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US5037746A
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RNA ribozyme polymerases, and methods
Assignee: University Patents Inc
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Filing: 1989-03-16
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Granted: 1991-08-06
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Expires: 2009-03-16
Abstract
Abstract A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C 5 ) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C 5 to generate chain lengths of 10 to 11 nucleotides; longer products are also generated but at reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C 5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template.
Inventors
- 👤 Thomas R. Cech
- 👤 Arthur J. Zaug
- 👤 Michael D. Been
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 22:30
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/113; C12N15/10; C12N2310/00; C12N2310/10; C12N2310/11; C12N2310/111; C12N2310/12; C12N2310/124; C12N2310/1241
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
60 words
A method for synthesizing an RNA molecule, comprising the steps of: providing an enzymatic RNA molecule having RNA polymerase activity independent of any protein, and contacting said enzymatic RNA molecule with a separate primer RNA and a separate substrate RNA to allow said RNA polymerase activity to cause said primer RNA and said substrate RNA to form said RNA molecule.
Claim 2
Independent Claim
60 words
A method for synthesizing an RNA molecule, comprising the steps of: providing an enzymatic RNA molecule having RNA polymerase activity independent of any protein, and contacting said enzymatic RNA molecule with a separate primer RNA and a separate substrate RNA to allow said RNA polymerase activity to cause said primer RNA and said substrate RNA to form said RNA molecule.
Claim 3
Dependent Claim
23 words
The method of claim 1, wherein said enzymatic RNA molecule has the nucleotide sequence of an intervening sequence molecule formed of nucleic acid.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein said intervening sequence molecule is present in Tetrahymena.
Claim 5
Dependent Claim
13 words
The method of claim 3, wherein said Tetrahymena is of the species thermophilla.
Claim 6
Dependent Claim
22 words
The method of claim 4, wherein said enzymatic RNA molecule has an RNA sequence which is substantially identical to L-21 or L-19.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein said primer comprises at least four nucleotides.
Claim 8
Dependent Claim
13 words
The method of claim 6, wherein said primer comprises at least five nucleotides.
Claim 9
Dependent Claim
11 words
The method of claim 7, wherein said primer is a pentaribonucleotide.
Claim 10
Dependent Claim
12 words
The method of claim 6, wherein said pentaribonucleotide is pC 5 .
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-05 | 1995-02-04 | 1995-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-05 | 1999-02-04 | 1999-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-05 | 2003-02-04 | 2003-08-06 | $7700.00 | Window Closed / Lapsed |