US4994385A
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Heterobifunctional coupling agents
Assignee: Abbott Laboratories
•
Filing: 1988-09-22
•
Granted: 1991-02-19
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Expires: 2008-09-22
Abstract
Abstract Novel polyamino acid based coupling agents are disclosed. These reagents are useful for conjugating proteins (e.g. antibodies to enzymes) for use in diagnostic assays.
Inventors
- 👤 Christopher Bieniarz
- 👤 Christopher J. Welch
- 👤 Grady Barnes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 21:57
CPC Classifications
C; C07; C07D; C07D207/00; C07D207/02; C07D207/44; C07D207/444; C07D207/448; C07D207/452; C07K; C07K17/00; C07K17/02; C07K17/06; C07D207/46; C12; C12N; C12N11/00; C12N11/02; C12N11/06; Y; Y10; Y10S; Y10S435/00; Y10S435/964
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
76 words
A conjugate for use in a diagnostic assay, comprising: ##STR17## wherein B is an enzyme label, an antibody, or an antibody fragment; Q is an antibody or an antibody fragment when B is an enzyme label, or an enzyme label when B is an antibod or antibody fragment; X is an amino acid having from three to ten carbon atoms in a straight chain; R is cycloalkyl or alkyl-cycloalkyl; and n is from three to ten.
Claim 2
Dependent Claim
76 words
A conjugate for use in a diagnostic assay, comprising: ##STR17## wherein B is an enzyme label, an antibody, or an antibody fragment; Q is an antibody or an antibody fragment when B is an enzyme label, or an enzyme label when B is an antibod or antibody fragment; X is an amino acid having from three to ten carbon atoms in a straight chain; R is cycloalkyl or alkyl-cycloalkyl; and n is from three to ten.
Claim 3
Dependent Claim
11 words
The conjugate as recited in claim 1 wherein R is cyclohexylmethyl.
Claim 4
Dependent Claim
12 words
The conjugate of claim 1 wherein n is from three to five.
Claim 5
Dependent Claim
9 words
The conjugate of claim 3 wherein n is three.
Claim 6
Dependent Claim
17 words
The conjugate of claim 1 wherein X is a polyamide formed from repeated units of 6-aminocaproic acid.
Claim 7
Dependent Claim
15 words
The conjugate as recited in claim 1 wherein B is alkaline phosphatase or horseradish peroxidase.
Claim 8
Dependent Claim
12 words
The conjugate as recited in claim 1 wherein B is an antibody.
Claim 9
Dependent Claim
12 words
The conjugate as recited in claim 7 wherein B is goat-anti-rabbit IgG.
Claim 10
Dependent Claim
12 words
The conjugate as recited in claim 1 wherein Q is an antibody.
Claim 11
Dependent Claim
21 words
The conjugate as recited in claim 9 wherein Q is anti-AFP antibody, anti-CEA antibody, anti TSH antibody, or bovine gamma globulin.
Claim 12
Dependent Claim
12 words
The conjugate as recited in claim 1 wherein Q is an enzyme.
Claim 13
Dependent Claim
14 words
The conjugate as recited in claim 10 wherein Q is β-galactosidase, or alkaline phosphatase.
Claim 14
Dependent Claim
79 words
A conjugate for use in a diagnostic assay comprising: ##STR18## wherein X is a substituted or unsubstituted amino acid having from three to ten carbon atoms in a straight chain; R is an alkyl, cycloalkyl, alkyl-cycloalkyl, or an aromatic carbocyclic ring; n is from three to ten; Q is an enzyme or antibody; B is an enzyme when Q is an antibody, and B is an antibody when Q is an enzyme; and r is from two to fifty.
Claim 15
Dependent Claim
79 words
A conjugate for use in a diagnostic assay comprising: ##STR18## wherein X is a substituted or unsubstituted amino acid having from three to ten carbon atoms in a straight chain; R is an alkyl, cycloalkyl, alkyl-cycloalkyl, or an aromatic carbocyclic ring; n is from three to ten; Q is an enzyme or antibody; B is an enzyme when Q is an antibody, and B is an antibody when Q is an enzyme; and r is from two to fifty.
Claim 16
Dependent Claim
12 words
The conjugate as recited in claim 13 wherein B is an enzyme.
Claim 17
Dependent Claim
12 words
The conjugate as recited in claim 14 wherein B is alkaline phosphatase.
Claim 18
Dependent Claim
12 words
The conjugate as recited in claim 15 wherein Q is anti-AFP IgG.
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