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US4981985A
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Synthesis of photolabile chelators for multivalent cations
Assignee: TRUSTEES OF UNIVERSITY OF PENNSYLVANIA PHILADELPHIA PA A NON-PROFIT CORP OF
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Filing: 1987-10-20
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Granted: 1991-01-01
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Expires: 2007-10-20
Abstract
Abstract A method of synthesizing photolabile chelators as EDTA and EGTA derivatives to be used in caging multivalent cations is disclosed. The chelators chelate the cations forming non-biologically active compounds. The chelated compounds can then be localized in or near biological systems. Upon irradiation, the chelated compound cleaves with the subsequent cleaved remainders having a substantially lower affinity for the chelated cation. Large amounts of cation are thus rapidly released and the effect of such concentration jumps on the biological system can be accurately studied.
Inventors
- 👤 Jack H. Kaplan
- 👤 Graham C. R. Ellis-Davis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 16:45
CPC Classifications
C; C07; C07F; C07F3/00; C07F3/003; C07C; C07C229/00; C07C229/02; C07C229/04; C07C229/06; C07C229/10; C07C229/16
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
45 words
A solution comprising photolabile chelator consisting essentially of one or more anions having the following structure: ##STR11## wherein R 1 and R 2 are each chosen from the group consisting of hydrogen, methoxy, halogen, methyl, hydroxy, carboxy, benzyloxy, carboxymethyl, vinyl, nitro, amino, dimethylamine, and thioalkyl.
Claim 2
Dependent Claim
45 words
A solution comprising photolabile chelator consisting essentially of one or more anions having the following structure: ##STR11## wherein R 1 and R 2 are each chosen from the group consisting of hydrogen, methoxy, halogen, methyl, hydroxy, carboxy, benzyloxy, carboxymethyl, vinyl, nitro, amino, dimethylamine, and thioalkyl.
Claim 3
Dependent Claim
15 words
A chelated compound comprising the photolabile chelator of claim 1 chelated with a multivalent cation.
Claim 4
Dependent Claim
24 words
The chelated compound of claim 2 wherein the multivalent cation is chosen from the group consisting of calcium, magnesium, zinc, cadmium, manganese, or iron.
Claim 5
Dependent Claim
29 words
The chelated compound of claim 2 wherein light in the range of 250-500 NM causes the photolabile chelator to split and form compounds having the following two structures: ##STR12##
Claim 6
Dependent Claim
40 words
A photolabile chelator consisting essentially of anion having the following structure: ##STR13## wherein R 1 and R 2 are each chosen from the group consisting of hydrogen, methoxy, halogen, methyl, hydroxy, carboxy, benzyloxy, carboxymethyl, vinyl, nitro, amino, dimethylamine, and thioalkyl.
Claim 7
Dependent Claim
40 words
A photolabile chelator consisting essentially of anion having the following structure: ##STR13## wherein R 1 and R 2 are each chosen from the group consisting of hydrogen, methoxy, halogen, methyl, hydroxy, carboxy, benzyloxy, carboxymethyl, vinyl, nitro, amino, dimethylamine, and thioalkyl.
Claim 8
Dependent Claim
15 words
A chelated compound comprising the photolabile chelator of claim 5 chelated with a multivalent cation.
Claim 9
Dependent Claim
24 words
The chelated compound of claim 6 wherein the multivalent cation is chosen from the group consisting of calcium, magnesium, zinc, cadmium, manganese, or iron.
Claim 10
Dependent Claim
36 words
The chelated compound of claim 6 wherein light in the range of 250-500 NM causes the photolabile chelator to split and form compounds comprising the following two structures: ##STR14## with a resultant decrease in cation affinity.
References Cited (Backward Citations)
US4689432A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-31 | 1994-07-02 | 1995-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-31 | 1998-07-02 | 1999-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-31 | 2002-07-02 | 2003-01-01 | $7700.00 | Window Closed / Lapsed |