US4992274A
Expired / Lapsed
US
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Tissue plasminogen activator A-chain/urokinase B-chain hybrid protein
Assignee: Beecham Group PLC
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Filing: 1988-02-22
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Granted: 1991-02-12
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Expires: 2008-02-22
Abstract
Abstract A fibrinolytically active hybrid protein which comprises one chain of a 2-chain protease linked to a chain of a different 2-chain protease, or to the same chain of the same protease, at least one of the chains in the hybrid protein being derived from a fibrinolytically active protease, such that the hybrid protein has a catalytic site essential for fibrinolytic activity which is optionally blocked by a removable blocking group.
Inventors
- 👤 Jeffery H. Robinson
- 👤 Ian Dodd
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 16:04
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6435; C12N9/6456; C12N9/6459; C12Y; C12Y304/00; C12Y304/21; C12Y304/21007; C12Y304/21069; A; A61; A61K; A61K38/00; C07; C07K; C07K2319/00
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
26 words
A fibrinolytically active hybrid protein, which comprises tissue plasminogen activator A-chain covalently linked to urokinase B-chain and which has a catalytic site essential for catalytic activity.
Claim 2
Dependent Claim
26 words
A fibrinolytically active hybrid protein, which comprises tissue plasminogen activator A-chain covalently linked to urokinase B-chain and which has a catalytic site essential for catalytic activity.
Claim 3
Dependent Claim
36 words
The hybrid protein according to claim 1, wherein said hybrid protein comprises tissue plasminogen activator A-chain covalently linked to urokinase B-chain and has a catalytic site essential for catalytic activity blocked by a removable blocking group.
Claim 4
Dependent Claim
18 words
The hybrid protein according to claim 2, wherein said removable blocking group is benzoyl or a benzoyl derivative.
Claim 5
Dependent Claim
36 words
The hybrid protein according to claim 3, wherein said benzoyl derivative is benzoyl substituted by a moiety selected from the group consisting of halogen, C 1-6 alkyl, C 1-6 alkanoyloxy, C 1-6 alkanoylamino, amino and P-guanidino.
Claim 6
Dependent Claim
29 words
A pharmaceutical composition for treating thrombotic diseases, which comprises an effective amount of a fibrinolytically active hybrid protein according to claim 1, in combination with a pharmaceutically acceptable carrier.
Claim 7
Dependent Claim
31 words
A method of treating a patient suffering from a thrombotic disease, which comprises administering to the sufferer an effective non-toxic amount of a fibrinolytically active hybrid protein according to claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-02-11 | 1994-08-13 | 1995-02-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-02-11 | 1998-08-13 | 1999-02-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-02-11 | 2002-08-13 | 2003-02-12 | $7700.00 | Window Closed / Lapsed |