Patent US5015476A successfully refreshed from Google Patents.
US5015476A
Expired / Lapsed
US
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Immunization implant and method
Assignee: Heska Corp
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Filing: 1989-08-11
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Granted: 1991-05-14
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Expires: 2009-08-11
Abstract
Abstract Antigenic parasites are encapsulated in semipermeable polymeric envelope membranes to prepare implant capsules for immunizing an animal against parasitic infection. An animal may be immunized against parasitic infection by implanting into the animal the implant capsule at a location in the animal sufficient to stimulate a protective immune response in the animal to the parasite antigen.
Inventors
- 👤 Kent C. Cochrum
- 👤 Lynnor B. Stevenson
- 👤 Scott R. King
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 23:07
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0024; A61K9/48; A61K9/50; A61K9/5073; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 41
Claim 1
Independent Claim
48 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is a mammalian parasite within the Phyla Protozoa, Platyhelminthes or Nemathelminthes.
Claim 2
Dependent Claim
48 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is a mammalian parasite within the Phyla Protozoa, Platyhelminthes or Nemathelminthes.
Claim 3
Dependent Claim
69 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is an organism selected from the group consisting of parasites from the groups Toxocara, Giardia, Leishmania, Trypanosoma, Entamoeba, Toxoplasma, Plasmodium, Schistosoma, Taenia, Dipylidium, Hymenolepis, Echinococcus, Ascaris, Ancyclostomatidae, Strongylida, Trichuris, Filaroidea and Onchocerca.
Claim 4
Dependent Claim
69 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is an organism selected from the group consisting of parasites from the groups Toxocara, Giardia, Leishmania, Trypanosoma, Entamoeba, Toxoplasma, Plasmodium, Schistosoma, Taenia, Dipylidium, Hymenolepis, Echinococcus, Ascaris, Ancyclostomatidae, Strongylida, Trichuris, Filaroidea and Onchocerca.
Claim 5
Dependent Claim
63 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is selected from the group consisting of Toxocara canis, Strongylus vulgaris, Dirofilaria immitis, Fasciola hepatica, Leishmania braziliensis, Leishmania donovani, Leishmania mexicana, Trypanosoma cruzi and Toxoplasma gondii.
Claim 6
Dependent Claim
63 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said parasite is selected from the group consisting of Toxocara canis, Strongylus vulgaris, Dirofilaria immitis, Fasciola hepatica, Leishmania braziliensis, Leishmania donovani, Leishmania mexicana, Trypanosoma cruzi and Toxoplasma gondii.
Claim 7
Dependent Claim
26 words
The implant capsule of claim 1, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof.
Claim 8
Dependent Claim
73 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof and said polycationic polyamino acid is polyarginine, polylysine, or polyornithine.
Claim 9
Dependent Claim
73 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof and said polycationic polyamino acid is polyarginine, polylysine, or polyornithine.
Claim 10
Dependent Claim
17 words
The implant capsule of claim 4, wherein said polyanionic polyamino acid is polyaspartic acid, or polyglutamic acid.
Claim 11
Dependent Claim
69 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof and said intermediate bonding material comprises a polysaccharide-containing material and a biocompatible gellable metal hydroxide.
Claim 12
Dependent Claim
69 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof and said intermediate bonding material comprises a polysaccharide-containing material and a biocompatible gellable metal hydroxide.
Claim 13
Dependent Claim
26 words
The implant capsule of claim 7, wherein said intermediate bonding layer comprises a mixture of aluminum hydroxide with agar, agarose, alginate, or chitosan or mixtures thereof.
Claim 14
Dependent Claim
16 words
The implant capsule of claim 1, wherein said intermediate bonding layer is a polycationic polyamino acid
Claim 15
Dependent Claim
13 words
The implant capsule of claim 9, wherein said polycationic polyamino acid is polylysine.
Claim 16
Dependent Claim
43 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said intermediate bonding material contains an immunologic adjuvant.
Claim 17
Dependent Claim
43 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said intermediate bonding material contains an immunologic adjuvant.
Claim 18
Dependent Claim
18 words
The implant capsule of claim 11, wherein said adjuvant is an antigenic polymer, a lymphokine or a cytokine.
Claim 19
Dependent Claim
64 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a biocompatible biodegradable polymer selected from the group consisting of poly(lactide-co-glycolide) polymers, poly(epsiloncaprolactone) polymers and copolymers thereof, poly(hydroxybutyric acid)-poly(hydroxyvalerate) copolymers and poly(Cbz-tyrosine-tyrosinehexyl ester) iminocarbonate.
Claim 20
Dependent Claim
64 words
An implant capsule for immunizing an animal against parasite infection, comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes, wherein said polymeric envelope membrane is a biocompatible biodegradable polymer selected from the group consisting of poly(lactide-co-glycolide) polymers, poly(epsiloncaprolactone) polymers and copolymers thereof, poly(hydroxybutyric acid)-poly(hydroxyvalerate) copolymers and poly(Cbz-tyrosine-tyrosinehexyl ester) iminocarbonate.
Claim 21
Dependent Claim
58 words
An implant capsule for immunizing an animal against parasitic infection, wherein said implant capsule is prepared by the steps of: (a) suspending a parasite capable of producing antigens in an aqueous growth medium; (b) coating said parasite with a gellable positively charged intermediate bonding material; and (c) encapsulating said coated parasite with a polyanionic semipermeable polymeric envelope, membrane.
Claim 22
Dependent Claim
58 words
An implant capsule for immunizing an animal against parasitic infection, wherein said implant capsule is prepared by the steps of: (a) suspending a parasite capable of producing antigens in an aqueous growth medium; (b) coating said parasite with a gellable positively charged intermediate bonding material; and (c) encapsulating said coated parasite with a polyanionic semipermeable polymeric envelope, membrane.
Claim 23
Dependent Claim
40 words
The implant capsule of claim 14, wherein said coating step is conducted by contacting said suspended parasite with a solution of aluminum hydroxide, a polycationic polyamino acid, agar, agarose, alginate, chitosan or mixtures thereof to form a coated parasite droplet.
Claim 24
Dependent Claim
34 words
The implant capsule of claim 15, wherein said coated parasite droplet is further contacted with a solution of a polyanionic polyamino acid polymer and then with a solution of a polycationic polyamino acid polymer.
Claim 25
Dependent Claim
27 words
The implant capsule of claim 16, wherein said polyanionic polyamino acid is polyaspartic acid, or polyglutamic acid and said polycationic polyamino acid is polylysine, polyarginine or polyornithine.
Claim 26
Dependent Claim
54 words
The implant capsule of claim 15, wherein said parasite is suspended in a mixture of aluminum hydroxide with at least one of agar, agarose, alginate or chitosan, and said droplets are contacted with a solution of polylysine and calcium ions, whereby said alginate is gelled and said polylysine forms said semipermeable polymeric envelope membrane.
Claim 27
Dependent Claim
45 words
The implant capsule of claim 15, wherein said parasite is suspended in the mixture of aluminum hydroxide with at least one of agar, agarose, alginate or chitosan, and said droplets are contacted with a solution of a polyanionic amino acid to encapsulate said coated parasite.
Claim 28
Independent Claim
64 words
A method of immunizing an animal against parasitic infection, comprising the step of implanting into an animal, an implant capsule comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes; at a location in said animal sufficient to stimulate a protective immune response in said animal to said parasite antigen.
Claim 29
Independent Claim
64 words
A method of immunizing an animal against parasitic infection, comprising the step of implanting into an animal, an implant capsule comprising at least one antigen-producing parasite coated with a positively charged intermediate bonding material and encapsulated within one or more semipermeable polymeric envelope membranes; at a location in said animal sufficient to stimulate a protective immune response in said animal to said parasite antigen.
Claim 30
Dependent Claim
19 words
The method of claim 20, wherein said parasite is a mammalian parasite within the Phyla Protozoa, Platyhelminthes or Nemathelminthes.
Claim 31
Dependent Claim
39 words
The method of claim 21, wherein said parasite is an organism selected from the group consisting of parasites from the groups Toxocara, Giardia, Leishmania, Trypanosoma, Entamoeba, Plasmodium, Schistosoma, Taenia, Dipylidium, Hymenolepis, Echinococcus, Ascaris, Ancyclostomatidae, Strongylida, Trichuris, Filaroidea and Onchocerca.
Claim 32
Dependent Claim
25 words
The method of claim 20, wherein said polymeric envelope membrane is a polycationic polyamino acid polymer, a polyanionic polyamino acid polymer, or a combination thereof.
Claim 33
Dependent Claim
12 words
The method of claim 23, wherein said polycationic polyamino acid is polylysine.
Claim 34
Dependent Claim
12 words
The method of claim 23, said polyanionic polyamino acid is polyaspartic acid.
Claim 35
Dependent Claim
24 words
The method of claim 20, wherein said intermediate bonding material comprises a mixture of aluminum hydroxide with agar, agarose, alginate, chitosan or mixtures thereof.
Claim 36
Dependent Claim
13 words
The method of claim 20, wherein said intermediate bonding layer is aluminum hydroxide.
Claim 37
Dependent Claim
15 words
The method of claim 20, wherein said intermediate bonding layer is a polycationic polyamino acid.
Claim 38
Dependent Claim
12 words
The method of claim 28, wherein said polycationic polyamino acid is polylysine.
Claim 39
Dependent Claim
35 words
The method of claim 19, wherein said polymeric envelope membrane is a biocompatible biodegradable polymer selected from the group consisting of poly(lactide-co-glycolide) polymers, poly(epsilon-caprolactone) polymers and copolymers thereof, poly(hydroxybutyric acid-)poly(hydroxyvalerate) copolymers and poly(Cbz-tyrosine-tyrosine-hexyl ester) iminocarbonate.
Claim 40
Dependent Claim
14 words
The method of claim 20, wherein said intermediate bonding material contains an immunologic adjuvant.
Claim 41
Dependent Claim
18 words
The method of claim 31, wherein said immunologic adjuvant is an antigenic polymer, a lymphokine or a cytokine.
References Cited (Backward Citations)
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