US5008196A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stimulation of endothelial cell growth
Assignee: Genentech Inc
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Filing: 1987-08-21
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Granted: 1991-04-16
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Expires: 2007-08-21
Abstract
Abstract A method of stimulating endothelial cell growth is provided which comprises subjecting said cells to a growth stimulating amount of a highly purified vascular permeability factor having the following characteristics: (a) it has a Mr about 34,000-40,000 as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis; (b) it is a disulfide-linked protein dimer; (c) it has a N-terminal amino acid sequence as follows: <IMAGE> <IMAGE> <IMAGE> (d) it exhibits substantial mitogenic activity to endothelial cells in culture.
Inventors
- 👤 Daniel T. Connolly
- 👤 Joseph Feder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 15:11
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
78 words
The method of stimulating endothelial cell growth in vitro comprising subjecting said cells to a growth stimulating amount of a highly purified vascular permeability factor having the following characteristics of guinea pig VPF: (a) it has a M r about 34,000-40,000 as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis; (b) it is a disulfide-linked protein dimer; (c) it has a N-terminal amino acid sequence as follows: ##STR6## (d) it exhibits substantial mitogenic activity to endothelial cells in culture.
Claim 2
Independent Claim
78 words
The method of stimulating endothelial cell growth in vitro comprising subjecting said cells to a growth stimulating amount of a highly purified vascular permeability factor having the following characteristics of guinea pig VPF: (a) it has a M r about 34,000-40,000 as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis; (b) it is a disulfide-linked protein dimer; (c) it has a N-terminal amino acid sequence as follows: ##STR6## (d) it exhibits substantial mitogenic activity to endothelial cells in culture.
References Cited (Backward Citations)
US4456550A
Cited
US4721672A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-04-15 | 1994-10-15 | 1995-04-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-04-15 | 1998-10-15 | 1999-04-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-04-15 | 2002-10-15 | 2003-04-16 | $7700.00 | Window Closed / Lapsed |