US4963494A
Expired / Lapsed
US
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Enzyme immobilization in an anisotropic ultrafiltration membrane
Assignee: Nitto Denko Corp
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Filing: 1989-03-06
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Granted: 1990-10-16
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Expires: 2009-03-06
Abstract
Abstract An enzyme immobilized membrane comprising an anisotropic ultrafiltration membrane having a porous layer and a dense layer, and an enzyme immobilized therein is disclosed. The porous layer of the ultrafiltration membrane retains a water-soluble polymer having at least two functional groups in the crosslinked state, and the enzyme is covalently bonded to the membrane through the functional groups of the polymer. Preferably, the membrane is prepared from polysulfone. The enzyme immobilized membrane is produced by a process which comprises impregnating a solution of the water-soluble polymer into the porous layer of the ultrafiltration membrane under a pressure of 0.1 to 1.0 kg/cm 2 , washing the porous layer, passing a solution of a crosslinking agent through the porous layer under a pressure within the same pressure range as above to crosslink the water-soluble polymer, removing the non-crosslinked water-soluble polymer by back washing from the dense layer of the membrane, and then passing a solution of the enzyme under a pressure within the same pressure range as above through the porous layer, whereby the enzyme is covalently bonded to the membrane through the functional groups of the polymer.
Inventors
- 👤 Ken Hibino
- 👤 Takeshi Okada
- 👤 Kazuo Nakao
- 👤 Yuko Sahashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 07:38
CPC Classifications
B; B01; B01D; B01D67/00; B01D67/0081; B01D67/0088; B01D69/00; B01D69/14; B01D69/141; C; C12; C12N; C12N11/00; C12N11/02; C12N11/06; C12N11/08; C12N11/082; C12N11/089
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
84 words
An enzyme immobilized membrane comprising an anisotropic ultrafiltration polysulfone membrane including a dense layer having a molecular cut off from 1,000 to 1,000,000 and a porous layer, the porous layer having retained therein a water-soluble polymer with at least two functional groups in a crosslinked state, and an enzyme being covalently bonded to the functional groups whereby the enzyme is immobilized within the porous layer. wherein said water-soluble polymer is selected from the group consisting of a polyalkyleneimine, a polyaklylene glycol, and a polyallylamine.
Claim 2
Dependent Claim
84 words
An enzyme immobilized membrane comprising an anisotropic ultrafiltration polysulfone membrane including a dense layer having a molecular cut off from 1,000 to 1,000,000 and a porous layer, the porous layer having retained therein a water-soluble polymer with at least two functional groups in a crosslinked state, and an enzyme being covalently bonded to the functional groups whereby the enzyme is immobilized within the porous layer. wherein said water-soluble polymer is selected from the group consisting of a polyalkyleneimine, a polyaklylene glycol, and a polyallylamine.
Claim 3
Dependent Claim
16 words
The enzyme immobilized membrane as in claim 1, wherein the water-soluble polymer is polyethylenemine or polyallylamine.
Claim 4
Dependent Claim
20 words
The enzyme immobilized membrane as in claim 1, wherein the water-soluble polymer is cross-linked with a dialdehyde of a dissocyanate.
Claim 5
Dependent Claim
19 words
The enzyme immobilized membrane as in claim 1, wherein the enzyme is an enzyme for hydrolyzing polysaccharides or proteins.
Claim 6
Dependent Claim
172 words
A process for producing an enzyme immobilized membrane comprising an anisotropic ultrafiltration polysulfone membrane including a dense layer having a molecular cut-off of from 1,000 to 1,000,000 and a porous layer, and enzyme immobilized in the porous layer, which comprises impregnating a solution of a water-soluble polymer having at least two functional groups into the porous layer of said polysulfone membrane under a pressure of from 0.1 to 1.0 Kg/cm 2 , wherein said water-soluble polymer is selected from the group consisting of a polyalkyleneimine, a polyalkylene glycol, and a polyallylamine, washing the porous layer, passing a solution of a crosslinking agent through the porous layer under a pressure within said pressure range to crosslink the water-soluble polymer. removing a non-crosslinked portion of the water-soluble polymer by back washing from the dense layer of said polysulfone membrane, and passing a solution of an enzyme under said pressure through the porous layer to covalently bond the enzyme to said functional groups whereby said enzyme is immobilized within the porous layer of said membrane.
Claim 7
Dependent Claim
172 words
A process for producing an enzyme immobilized membrane comprising an anisotropic ultrafiltration polysulfone membrane including a dense layer having a molecular cut-off of from 1,000 to 1,000,000 and a porous layer, and enzyme immobilized in the porous layer, which comprises impregnating a solution of a water-soluble polymer having at least two functional groups into the porous layer of said polysulfone membrane under a pressure of from 0.1 to 1.0 Kg/cm 2 , wherein said water-soluble polymer is selected from the group consisting of a polyalkyleneimine, a polyalkylene glycol, and a polyallylamine, washing the porous layer, passing a solution of a crosslinking agent through the porous layer under a pressure within said pressure range to crosslink the water-soluble polymer. removing a non-crosslinked portion of the water-soluble polymer by back washing from the dense layer of said polysulfone membrane, and passing a solution of an enzyme under said pressure through the porous layer to covalently bond the enzyme to said functional groups whereby said enzyme is immobilized within the porous layer of said membrane.
Claim 8
Dependent Claim
14 words
The process as in claim 5, wherein the water-soluble polymer is polyethyleneimine or polyallylamine.
Claim 9
Dependent Claim
20 words
The process as in claim 5, wherein the concentration of the water-soluble polymer solution is 1% by weight or less.
Claim 10
Dependent Claim
33 words
The process as in claim 5, wherein, after impregnation, a pressure difference between said porous layer and said dense layer of said ultrafiltration polysulfone membrane is from 0.1 to 0.5 kg/cm 2 .
Claim 11
Dependent Claim
20 words
The process as in claim 5, wherein the crosslinking agent is selected from the group consisting of dialdehydes and diisocyanates.
Claim 12
Dependent Claim
32 words
The process as in claim 5, wherein the molar concentration ratio of the functional groups in the water-soluble polymer to the functional groups in the crosslinking agent is from 2 to 50.
Claim 13
Dependent Claim
17 words
The process as in claim 5, wherein the enzyme is an enzyme for hydrolyzing polysaccharides or proteins.
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| 3.5 Years (Year 4) | 1993-10-15 | 1994-04-16 | 1994-10-16 | $2000.00 | Window Closed / Lapsed |
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