US4708710A
Expired / Lapsed
US
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Particle separation process
Assignee: Terumo Corp
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Filing: 1986-03-27
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Granted: 1987-11-24
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Expires: 2006-03-27
Abstract
Abstract A process for separating particles from mixtures containing the particles is disclosed. An adoptive transfer therapy process wherein mononuclear cells are separated from a mixture of blood cells by the process is also disclosed. The process comprises supporting the mixture in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture. The process is further characterized in that the displacing fluid has a density greater than or equal to the density of the preselected particles.
Inventors
- 👤 George F. Dunn, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:49
CPC Classifications
A; A61; A61M; A61M1/00; A61M1/36; A61M1/3693; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/15; A61K35/17; A61M1/3696; B; B04; B04B; B04B5/00; B04B5/04; B04B5/0442; B04B5/06; B04B2005/0471; Y; Y10; Y10T; Y10T436/00; Y10T436/11; Y10T436/111666
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
65 words
A process for separating preselected particles from a mixture containing the particles, comprising: supporting the mixture in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture; wherein the displacing fluid has a density greater than or equal to the density of the preselected particles.
Claim 2
Dependent Claim
65 words
A process for separating preselected particles from a mixture containing the particles, comprising: supporting the mixture in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating the preselected particles from the mixture; wherein the displacing fluid has a density greater than or equal to the density of the preselected particles.
Claim 3
Dependent Claim
33 words
A process as defined in claim 1, wherein the mixture is subjected to a centrifugal force to stratify the mixture before the displacing fluid is forced through the chamber against the centrifugal force.
Claim 4
Dependent Claim
23 words
A process as defined in claim 1, wherein the preselected particles have a diameter of from about 1 μm to about 0.25 cm.
Claim 5
Dependent Claim
23 words
A process as defined in claim 3, wherein the preselected particles have a diameter of from about 2 μm to about 20 μm.
Claim 6
Dependent Claim
14 words
A process as defined in claim 1, wherein the preselected particles are biological cells.
Claim 7
Dependent Claim
42 words
A process as defined in claim 5, wherein the displacing fluid is selected from the group consisting of colloidal suspensions, high molecular weight salt solutions, mixtures of polymers with high molecular weight salts, and mixtures of polysaccarides with high molecular weight salts.
Claim 8
Dependent Claim
38 words
A process as defined in claim 6, wherein biological cells having a density less than or equal to the displacing fluid are collected as said biological cells pass out of the inner centrifugal end of the centrifuge chamber.
Claim 9
Dependent Claim
26 words
A process as defined in claim 5, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 10
Dependent Claim
14 words
A process as defined in claim 1, wherein the preselected particles are blood cells.
Claim 11
Dependent Claim
14 words
A process as defined in claim 9, wherein the blood cells are mononuclear cells.
Claim 12
Dependent Claim
23 words
A process as defined in claim 10, wherein the displacing fluid is a mixture of a polysaccaride with a high molecular weight salt.
Claim 13
Dependent Claim
23 words
A process as defined in claim 10, wherein the displacing fluid has a density of from about 1.06 g/mL to about 1.08 g/mL.
Claim 14
Dependent Claim
23 words
A process as defined in claim 12, wherein the displacing fluid has a density of from about 1.07 g/mL to about 1.08 g/mL.
Claim 15
Dependent Claim
26 words
A process as defined in claim 13, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 16
Dependent Claim
38 words
A process as defined in claim 13, wherein mononuclear cells having a density less than or equal to the displacing fluid are collected as said mononuclear cells pass out of the inner centrifugal end of the centrifuge chamber.
Claim 17
Dependent Claim
18 words
A process as defined in claim 12, wherein the centrifugal force is from about 100×g to about 15,000×g.
Claim 18
Dependent Claim
18 words
A process as defined in claim 16, wherein the centrifugal force is from about 400×g to about 2,000×g.
Claim 19
Dependent Claim
26 words
A process as defined in claim 9, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 20
Dependent Claim
26 words
A process as defined in claim 10, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 21
Dependent Claim
94 words
In an adoptive transfer therapy process wherein mononuclear cells are contacted with a lymphokine to activate lymphokine-activated killer cells in the mononuclear cells, the improvement comprising separating said mononuclear cells from a mixture of blood cells by the process comprising: supporting the mixture of blood cells in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating mononuclear cells from the mixture; wherein the displacing fluid has a density greater than or equal to the density of the mononuclear cells.
Claim 22
Dependent Claim
94 words
In an adoptive transfer therapy process wherein mononuclear cells are contacted with a lymphokine to activate lymphokine-activated killer cells in the mononuclear cells, the improvement comprising separating said mononuclear cells from a mixture of blood cells by the process comprising: supporting the mixture of blood cells in a centrifuge chamber; subjecting the chamber to a centrifugal force; and forcing a displacing fluid through the chamber, against the centrifugal force, thereby separating mononuclear cells from the mixture; wherein the displacing fluid has a density greater than or equal to the density of the mononuclear cells.
Claim 23
Dependent Claim
36 words
A process as defined in claim 20, wherein the mixture of blood cells is subjected to a centrifugal force to stratify the mixture before the displacing fluid is forced through the chamber against the centrifugal force.
Claim 24
Dependent Claim
23 words
A process as defined in claim 20, wherein the displacing fluid is a mixture of a polysaccaride with a high molecular weight salt.
Claim 25
Dependent Claim
23 words
A process as defined in claim 22, wherein the displacing fluid has a density of from about 1.06 g/mL to about 1.08 g/mL.
Claim 26
Dependent Claim
23 words
A process as defined in claim 23, wherein the displacing fluid has a density of from about 1.07 g/mL to about 1.08 g/mL.
Claim 27
Dependent Claim
18 words
A process as defined in claim 23, wherein the centrifugal force is from about 100×g to about 15,000×g.
Claim 28
Dependent Claim
18 words
A process as defined in claim 25, wherein the centrifugal force is from about 400×g to about 2,000×g.
Claim 29
Dependent Claim
26 words
A process as defined in claim 26, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 30
Dependent Claim
26 words
A process as defined in claim 25, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 31
Dependent Claim
38 words
A process as defined in claim 23, wherein mononuclear cells having a density less than or equal to the displacing fluid are collected as said mononuclear cells pass out of the inner centrifugal end of the centrifuge chamber.
Claim 32
Dependent Claim
26 words
A process as defined in claim 29, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 33
Dependent Claim
26 words
A process as defined in claim 23, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
Claim 34
Dependent Claim
26 words
A process as defined in claim 20, wherein the process is conducted in a closed apparatus comprising a centrifuge chamber and storage bags connected by tubing.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-23 | 1991-05-25 | 1991-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-23 | 1995-05-25 | 1995-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-23 | 1999-05-25 | 1999-11-24 | $7700.00 | Window Closed / Lapsed |