Patent US4977089A successfully refreshed from Google Patents.
US4977089A
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Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
Assignee: Eli Lilly and Co
•
Filing: 1987-01-30
•
Granted: 1990-12-11
•
Expires: 2007-01-30
Abstract
Abstract A novel promoter, ribosome-binding site-encoding DNA, and a number of signal peptide-encoding DNA sequences are described for use in Bacillus and other host cells. The compounds are useful in constructing expression vectors for use in Bacillus and other host cells and drive expression and secretion of proteins such as nuclease and human growth hormone. In addition, a general method for obtaining cells with increased abilities to express and secrete heterologous gene products is described.
Inventors
- 👤 Steven Kovacevic
- 👤 James R. Miller
- 👤 Loraine E. Veal
- 👤 John S. Wood
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:06
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C07K14/62; C12N9/00; C12N9/14; C12N9/16; C12N9/22; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/036; C07K2319/70; C07K2319/74; C07K2319/75
Claims Analysis
Total Claims: 26
Claim 1
Independent Claim
24 words
A recombinant DNA vector that comprises the DNA sequence ##STR11## wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytidyl, and T is thymidyl.
Claim 2
Dependent Claim
24 words
A recombinant DNA vector that comprises the DNA sequence ##STR11## wherein A is deoxyadenyl, G is deoxyguanyl, C is deoxycytidyl, and T is thymidyl.
Claim 3
Dependent Claim
164 words
A recombinant DNA vector that codes for expression of a gene product that contains the functional signal peptide: __________________________________________________________________________
MET THR
GLU TYR
LEU LEU SER ALA
GLY ILE
CYS
MET ALA
ILE
VAL
SER ILE
LEU LEU
ILE GLY MET ALA
ILE SER
ASN
VAL SER
LYS
GLY
GLN TYR
ALA LYS
ARG PHE PHE PHE
PHE ALA
THR
SER CYS
LEU
VAL
LEU THR
LEU VAL
VAL VAL SER SER
LEU SER
SER
SER ALA
ASN
ALA
__________________________________________________________________________ wherein ALA is alanine, ARG is arginine, ASN is asparagine, ASP is aspartic acid, CYS is cysteine, GLN is glutamine, GLU is glutamic acid, GLY is glycine, HIS is histidine, ILE is isoleucine, LEU is leucine, LYS is lysine, MET is methionine, PHE is phenylalanine, PRO is proline, SER is serine, THR is threonine, TRP is tryptophan, TYR is tyrosine, and VAL is valine, and wherein one or more of the residues from position 2 (threonine) up to position 28 (serine) have been deleted.
Claim 4
Dependent Claim
164 words
A recombinant DNA vector that codes for expression of a gene product that contains the functional signal peptide: __________________________________________________________________________
MET THR
GLU TYR
LEU LEU SER ALA
GLY ILE
CYS
MET ALA
ILE
VAL
SER ILE
LEU LEU
ILE GLY MET ALA
ILE SER
ASN
VAL SER
LYS
GLY
GLN TYR
ALA LYS
ARG PHE PHE PHE
PHE ALA
THR
SER CYS
LEU
VAL
LEU THR
LEU VAL
VAL VAL SER SER
LEU SER
SER
SER ALA
ASN
ALA
__________________________________________________________________________ wherein ALA is alanine, ARG is arginine, ASN is asparagine, ASP is aspartic acid, CYS is cysteine, GLN is glutamine, GLU is glutamic acid, GLY is glycine, HIS is histidine, ILE is isoleucine, LEU is leucine, LYS is lysine, MET is methionine, PHE is phenylalanine, PRO is proline, SER is serine, THR is threonine, TRP is tryptophan, TYR is tyrosine, and VAL is valine, and wherein one or more of the residues from position 2 (threonine) up to position 28 (serine) have been deleted.
Claim 5
Dependent Claim
139 words
The recombinant DNA vector of claim 2 that codes for expression of a gene product that contains the signal peptide: __________________________________________________________________________
MET ALA ILE
CYS
MET ALA
ILE
VAL
SER
ILE LEU LEU
ILE
GLY MET ALA
ILE
SER ASN
VAL
SER
LYS
GLY GLN TYR
ALA
LYS ARG PHE
PHE
PHE PHE
ALA
THR
SER
CYS LEU VAL
LEU
THR LEU VAL
VAL
VAL SER
SER
LEU
SER
SER SER ALA
ASN
ALA
__________________________________________________________________________
wherein ALA is alanine, ARG is arginine, ASN is asparagine, ASP is
aspartic acid, CYS is cysteine, GLN is glutamine, GLU is glutamic acid,
GLY is glycine, HIS is histidine, ILE is isoleucine, LEU is leucine, LYS
is lysine, MET is methionine, PHE is phenylalanine, PRO is proline, SER is
serien, THR is threonine, TRP is tryptophan, TYR is tyrosine, and VAL is
valine.
Claim 6
Dependent Claim
120 words
The recombinant DNA vector of claim 2 that codes for expression of a gene product that contains the signal peptide: __________________________________________________________________________
MET SER
LYS
GLY GLN TYR
ALA
LYS
ARG PHE
PHE
PHE
PHE ALA
THR
SER CYS LEU
VAL
LEU
THR LEU
VAL
VAL
VAL SER
SER
LEU SER SER
SER
ALA
ASN ALA
__________________________________________________________________________ wherein ALA is alanine, ARG is arginine, ASN is asparagine, ASP is aspartic acid, CYS is cysteine, GLN is glutamine, GLU is glutamic acid, GLY is glycine, HIS is histidine, ILE is isoleucine, LEU is leucine, LYS is lysine, MET is methionine, PHE is phenylalanine, PRO is proline, SER is serine, THR is threonine, TRP is tryptophan, TYR is tyrosine, and VBAL is valine.
Claim 7
Dependent Claim
11 words
The recombinant DNA vector of claim 1 that is a plasmid.
Claim 8
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW350.
Claim 9
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW352.
Claim 10
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW885.
Claim 11
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW350-16.
Claim 12
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW352-16.
Claim 13
Dependent Claim
9 words
The plasmid of claim 5 that is plasmid pOW885-16.
Claim 14
Dependent Claim
18 words
The plasmid of claim 5 that is selected from the group comprising pOW350, pOW352, pOW885, pOW350-16, pOW352-16, pOW885-16.
Claim 15
Dependent Claim
9 words
The vector of claim 5 that is plasmid pOW360.
Claim 16
Dependent Claim
9 words
The vector of claim 5 that is plasmid pOW360-16.
Claim 17
Dependent Claim
21 words
A recombinant host cell selected from the group consisting of E. coli and Bacillus transformed with a plasmid of claim 12.
Claim 18
Dependent Claim
21 words
A recombinant host cell selected from the group consisting of E. coli and Bacillus transformed with a vector of claim 1.
Claim 19
Dependent Claim
12 words
The recombinant host cell of claim 16 that is E. coli K12.
Claim 20
Dependent Claim
11 words
The recombinant host cell of claim 16 that is Bacillus subtilis.
Claim 21
Dependent Claim
13 words
The recombinant host cell of claim 15 that is E. coli K12 JA221/pOW350.
Claim 22
Dependent Claim
13 words
The recombinant host cell of claim 15 that is E. coli K12 JA221/pOW352.
Claim 23
Dependent Claim
13 words
The recombinant host cell of claim 15 that is E. coli K12 JA221/pOW885.
Claim 24
Dependent Claim
12 words
The recombinant host cell of claim 15 that is Bacillus subtilis MI112/pOW352-16.
Claim 25
Dependent Claim
12 words
The recombinant host cell of claim 15 that is Bacillus subtilis MI112/pOW885-16.
Claim 26
Dependent Claim
12 words
The recombinant host cell of claim 15 that is Bacillus subtilis MI112/pOW350-16.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-12-10 | 1994-06-11 | 1994-12-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-12-10 | 1998-06-11 | 1998-12-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-12-10 | 2002-06-11 | 2002-12-11 | $7700.00 | Window Closed / Lapsed |