Patent US2863721A successfully refreshed from Google Patents.
Patent US5011780A successfully refreshed from Google Patents.
US5011780A
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US
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In vitro embryo culture technique
Assignee: Biotechnology and Biological Sciences Research Council
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Filing: 1988-06-17
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Granted: 1991-04-30
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Expires: 2008-06-17
Abstract
Abstract Avian embryos may be cultured in vitro up to blastoderm formation, during embryonic morphogenesis and/or during embryonic growth to hatch. The invention may have applications not only in the genetic engineering of poultry, but also in the investigation of fundamental mechanisms of avian development and in the study of deleterious traits. Moreover, it may afford a desirable alternative to surgical intervention in the laying hen.
Inventors
- 👤 Margaret M. Perry
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 23:33
CPC Classifications
C; C12; C12M; C12M3/00; C12M3/10; A; A01; A01K; A01K67/00; A01K67/02; A01K45/00; C12M21/00; C12M21/10; C12M23/00; C12M23/24; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0603
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
167 words
A process for the in vitro culture of an avian embryo up to blastoderm formation, during embryonic morphogenesis and during embryonic growth to hatch, the process comprising culturing an avian embryo by a process comprising culturing a fertilized avian ovum, having a surrounding capsule of dense albumen, partially subemreged in culture medium in a first container sealed with a film having low gas permeability and not naturally associated with an resultant avian egg, up to blastoderm formation, subsequently culturing the avian embryo by a process comprising incubating said avian embryo during embryonic mrophogenesis in a culture medium in a liquid-filled liquid-impermeable second container sealed with a partially gas permeable film, and subsequently culturing the resultant avian embryo by a process comprising incubating said avian embryo during the embryonic growth phase in a closed second or third container, there being an air space above the embryo, the air space being separated from the external atmosphere by a partially gas permeable film, said incubating continuing until said embryo hatches.
Claim 2
Dependent Claim
167 words
A process for the in vitro culture of an avian embryo up to blastoderm formation, during embryonic morphogenesis and during embryonic growth to hatch, the process comprising culturing an avian embryo by a process comprising culturing a fertilized avian ovum, having a surrounding capsule of dense albumen, partially subemreged in culture medium in a first container sealed with a film having low gas permeability and not naturally associated with an resultant avian egg, up to blastoderm formation, subsequently culturing the avian embryo by a process comprising incubating said avian embryo during embryonic mrophogenesis in a culture medium in a liquid-filled liquid-impermeable second container sealed with a partially gas permeable film, and subsequently culturing the resultant avian embryo by a process comprising incubating said avian embryo during the embryonic growth phase in a closed second or third container, there being an air space above the embryo, the air space being separated from the external atmosphere by a partially gas permeable film, said incubating continuing until said embryo hatches.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-04-29 | 1994-10-29 | 1995-04-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-04-29 | 1998-10-29 | 1999-04-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-04-29 | 2002-10-29 | 2003-04-30 | $7700.00 | Window Closed / Lapsed |