US4990452A
Expired / Lapsed
US
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Combining mutations for stabilization of subtilisin
Assignee: Genex Corp
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Filing: 1988-04-12
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Granted: 1991-02-05
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Expires: 2008-04-12
Abstract
Abstract The invention relates to modified subtilisin enzymes which have increased thermal stability. The modified subtilisin enzymes have at least two or more amino acid mutations which confer increased thermal stability. It has been discovered that combining individual stabilizing mutations in subtilisin frequently results in an additive increase in thermal stability. In addition, the invention pertains to cloned mutant genes coding for a subtilisin material having at least two amino acid substitution which has increased thermal stability.
Inventors
- 👤 Philip N. Bryan
- 👤 Michael W. Pantoliano
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 07:52
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K14/00; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/102; C12N15/11; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; C12P; C12P21/00; C12P21/02; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; C12Q1/68
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
54 words
A thermally stable subtilisin coded for by a mutant BPN' gene comprising the following amino acid substitutions: phenylalamine at amino acid position 50; alanine at amino acid position 169; cysteine at amino acid position 206; lysine at amino acid position 217; serine at amino acid position 218; and asparagine at amino acid position 76.
Claim 2
Dependent Claim
54 words
A thermally stable subtilisin coded for by a mutant BPN' gene comprising the following amino acid substitutions: phenylalamine at amino acid position 50; alanine at amino acid position 169; cysteine at amino acid position 206; lysine at amino acid position 217; serine at amino acid position 218; and asparagine at amino acid position 76.
Claim 3
Dependent Claim
9 words
A washing preparation comprising the subtilisin of claim 1.
References Cited (Backward Citations)
US4760025A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-02-04 | 1994-08-06 | 1995-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-02-04 | 1998-08-06 | 1999-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-02-04 | 2002-08-06 | 2003-02-05 | $7700.00 | Window Closed / Lapsed |