Patent US5024949A successfully refreshed from Google Patents.
US5024949A
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Aerobic bacterial remediation of aliphatic chlorinated hydrocarbon contamination
Assignee: BioTrol Inc
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Filing: 1989-02-17
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Granted: 1991-06-18
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Expires: 2009-02-17
Abstract
Abstract A bacterium of the genus Pseudomonas which utilizes a branched chain alkyl-substituted aromatic hydrocarbon as its sole carbon and energy source and which is capable of substantially complete degradation of trichlorethylene (TCE) at a rate of up to about 32 nmol hr-1 mg cells-1 based upon the dry weight of the cells, and methods utilizing the bacterium for the detoxification of TCE-contaminated material.
Inventors
- 👤 George D. Hegeman
- 👤 David G. Nickens
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:26
CPC Classifications
C; C02; C02F; C02F3/00; C02F3/02; C02F3/12; C02F3/1205; C02F3/1231; C02F3/34; Y; Y02; Y02W; Y02W10/00; Y02W10/10; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
100 words
A method of detoxifying a liquid contaminated with trichlorethylene (TCE), said method comprising the step of introducing a bacterium of the genus Pseudomonas into a contaminated medium comprising: a) an amount of a branched chain alkyl-substituted aromatic hydrocarbon effective to sustain growth of the bacterium, and b) TCE, wherein the bacterium is capable of substantially complete degradation of the TCE at a rate of up to about 32 nmol hr -1 mg cells -1 based upon the dry weight of the cells, and further wherein the bacterium is capable of sustained growth solely on the branched chain alkyl-substituted aromatic hydrocarbon.
Claim 2
Independent Claim
100 words
A method of detoxifying a liquid contaminated with trichlorethylene (TCE), said method comprising the step of introducing a bacterium of the genus Pseudomonas into a contaminated medium comprising: a) an amount of a branched chain alkyl-substituted aromatic hydrocarbon effective to sustain growth of the bacterium, and b) TCE, wherein the bacterium is capable of substantially complete degradation of the TCE at a rate of up to about 32 nmol hr -1 mg cells -1 based upon the dry weight of the cells, and further wherein the bacterium is capable of sustained growth solely on the branched chain alkyl-substituted aromatic hydrocarbon.
Claim 3
Dependent Claim
14 words
The method of claim 1 wherein the branched chain alkyl-substituted aromatic hydrocarbon is isobutylbenzene.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the TCE is present in the contaminated medium at an initial concentration of up to at least about 1.11 mM.
Claim 5
Dependent Claim
32 words
The method of claim 1 wherein the amount of the branched chain alkyl-substituted aromatic hydrocarbon effective to sustain growth of the bacterium is about 0.07-0.2 percent by volume of the contaminated medium.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein the substantially complete degradation of the TCE occurs concomitantly with the growth of the bacterium.
Claim 7
Dependent Claim
20 words
The method of claim 1 wherein the bacterium has the characteristics of ATCC deposit No. 53817 and the mutations thereof.
Claim 8
Dependent Claim
82 words
A biologically pure strain of the genus Pseudomonas comprising a bacterium cultured in a medium comprising a branched chain alkyl-substituted aromatic hydrocarbon as a carbon and energy source of the bacterium, wherein the cultured bacterium is capable of substantially complete gradation of TCE at a rate of up to about 32 nmol hr -1 mg cells -1 based upon the dry weight of the cells, and wherein the bacterium is capable of sustained growth solely on the branched chain alkyl-substituted aromatic hydrocarbon.
Claim 9
Dependent Claim
82 words
A biologically pure strain of the genus Pseudomonas comprising a bacterium cultured in a medium comprising a branched chain alkyl-substituted aromatic hydrocarbon as a carbon and energy source of the bacterium, wherein the cultured bacterium is capable of substantially complete gradation of TCE at a rate of up to about 32 nmol hr -1 mg cells -1 based upon the dry weight of the cells, and wherein the bacterium is capable of sustained growth solely on the branched chain alkyl-substituted aromatic hydrocarbon.
Claim 10
Dependent Claim
16 words
The biologically pure strain of claim 7 wherein the branched chain alkyl-substituted aromatic hydrocarbon is isobutylbenzene.
Claim 11
Dependent Claim
32 words
The biologically pure strain of claim 7 wherein the cultured bacterium is capable of substantially complete degradation of TCE present at an initial concentration of up to at least about 1.11 mM.
Claim 12
Dependent Claim
22 words
The biologically pure strain of claim 7 wherein the bacterium has the characteristics of ATCC deposit No. 53817 and the mutations thereof.
Claim 13
Independent Claim
81 words
A method of detoxifying a liquid contaminated with trichlorethylene (TCE), said method comprising the step of introducing a bacterium of the genus Pseudomonas into a contaminated medium comprising: a) an amount of a hydrocarbon mixture effective to sustain growth of the bacterium, wherein the hydrocarbon mixture comprises a branched chain alkyl-substituted aromatic hydrocarbon; and b) TCE at an initial concentration of up to at least about 1.11 mM, wherein the bacterium is capable of sustained growth solely on the hydrocarbon mixture.
Claim 14
Independent Claim
81 words
A method of detoxifying a liquid contaminated with trichlorethylene (TCE), said method comprising the step of introducing a bacterium of the genus Pseudomonas into a contaminated medium comprising: a) an amount of a hydrocarbon mixture effective to sustain growth of the bacterium, wherein the hydrocarbon mixture comprises a branched chain alkyl-substituted aromatic hydrocarbon; and b) TCE at an initial concentration of up to at least about 1.11 mM, wherein the bacterium is capable of sustained growth solely on the hydrocarbon mixture.
Claim 15
Dependent Claim
19 words
The method of claim 11 wherein the bacterium has the characteristics of ATCC No. 53817 and the mutations thereof.
Claim 16
Dependent Claim
19 words
The method of claim 11 wherein the bacterium has the characteristics of ATCC No. 53819 and the mutations thereof.
Claim 17
Dependent Claim
14 words
The method of claim 11 wherein the bacterium is Pseudomonas sp. ALS 3 .
Claim 18
Dependent Claim
24 words
The method of claim 11 wherein the bacterium is capable of providing a substantially linear kinetic course of substantially complete removal of the TCE.
Claim 19
Dependent Claim
19 words
The method of claim 15 wherein the bacterium has the characteristics of ATCC No. 53817 and the mutations thereof.
Claim 20
Dependent Claim
19 words
The method of claim 15 wherein the bacterium has the characteristics of ATCC No. 53819 and the mutations thereof.
Claim 21
Dependent Claim
14 words
The method of claim 15 wherein the bacterium is Pseudomonas sp. ALS 3 .
Claim 22
Dependent Claim
20 words
The method of claim 11 wherein the initial concentration of TCE is from an undetectable level to about 2.79 mM.
Claim 23
Dependent Claim
19 words
The method of claim 19 wherein the bacterium has the characteristics of ATCC No. 53817 and the mutations thereof.
Claim 24
Dependent Claim
19 words
The method of claim 19 wherein the bacterium has the characteristics of ATCC No. 53819 and the mutations thereof.
Claim 25
Dependent Claim
14 words
The method of claim 19 wherein the bacterium is Pseudomonas sp. ALS 3 .
References Cited (Backward Citations)
No citation records indexed.
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| 3.5 Years (Year 4) | 1994-06-17 | 1994-12-17 | 1995-06-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-06-17 | 1998-12-17 | 1999-06-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-06-17 | 2002-12-17 | 2003-06-18 | $7700.00 | Window Closed / Lapsed |