US4988302A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Culture media package
Assignee: Difco Laboratories Inc
•
Filing: 1989-06-08
•
Granted: 1991-01-29
•
Expires: 2009-06-08
Abstract
Abstract A culture media package comprising a container having a bottom wall and a peripheral side wall which is substantially filled with a culture medium, a removable insert over the top wall and having a portion defining a planar undersurface and a removable protective cover over the container and insert. The container and insert have radially extending flanges which are bonded to one another. The package is made by filling the container with a molten culture medium in a sterile environment, bonding the insert to the container in a sterile environment, inverting the sealed container such that the molten medium contacts the planar surface of the insert permitting any gases and bubbles to rise toward a space adjacent the bottom of the container and maintaining the container with the insert thereon in an inverted position until the medium has solidified. The removable cover may be applied to the sealed package of the container and insert either before the sealed package is inverted or after the medium has solidified. The package is preferably made from continuous molded plastic strips of connected containers and connected inserts which are filled with medium and sealed, severed from one another, thereafter inverted while the culture medium is still molten and permitted to remain in the inverted position until the culture medium has solidified.
Inventors
- 👤 Jerry W. Smith
- 👤 Nadine M. Sullivan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 18:05
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/38; C12M23/02; C12M23/10; C12M23/34; C12M37/00; C12M37/04
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
137 words
A sterile culture media package comprising a container having a bottom wall and a peripheral side wall, said container having a peripheral flange extending outwardly from the peripheral side wall, a removable insert comprising a bottomwall overlying said container and a peripheral flange extending outwardly from the peripheral side wall and overlying the peripheral flange of said container, said package being substantially filled with a culture medium in contact and solidified in situ on the surface of the bottom wall of the insert, said container including an integral portion defining a space adjacent the bottom wall into which gases and bubbles present after the package is filled and sealed have migrated, said peripheral flange of said container and said peripheral flange of said insert being in contact and sealed to one another to form a sterile package.
Claim 2
Dependent Claim
137 words
A sterile culture media package comprising a container having a bottom wall and a peripheral side wall, said container having a peripheral flange extending outwardly from the peripheral side wall, a removable insert comprising a bottomwall overlying said container and a peripheral flange extending outwardly from the peripheral side wall and overlying the peripheral flange of said container, said package being substantially filled with a culture medium in contact and solidified in situ on the surface of the bottom wall of the insert, said container including an integral portion defining a space adjacent the bottom wall into which gases and bubbles present after the package is filled and sealed have migrated, said peripheral flange of said container and said peripheral flange of said insert being in contact and sealed to one another to form a sterile package.
Claim 3
Dependent Claim
41 words
The culture media package set forth in claim 1 wherein said integral portion defining a space comprises a downwardly extending peripheral groove at the area of juncture of the bottom wall of the container with the peripheral wall of the container.
Claim 4
Dependent Claim
27 words
The culture media package set forth in claim 1 wherein said integral portion defining a space comprises an enlarged portion of the side wall of the container.
Claim 5
Dependent Claim
41 words
The culture media package set forth in claim 1 wherein said peripheral wall of said container tapers upwardly and outwardly from the base wall, said insert having a peripheral side wall extending axially inwardly toward the bottom wall of the container.
Claim 6
Dependent Claim
38 words
The culture media package set forth in claim 1 wherein said insert includes a portion defining a tab for engagement of the insert in order that the insert may be manually removed from its relationship to the container.
Claim 7
Dependent Claim
14 words
The culture media package set forth in claim 5 wherein said tab is flexible.
Claim 8
Dependent Claim
30 words
The culture media package set forth in claim 1 wherein said peripheral wall of said insert tapers upwardly and outwardly from the bottom wall toward the flange of the insert.
Claim 9
Dependent Claim
40 words
The culture media package set forth in claim 7 wherein said peripheral wall of said insert forms a greater angle with the axis of the container then the angle of the peripheral wall of the container makes with a vertical.
Claim 10
Dependent Claim
25 words
The culture media package set forth in claim 1 wherein said peripheral wall of said insert extends downwardly from the bottom wall of the insert.
Claim 11
Dependent Claim
31 words
The culture media package set forth in claim 9 wherein portions of said bottom wall of said insert are spaced axially from other portions to define different levels on the media.
Claim 12
Dependent Claim
25 words
The culture media package set forth in claim 10 wherein said container and insert define spaces, said media in said spaces differing from one another.
Claim 13
Dependent Claim
32 words
The culture media package set forth in any of claims 9-11 including a cover having spaced projections engaging the flange of said insert holding said cover in spaced relation to said insert.
Claim 14
Dependent Claim
73 words
The culture media package set forth in claim 1 wherein said container has a non-circular configuration in transverse cross section, said flanges of said container and said insert being sealed to one another along a complementary configuration but having portions extending circumferentially in unsealed relationship so that the peripheral edges of the flanges in the unsealed portions combine with the remainder of the configuration of the container and insert to form a circle.
Claim 15
Dependent Claim
31 words
The culture media package set forth in claim 13 including a cover having a base wall and a cylindrical peripheral wall telescoped over said flanges of said insert and said container.
Claim 16
Dependent Claim
34 words
The culture media package set forth in claim 1 wherein said peripheral flanges having portions in overlying and spaced relation such that portion of the insert can be grasped for removal of the insert.
Claim 17
Dependent Claim
16 words
The culture media package set forth in claim 1 wherein said insert comprises a flat sheet.
Claim 18
Dependent Claim
47 words
The culture media package set forth in claim 16 including a second container having a base wall and a peripheral wall, said first container package being telescoped in said second container and a cover having a top wall and a side wall telescoped onto said second container.
Claim 19
Dependent Claim
20 words
The culture media package set forth in claim 15 including means adhering said first mentioned container in said second container.
Claim 20
Dependent Claim
62 words
The culture media package set forth in claim 16 wherein said integral portion defining a space comprises a downwardly extending peripheral groove at the area of juncture of the bottom wall of the container with the peripheral wall of the container, said means for adhering comprises adhesive applied to the exterior of the bottom wall of the first container beneath said groove.
Claim 21
Dependent Claim
31 words
The culture media package set forth in any of claims 1-19 including a cover having a base wall and a peripheral flange telescoped over said flanges of said insert and container.
Claim 22
Dependent Claim
58 words
The culture media package set forth in claim 20 wherein said base of said cover includes a plurality of circumferentially spaced projections extending toward the insert such that when the insert is removed, said projections engage the flange of said container and maintain the base wall of the cover in spaced relationship to the surface of the media.
Claim 23
Dependent Claim
20 words
The culture media package set forth in any of claims 1-21 wherein said container and insert are made of plastic.
Claim 24
Independent Claim
163 words
The method of forming a sterile culture media package comprising forming a container having a bottom wall, a peripheral wall and an integral flange extending outwardly from said peripheral wall, forming an insert having a bottom wall overlying said container and a peripheral flange extending outwardly from said peripheral wall of said insert and overlying the peripheral flange of said container, filling the container with a culture medium to a predetermined level, applying the insert in overlying relationship to the container, moving the insert to bring the flange of the insert into contact with the flange of the container, sealing the flanges throughout the periphery thereof, inverting the sealed container and insert while the medium is molten to bring the culture medium into contact with the surface of the bottom wall of the insert, permitting the culture medium to solidify thereby forming the medium in situ on the under side of the bottom wall of the insert, and thereafter reinverting the resultant package.
Claim 25
Independent Claim
163 words
The method of forming a sterile culture media package comprising forming a container having a bottom wall, a peripheral wall and an integral flange extending outwardly from said peripheral wall, forming an insert having a bottom wall overlying said container and a peripheral flange extending outwardly from said peripheral wall of said insert and overlying the peripheral flange of said container, filling the container with a culture medium to a predetermined level, applying the insert in overlying relationship to the container, moving the insert to bring the flange of the insert into contact with the flange of the container, sealing the flanges throughout the periphery thereof, inverting the sealed container and insert while the medium is molten to bring the culture medium into contact with the surface of the bottom wall of the insert, permitting the culture medium to solidify thereby forming the medium in situ on the under side of the bottom wall of the insert, and thereafter reinverting the resultant package.
Claim 26
Dependent Claim
25 words
The method set forth in claim 23 including the step of evacuating and flushing the area between the insert and the medium in the container.
Claim 27
Dependent Claim
16 words
The method set forth in claim 23 wherein said step of sealing is by heat bonding.
Claim 28
Dependent Claim
16 words
The method set forth in claim 23 wherein said steps are performed in a sterile environment.
Claim 29
Dependent Claim
26 words
The method set forth in claim 23 including the step of stacking said packages in a vertical array and enclosing them in a container for handling.
Claim 30
Dependent Claim
29 words
The method set forth in claim 23 wherein said step of inverting said sealed container and insert comprises inverting said container at an angle between about 90° to 180°.
Claim 31
Dependent Claim
20 words
The method set forth in claim 23 wherein said step of inverting said container comprises inverting said container about 180°.
Claim 32
Dependent Claim
44 words
The culture media package set forth in any of claims 23-29 including the step of forming an integral portion in said container defining a space adjacent the bottom wall into which gases and bubbles present after the package is filled and sealed may migrate.
Claim 33
Independent Claim
42 words
The method set forth in any of claims 23-29 including the step of forming an integral portion in said container defining a space adjacent the side wall into which gases and bubbles present after the package is filled and sealed may migrate.
Claim 34
Independent Claim
30 words
The method set forth in any of claims 23-29 including the step of forming said peripheral wall insert such that it extends downwardly from the bottom wall of said insert.
Claim 35
Independent Claim
30 words
The method set forth in any of claims 23-29 including the step of telescoping said first-mentioned container into a second container and adhering said first-mentioned container to said second container.
Claim 36
Independent Claim
55 words
The method set forth in any of claims 23-29 including providing a cover and forming a plurality of spaced projections on said cover such that when the insert in removed, the projections engage the flange of the container and maintain the base wall of the cover in spaced relationship to the surface of the media.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-01-28 | 1994-07-30 | 1995-01-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-01-28 | 1998-07-30 | 1999-01-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-01-28 | 2002-07-30 | 2003-01-29 | $7700.00 | Window Closed / Lapsed |