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US5037742A
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Pseudorabies virus recombinants and their use in the production of proteins
Assignee: Bristol Myers Squibb Pharma Co
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Filing: 1986-07-18
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Granted: 1991-08-06
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Expires: 2006-07-18
Abstract
Abstract Recombinant pseudoviruses and their use in the production of proteins having medical, agricultural and industrial utilities are discussed.
Inventors
- 👤 Lynn W. Enquist
- 👤 Alan K. Robbins
- 👤 Mary E. Whealy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 13:55
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C07K2319/00; C07K2319/40; C07K2319/70; C07K2319/735; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16311; C12N2710/16322; C12N2710/16341; C12N2710/16343; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16211; C12N2740/16222
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
39 words
A recombinant pseudorabies virus comprising a foreign DNA fragment substituted for part of or the whole of a XhoI/gIII region of the viral genome wherein the foreign DNA fragment is capable of expression and codes for a desired protein.
Claim 2
Dependent Claim
39 words
A recombinant pseudorabies virus comprising a foreign DNA fragment substituted for part of or the whole of a XhoI/gIII region of the viral genome wherein the foreign DNA fragment is capable of expression and codes for a desired protein.
Claim 3
Dependent Claim
20 words
A recombinant pseudorabies virus according to claim 1 wherein the foreign DNA fragment is a Bg1II 1.43 kb HTLVIII fragment.
Claim 4
Dependent Claim
22 words
A recombinant pseudorabies virus according to claim 2 wherein the virus is PRV-24 having a biological deposit accession number ATCC VR 2145.
Claim 5
Dependent Claim
18 words
A recombinant pseudorabies virus comprising deleting in whole or in part the XhoI/gIII region of the viral genome.
Claim 6
Dependent Claim
18 words
A recombinant pseudorabies virus comprising deleting in whole or in part the XhoI/gIII region of the viral genome.
Claim 7
Dependent Claim
50 words
A recombinant pseudorabies virus according to claim 4 wherein the deletion in part begins at a first SacI restriction enzyme cleavage site within the gIII coding sequence of the viral genome and ends at a second SacI restriction enzyme cleavage site within the gIII coding sequence of the viral genome.
Claim 8
Dependent Claim
22 words
A recombinant pseudorabies virus according to claim 5 wherein the virus is PRV-2 having a biological deposit accession number ATCC VR 2143.
Claim 9
Dependent Claim
34 words
A recombinant pseudorabies virus according to claim 5 wherein an EcoRI restriction enzyme cleavage site is located at the regenerated SacI restriction enzyme cleavage site within the gIII coding sequence of the viral genome.
Claim 10
Dependent Claim
22 words
A recombinant pseudorabies virus according to claim 7 wherein the virus is PRV-1 having a biological deposit accession number ATCC VR 2142.
Claim 11
Dependent Claim
41 words
A recombinant pseudorabies virus according to claim 4 wherein the deletion in whole begins at a first XhoI restriction enzyme cleavage site and ends at a second XhoI restriction enzyme cleavage site within the gIII coding sequence of the viral genome.
Claim 12
Dependent Claim
22 words
A recombinant pseudorabies virus according to claim 9 wherein the virus is PRV-10 having a biological deposit accession number ATCC VR 2144.
Claim 13
Dependent Claim
36 words
A process for producing a desired protein comprising: (1) infecting a cell with a recombinant pseudorabies virus of claim 1; (2) culturing the infected cell until a desired protein is produced; and (3) recovering the protein.
Claim 14
Dependent Claim
18 words
A process according to claim 11 wherein the recombinant pseudorabies virus comprises a Bg1II 1.43 Kb HTLVIII fragment.
Claim 15
Dependent Claim
21 words
A process according to claim 11 wherein the recombinant pseudorabies virus is PRV-24 having a biological accession number ATCC VR 2145.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-05 | 1995-02-04 | 1995-08-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-05 | 1999-02-04 | 1999-08-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-05 | 2003-02-04 | 2003-08-06 | $7700.00 | Window Closed / Lapsed |