claim
15 words
The process of claim 26 wherein R 15 is a glutamic acid or aspartic acid.
Claim 32
Dependent Claim
73 words
The process of claim 20 wherein said magainin peptide is of the following basic peptide structure. --R.sub.14 -R.sub.11 -R.sub.14a -R.sub.12 -R.sub.11 -R.sub.11 -R.sub.12 -R.sub.13 -R.sub.11 - R.sub.14 -R.sub.12 -R.sub.11 -R.sub.11 -R.sub.12, wherein R 11 is a hydrophobic amino acid, R 12 is a basic hydrophilic amino acid, R 13 is a hydrophobic, neutral hydrophilic, or basic hydrophilic amino acid, and R 14 and R 14a , are hydrophobic or basic hydrophilic amino acids.
Claim 33
Dependent Claim
16 words
The process of claim 30 wherein R 13 is a hydrophobic or neutral hydrophilic amino acid.
Claim 34
Dependent Claim
13 words
The process of claim 30 wherein R 14a is a hydrophobic amino acid.
Claim 35
Dependent Claim
11 words
The process of claim 20 wherein said magainin peptide is: (NH.sub.2)GIGKFLHSAKKFGKAFVGEIMNS(NH.sub.2).
References Cited (Backward Citations)
US4507230A
Cited
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