US5032288A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Blood collection method
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1990-05-16
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Granted: 1991-07-16
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Expires: 2010-05-16
Abstract
Abstract A method of use is disclosed of a device that causes phase separation of whole blood, using much lower centrifugal forces. As a result, leukocytes are separated from blood cells having specific gravities of 1.09 g/ml or higher. The method preferably uses a separation chamber arranged so that its long dimension or axis is parallel, not perpendicular, to the spin axis, and a valve that allows automatic removal of the ligher phase(s). In one aspect, the method separates a lighter phase from a heavier phase, for example, lymphocytes from whole blood, using a centrifugal force of reduced amount. In another aspect, DNA is extracted readily from the separated lighter phase since it is less likely that the lighter phase will be contaminated by erythrocytes as occurs in conventional separation techniques.
Inventors
- 👤 Richard L. Columbus
- 👤 Susan M. Atwood
- 👤 Deborah P. Freyler
- 👤 Harvey J. Palmer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:53
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; A; A61; A61B; A61B5/00; A61B5/15; A61B5/150007; A61B5/150015; A61B5/150022; A61B5/150206; A61B5/150221; A61B5/150251; A61B5/150343; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; A61M; A61M5/00; A61M5/178; A61M5/31; A61M5/32; A61M5/3205; A61M5/321; A61M5/322; A61M5/3221; A61M2005/3228; A61M5/50; A61M5/5086; B01L2300/00; B01L2300/08; B01L2300/0848; B01L2300/0858; B01L2400/00; B01L2400/06; B01L2400/0605; B01L2400/0616; B01L2400/08; B01L2400/084; B01L2400/086; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
98 words
A method of separating a lighter phase from a heavier phase in a two-phase liquid, comprising a) placing the two-phase liquid of a predetermined volume into a first chamber having one void dimension longer than the other orthogonal void dimensions, b) spinning said chamber about an axis that is generally parallel to said one dimension and offset from said chamber, at a rate of no greater than 400 g's, and c) while still spinning said chamber, drawing off the separated lighter phase into a second chamber adjacent to said first chamber with a valve interposed between said chambers.
Claim 2
Independent Claim
98 words
A method of separating a lighter phase from a heavier phase in a two-phase liquid, comprising a) placing the two-phase liquid of a predetermined volume into a first chamber having one void dimension longer than the other orthogonal void dimensions, b) spinning said chamber about an axis that is generally parallel to said one dimension and offset from said chamber, at a rate of no greater than 400 g's, and c) while still spinning said chamber, drawing off the separated lighter phase into a second chamber adjacent to said first chamber with a valve interposed between said chambers.
Claim 3
Dependent Claim
40 words
A method as defined in claim 1, wherein said step c) comprises the step of opening said valve solely in response to a predetermined head of liquid pressure generated on said valve by the lighter phase during said spinning step.
Claim 4
Dependent Claim
30 words
A method as defined in claim 2, wherein said head of pressure is created by increasing the centrifugal force within said chamber above the force used to achieve phase separation.
Claim 5
Dependent Claim
21 words
A method as defined in claim 1, wherein said liquid is whole blood and wherein said separated lighter phase includes lymphocytes.
References Cited (Backward Citations)
US3849072A
Cited
US3935113A
Cited
US3941699A
Cited
US3945928A
Cited
US4015775A
Cited
US4202769A
Cited
US4487700A
Cited
US4640785A
Cited
US4788154A
Cited
US4708710A
Cited
US4828716A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-07-15 | 1995-01-14 | 1995-07-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-07-15 | 1999-01-14 | 1999-07-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-07-15 | 2003-01-14 | 2003-07-16 | $7700.00 | Window Closed / Lapsed |