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US4971908A
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Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
Assignee: Monsanto Technology LLC
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Filing: 1988-04-22
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Granted: 1990-11-20
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Expires: 2008-04-22
Abstract
Abstract Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthases, DNA encoding glyphosate-tolerant EPSP synthases, plant genes encoding the glyphosate-tolerant enzymes, plant transformation vectors containing the genes, transformed plant cells and differentiated transformed plants containing the plant genes are disclosed. The glyphosate-tolerant EPSP synthases are prepared by substituting an alanine residue for a glycine residue in a conserved sequence found between positions 80 and 120 in the mature wild-type EPSP synthase.
Inventors
- 👤 Ganesh M. Kishore
- 👤 Dilip M. Shah
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:05
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8261; C12N15/8271; C12N15/8274; C12N15/8275; C12N9/00; C12N9/10
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
52 words
A method for producing a gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which comprises altering a gene encoding EPSP synthase to cause the substitution of an alanine residue for the second glycine residue in the amino acid sequence: -L-G-N-A-G-T-A- located between positions 80 and 120 in a mature EPSP synthase sequence.
Claim 2
Independent Claim
52 words
A method for producing a gene encoding a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which comprises altering a gene encoding EPSP synthase to cause the substitution of an alanine residue for the second glycine residue in the amino acid sequence: -L-G-N-A-G-T-A- located between positions 80 and 120 in a mature EPSP synthase sequence.
Claim 3
Dependent Claim
20 words
A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a plant EPSP synthase gene.
Claim 4
Dependent Claim
20 words
A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a bacterial EPSP synthase gene.
Claim 5
Dependent Claim
21 words
A method of claim 1 in which the glyphosate-tolerant EPSP synthase gene is produced from a fungal gene encoding EPSP synthase.
Claim 6
Dependent Claim
24 words
A glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which contains the amino acid sequence: -L-G-N-A-A-T-A- between positions 80 and 120 in the mature EPSP synthase sequence.
Claim 7
Dependent Claim
24 words
A glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate (EPSP) synthase enzyme which contains the amino acid sequence: -L-G-N-A-A-T-A- between positions 80 and 120 in the mature EPSP synthase sequence.
Claim 8
Dependent Claim
15 words
The glyphosate-tolerant EPSP synthase enzyme of claim 5 as shown in FIGS. 1(a) and 1(b).
Claim 9
Dependent Claim
49 words
A glyphosate-tolerant EPSP synthase gene produced by the method of claim 1 wherein the wild-type EPSP synthase coding sequence is selected from the group of EPSP synthases consisting of Aspergillus nidulans, petunia, tomato, maize, Arabidopsis thaliana, Glycine max, E. coli K-12 and Salmonella typhimurium as shown in FIG. 2.
Claim 10
Dependent Claim
20 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Aspergillus nidulans.
Claim 11
Dependent Claim
19 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from petunia.
Claim 12
Dependent Claim
19 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from tomato.
Claim 13
Dependent Claim
19 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from maize.
Claim 14
Dependent Claim
20 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Arabidopsis thaliana.
Claim 15
Dependent Claim
20 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Glycine max.
Claim 16
Dependent Claim
21 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from E. coli K-12.
Claim 17
Dependent Claim
20 words
A glyphosate-tolerant EPSP synthase gene of claim 7 wherein the wild-type EPSP synthase coding sequence is selected from Salmonella typhimurium.
References Cited (Backward Citations)
US4769061A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-11-19 | 1994-05-21 | 1994-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-11-19 | 1998-05-21 | 1998-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-11-19 | 2002-05-21 | 2002-11-20 | $7700.00 | Window Closed / Lapsed |