US4992382A
Expired / Lapsed
US
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Porous polymer film calcium ion chemical sensor and method of using the same
Assignee: Iowa State University Research Foundation Inc
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Filing: 1989-08-21
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Granted: 1991-02-12
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Expires: 2009-08-21
Abstract
Abstract A method of measuring calcium ions is disclosed wherein a calcium sensitive reagent, calcichrome, is immobilized on a porour polymer film. The reaction of the calcium sensitive reagent to the Ca(II) is then measured and concentration determined as a function of the reaction.
Inventors
- 👤 Marc D. Porter
- 👤 Lai-Kwan Chau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/84; G01N31/00; G01N31/22
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
93 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent having an acid selected from the group consisting of sulfonic acid and carboxylic acid and having calcium chelating groups on porous polymer film the acid capable of binding to the film and the calcium chelating groups retaining chelating properties after immobilization; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; and determining calcium concentration in said solution as a function of said reaction of the calcium sensitive reagent.
Claim 2
Independent Claim
93 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent having an acid selected from the group consisting of sulfonic acid and carboxylic acid and having calcium chelating groups on porous polymer film the acid capable of binding to the film and the calcium chelating groups retaining chelating properties after immobilization; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; and determining calcium concentration in said solution as a function of said reaction of the calcium sensitive reagent.
Claim 3
Dependent Claim
18 words
The method of claim 2 wherein said calcium sensitive reagent is immobilized on said film by electrostatic binding.
Claim 4
Dependent Claim
17 words
The method of claim 1 wherein said calcium sensitive reagent reaction is measured by diffuse reflectance spectroscopy.
Claim 5
Dependent Claim
33 words
The method of claim 1 wherein said reagent is immobilized on a film having a porosity to allow said calcium in said solution to permeate said film while prohibiting permeation of other macromolecules.
Claim 6
Dependent Claim
34 words
The method of claim 1 wherein said method is used for blood serum analysis and said reagent is immobilized on a film having a porosity of about 5,000 angstroms up to about 10 microns.
Claim 7
Dependent Claim
14 words
The method of claim 1 wherein calcichrome is used as said calcium sensitive reagent.
Claim 8
Dependent Claim
21 words
The method of claim 3 wherein said calcichrome is concentrated on said film at 5×10 -4 mM/g to 2×10 -2 mM/g.
Claim 9
Independent Claim
60 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent on a porous polymer film containing quarternized amine; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; and determining calcium concentration in said solution as a function of said reaction of the calcium sensitive reagent.
Claim 10
Independent Claim
60 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent on a porous polymer film containing quarternized amine; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; and determining calcium concentration in said solution as a function of said reaction of the calcium sensitive reagent.
Claim 11
Independent Claim
67 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent on porous polymer film; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; determining calcium concentration in said solution as a function of said reaction of said calcium sensitive reagent, wherein said calcium concentration may be determined in up to one minute.
Claim 12
Independent Claim
67 words
A method of measuring calcium in a solution comprising: immobilizing a calcium sensitive reagent on porous polymer film; contacting said film with said calcium containing solution; measuring reaction of said calcium sensitive reagent to said calcium containing solution; determining calcium concentration in said solution as a function of said reaction of said calcium sensitive reagent, wherein said calcium concentration may be determined in up to one minute.
Claim 13
Dependent Claim
59 words
A sensor for measuring the concentration of calcium in a solution comprising a porous polymer film having a calcium sensitive reagent immobilized thereon, the reagent comprising an acid selected from the group consisting of sulfonic acid and carboxylic acid capable of binding to said polymer film and having calcium chelating groups capable of retaining the chelating property after immobilization.
Claim 14
Dependent Claim
59 words
A sensor for measuring the concentration of calcium in a solution comprising a porous polymer film having a calcium sensitive reagent immobilized thereon, the reagent comprising an acid selected from the group consisting of sulfonic acid and carboxylic acid capable of binding to said polymer film and having calcium chelating groups capable of retaining the chelating property after immobilization.
Claim 15
Dependent Claim
28 words
The sensor of claim 10 wherein said film has a porosity to allow said calcium in said solution to permeate said film while prohibiting permeation of other macromolecules.
Claim 16
Dependent Claim
18 words
The sensor of claim 10 wherein said sensor measures said concentration of calcium in up to one minute.
Claim 17
Dependent Claim
20 words
The sensor of claim 10 wherein said film has a porosity of about 5,000 angstroms up to about 10 microns.
Claim 18
Dependent Claim
20 words
The sensor of claim 13 wherein said film has a porosity of about 0.5 microns up to about 10 microns.
Claim 19
Dependent Claim
12 words
The sensor of claim 10 wherein said calcium sensitive reagent is calcichrome.
Claim 20
Dependent Claim
21 words
The sensor of claim 15 wherein said film contains calcichrome at a concentration of 5×10 -4 mM/g to 2×10 -2 mM/g.
Claim 21
Dependent Claim
17 words
The sensor of claim 16 wherein said film contains calcichrome at a concentration of 1×10 -2 mM/g.
Claim 22
Dependent Claim
30 words
A sensor for measuring a concentration of calcium in a solution comprising a porous polymer film having a calcium sensitive reagent immobilized thereon, said porous polymer film containing quaternized amine.
Claim 23
Dependent Claim
30 words
A sensor for measuring a concentration of calcium in a solution comprising a porous polymer film having a calcium sensitive reagent immobilized thereon, said porous polymer film containing quaternized amine.
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