Patent US4980277A successfully refreshed from Google Patents.
US4980277A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cryoprotectant solution and method
Assignee: Danisco Finland Oy
•
Filing: 1987-10-16
•
Granted: 1990-12-25
•
Expires: 2007-10-16
Abstract
Abstract This invention relates to an improved cryoprotectant solution for viable cells, such as sperm, ova and blood cells which contains an effective amount of betaine.
Inventors
- 👤 Matti A. J. Junnila
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 21:31
CPC Classifications
A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/125
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
36 words
A cryoprotectant solution for viable cells which comprises an aqueous suspending vehicle which is physiologically acceptable to the cells and contains cryoprotectant additives and betaine in an amount of about 25% by weight of said solution.
Claim 2
Dependent Claim
36 words
A cryoprotectant solution for viable cells which comprises an aqueous suspending vehicle which is physiologically acceptable to the cells and contains cryoprotectant additives and betaine in an amount of about 25% by weight of said solution.
Claim 3
Dependent Claim
10 words
The cryoprotectant solution of claim 1 formulated for sperm cells.
Claim 4
Dependent Claim
47 words
A cryoprotectant solution for viable cells which is physiologically acceptable to the cells which comprises betaine in an amount of about 25% by weight of said solution and additionally contains lactose, egg yolk, glycerol, glucose, ethylene diamine tetracetic acid, sodium bicarbonate, trisodium citrate-2-hydrate, distilled water and penicillin.
Claim 5
Dependent Claim
47 words
A cryoprotectant solution for viable cells which is physiologically acceptable to the cells which comprises betaine in an amount of about 25% by weight of said solution and additionally contains lactose, egg yolk, glycerol, glucose, ethylene diamine tetracetic acid, sodium bicarbonate, trisodium citrate-2-hydrate, distilled water and penicillin.
Claim 6
Independent Claim
43 words
A method for cryopreservation of viable cells which comprises the steps of maintaining the cells at a cryogenic temperature in an aqueous suspending vehicle which is physiologically acceptable to the cells, said vehicle containing betaine in an amount of about 25% by weight.
Claim 7
Independent Claim
43 words
A method for cryopreservation of viable cells which comprises the steps of maintaining the cells at a cryogenic temperature in an aqueous suspending vehicle which is physiologically acceptable to the cells, said vehicle containing betaine in an amount of about 25% by weight.
Claim 8
Dependent Claim
13 words
The method as claimed in claim 4 wherein said viable cells are sperm.
Claim 9
Dependent Claim
13 words
The method as claimed in claim 5 wherein said sperm are stallion sperm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-24 | 1994-06-25 | 1994-12-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-24 | 1998-06-25 | 1998-12-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-24 | 2002-06-25 | 2002-12-25 | $7700.00 | Window Closed / Lapsed |