US4994371A
Expired / Lapsed
US
⏱️ 0.0 years remaining
DNA preparation of Christmas factor and use of DNA sequences
Assignee: Individual
•
Filing: 1989-05-19
•
Granted: 1991-02-19
•
Expires: 2009-05-19
Abstract
Abstract There is disclosed an isolated DNA sequence and the amino acid sequence for human factor IX. The isolated DNA sequence and its flanking sequences are useful for determining mutations, deletions or other modifications in genetic sequences expressing normal factor IX or modifications thereof.
Inventors
- 👤 Earl W. Davie
- 👤 Kotoku Kurachi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 01:17
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/644; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Y; C12Y304/00; C12Y304/21; C12Y304/21022
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
13 words
An isolated DNA sequence consisting essentially of a sequence encoding human factor IX.
Claim 2
Dependent Claim
13 words
An isolated DNA sequence consisting essentially of a sequence encoding human factor IX.
Claim 3
Dependent Claim
12 words
The isolated DNA sequence of claim 1 wherein said DNA is cDNA.
Claim 4
Dependent Claim
14 words
The isolated DNA sequence of claim 1 where said DNA is closed chromosomal DNA.
Claim 5
Dependent Claim
21 words
An isolated DNA sequence encoding the amino acid sequence of the FIGURE from amino acid +1(Tyr) through amino acid +416 (Thr).
Claim 6
Dependent Claim
21 words
An isolated DNA sequence encoding the amino acid sequence of the FIGURE from amino acid +1(Tyr) through amino acid +416 (Thr).
Claim 7
Dependent Claim
22 words
An isolated DNA sequence encoding the amino acid sequence of the FIGURE from amino acid -46 (Met) to amino acid +416 (Thr).
Claim 8
Dependent Claim
22 words
An isolated DNA sequence encoding the amino acid sequence of the FIGURE from amino acid -46 (Met) to amino acid +416 (Thr).
Claim 9
Dependent Claim
24 words
An isolated DNA sequence encoding the sequence of the FIGURE from base 150 to base 1397, wherein said sequence codes for human factor IX.
Claim 10
Dependent Claim
24 words
An isolated DNA sequence encoding the sequence of the FIGURE from base 150 to base 1397, wherein said sequence codes for human factor IX.
Claim 11
Dependent Claim
27 words
An isolated DNA sequence encoding the sequence of the FIGURE from base 12 to base 1397, wherein said sequence codes for the precursor of human factor IX.
Claim 12
Dependent Claim
27 words
An isolated DNA sequence encoding the sequence of the FIGURE from base 12 to base 1397, wherein said sequence codes for the precursor of human factor IX.
Claim 13
Dependent Claim
59 words
A polynucleotide having a length of at least 14 nucleotides and not more than 3,000 nucleotides, wherein said polynucleotide is the same sequence as or complementary to a DNA base sequence encoding human factor IX, wherein said polynucleotide is free of other polynucleotide sequences of human origin that do not exist in the DNA sequence encoding human factor IX.
Claim 14
Dependent Claim
59 words
A polynucleotide having a length of at least 14 nucleotides and not more than 3,000 nucleotides, wherein said polynucleotide is the same sequence as or complementary to a DNA base sequence encoding human factor IX, wherein said polynucleotide is free of other polynucleotide sequences of human origin that do not exist in the DNA sequence encoding human factor IX.
Claim 15
Dependent Claim
22 words
A polynucleotide according to claim 8 wherein the polynucleotide is joined to a label moiety to provide a means for its direction.
Claim 16
Dependent Claim
12 words
A polynucleotide according to claim 9 wherein the label is a radionuclide.
Claim 17
Independent Claim
126 words
A method of detection in a human cell or cells, of a mutation in the gene encoding factor IX or the 5' flanking sequence of up to 1500 bases thereof, said method comprising: combining DNA from said human cell or cells with a polynucleotide having a length of at least 14 nucleotides and not more than 3,000 nucleotides, wherein said polynucleotide is the same sequence as, or is complementary to a DNA sequence encoding human factor IX, under hybridizing conditions of predetermined stringency; wherein said polynucleotide is free of other polynucleotide sequence of human origin that do not exist in the DNA sequence encoding human factor IX; washing the combined DNA and said polynucleotide; and detecting duplex formation as diagnostic of the presence of genetic mutation.
Claim 18
Independent Claim
126 words
A method of detection in a human cell or cells, of a mutation in the gene encoding factor IX or the 5' flanking sequence of up to 1500 bases thereof, said method comprising: combining DNA from said human cell or cells with a polynucleotide having a length of at least 14 nucleotides and not more than 3,000 nucleotides, wherein said polynucleotide is the same sequence as, or is complementary to a DNA sequence encoding human factor IX, under hybridizing conditions of predetermined stringency; wherein said polynucleotide is free of other polynucleotide sequence of human origin that do not exist in the DNA sequence encoding human factor IX; washing the combined DNA and said polynucleotide; and detecting duplex formation as diagnostic of the presence of genetic mutation.
Claim 19
Dependent Claim
22 words
A method according to claim 11, wherein said polynucleotide is joined to a label moiety to provide a means for its detection.
Claim 20
Dependent Claim
13 words
A method according to claim 12, wherein said label moiety is a radionuclide.
Claim 21
Dependent Claim
20 words
A method according to claim 13, wherein said DNA from said cell or cells is bound to a solid support.
Claim 22
Dependent Claim
12 words
A plasmid cloning vector comprising a DNA sequence encoding human factor IX.
Claim 23
Dependent Claim
12 words
A plasmid cloning vector comprising a DNA sequence encoding human factor IX.
Claim 24
Dependent Claim
13 words
The plasmid cloning vector of claim 15 wherein said DNA sequence comprises cDNA.
Claim 25
Dependent Claim
15 words
The plasmid cloning vector of claim 15 wherein said DNA sequence comprises cloned chromosomal DNA.
Claim 26
Dependent Claim
27 words
The plasmid cloning vector of claim 15, wherein said DNA sequence encodes the amino acid sequence of the FIGURE from amino acid +1(Tyr) to amino acid +416(Thr).
Claim 27
Dependent Claim
27 words
The plasmid cloning vector of claim 15 wherein said DNA sequence encodes the amino acid sequence of the FIGURE from amino acid (-36(Met) to amino acid +146(Thr).
Claim 28
Dependent Claim
32 words
The plasmid cloning vector of claim 15 wherein said DNA sequence comprises the nucleotide sequence of the FIGURE from base 150 to base 1397, which nucleotide sequence codes for human factor IX.
Claim 29
Dependent Claim
34 words
The plasmid cloning vector of claim 15 wherein said DNA sequence comprises the nucleotide sequence of the FIGURE from base 12 to base 1397, which sequence codes for the precursor of human factor IX.
Claim 30
Dependent Claim
18 words
The plasmid cloning vector of claim 15 further comprising a transcriptional promoter operably linked to the DNA sequence.
Claim 31
Dependent Claim
20 words
An isolated DNA sequence consisting essentially of a sequence encoding a human factor IX immediately preceded by a leader sequence.
Claim 32
Dependent Claim
20 words
An isolated DNA sequence consisting essentially of a sequence encoding a human factor IX immediately preceded by a leader sequence.
References Cited (Backward Citations)
US4497796A
Cited
GB2125409A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-02-18 | 1994-08-20 | 1995-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-02-18 | 1998-08-20 | 1999-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-02-18 | 2002-08-20 | 2003-02-19 | $7700.00 | Window Closed / Lapsed |