EP0069869A1
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EP
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A membrane and an arrangement for the removal of a substance from a solution
Assignee: Gambro Lundia AB
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Filing: 1982-06-15
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Granted: —
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Expires: 2002-06-15
Abstract
Abstract An asymmetric, microporous membrane comprising an immobilized biologically active substance and an arrangement comprising such a membrane for the removal of a substance present in a solution. An arrangement comprises a casing (2) enclosing the asymmetric membrane (1, 1', 1 "), the solution containing the said substance being intended to be conducted from its source (7) through the casing (2) on the side of the barrier layer of the membrane to make diffusion contact with the biologically active substance enclosed in the pores of the membrane. The arrangement preferably comprises a further liquid cycle (11) comprising a pump (12) and a detector, communicating with the support layer side of the membrane inside the casing (2). The membrane is appropriately in the form of hollow fibres (1) with the biologically active substance (14) stabilized on a carrier (16) of such a size that it cannot penetrate wholly through the membrane pores (15) so as to avoid direct contact between the solution containing the said substance and the enclosed stabilized biologically active substance.
Inventors
- 👤 Ingvar Gösta Holger Sjöholm
- 👤 Peter Kjell Rudolf Edman
- 👤 Ulf Thomas Gustav Nylen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 20:33
CPC Classifications
B; B01; B01D; B01D61/00; B01D65/00; B01D65/10; B01D65/104; B01D69/00; B01D69/08; B01D69/081; C; C12; C12M; C12M25/00; C12M25/02; C12M25/10; C12M25/14; C12M25/16; C12M29/00; C12M29/02; C12M29/16; C12P; C12P13/00; C12P13/04; C12P13/20; C12P19/00; C12P19/14; C12P37/00; C12P37/06
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
60 words
A microporous, semipermeable, asymmetric membrane to be used in conjunction with the removal of a substance from a solution, this membrane comprising an immobilized biologically active substance (14) enclosed in the pores of the membranes to make diffusion contact with the said solution, characterized in that the biologically active substance (14) is immobilized on and/or in microparticulate gel grains (16).
Claim 2
Dependent Claim
60 words
A microporous, semipermeable, asymmetric membrane to be used in conjunction with the removal of a substance from a solution, this membrane comprising an immobilized biologically active substance (14) enclosed in the pores of the membranes to make diffusion contact with the said solution, characterized in that the biologically active substance (14) is immobilized on and/or in microparticulate gel grains (16).
Claim 3
Dependent Claim
43 words
A membrane in accordance with claim 1, characterized in that the said membrane (1) has pores (15) of increasing pore size in the direction from the side (19) of the membrane which is intended to face towards the solution containing the said substance.
Claim 4
Dependent Claim
39 words
A membrane in accordance with claim 1 or 2, characterized in that it is present in the form of hollow fibres, the solution containing the said substance being intended to pass through the axial cavity (18) of these fibres.
Claim 5
Dependent Claim
36 words
A membrane in accordance with claim 3, characterized in that the smallest pore size of the hollow fibres is less than the size of the enclosed microparticulate gel grains (16) carrying immobilized biologically active substance (14).
Claim 6
Dependent Claim
43 words
A membrane in accordance with claim 3 or 4, characterized in that the pore size on the side (17) of hollow fibres which is intended to be remote from the solution containing the said substance is > 1 µm, preferably > 10 µm.
Claim 7
Dependent Claim
27 words
A membrane in accordance with anyone of claims 3-5, characterized in that the hollow fibres have a cut-off of < 150,000 Daltons, preferably 20,000 - 50,000 Daltons.
Claim 8
Dependent Claim
29 words
A membrane in accordance with anyone of claims 1-6, characterized in that the said gel grains (16) have a diameter of < 10 µm, preferably 0.1 - 0.5 µm.
Claim 9
Dependent Claim
26 words
A membrane in accordance with anyone of claims 1-7, characterized in that the said gel grains (16) are selected among polyacrylamide, agarose and polystyrene, preferably polyacrylamide.
Claim 10
Dependent Claim
24 words
A membrane in accordance with anyone of claims 1-8, characterized in that it is selected among polyamide, cellulose acetate, polysulphone, polycarbonate, polyacrylonitrile, preferably polyamide.
Claim 11
Dependent Claim
48 words
A membrane. in accordance with anyone of the preceding claims, characterized d in that the biologically active substance is selected among antibodies, antigens, protein A, killed bacteria or fragments thereof and enzymes such as asparaginase, aspartase from E.coli, penicillanic acid from E.coli, β-galactosidase from E.coli and yeast fungus.
Claim 12
Dependent Claim
62 words
An arrangement for the removal of a substance from a solution, this arrangement comprising a microporous, semipermeable, asymmetric membrane (1, 1', 1"; 1a, 1'a, 1"a) with a biologically active substance (14) immobilized inside the pores of the membrane to make diffusion contact with the said solution, characterized in that the biologically active substance is immobilized on and/or in microparticulate gel grains (16).
Claim 13
Dependent Claim
62 words
An arrangement for the removal of a substance from a solution, this arrangement comprising a microporous, semipermeable, asymmetric membrane (1, 1', 1"; 1a, 1'a, 1"a) with a biologically active substance (14) immobilized inside the pores of the membrane to make diffusion contact with the said solution, characterized in that the biologically active substance is immobilized on and/or in microparticulate gel grains (16).
Claim 14
Dependent Claim
31 words
An arrangement in accordance with claim 12, characterized in that the said membrane (1) has pores (15) of increasing pore size in direction from the one side (19) of the membrane.
Claim 15
Dependent Claim
43 words
An arrangement in accordance with claim 11 or 12, character ized d in that the said membrane is present in the form of hollow fibres, the solution containing the said substance being intended to pass through the axial cavity (18) of these fibres.
Claim 16
Dependent Claim
42 words
An arrangement in accordance with claim 13, characterized in that the said hollow fibres are enclosed within an outer casing (2; 2a) with a first inlet (3; 3a) and a first outlet (4; 4a) communicating with the axial cavity of the fibres.
Claim 17
Dependent Claim
53 words
An arrangement in accordance with claim 14, characterized in that the said casing (2; 2a) comprises a second inlet (5; 5a) and a second outlet (6; 6a) respectively communicating with an outer liquid cycle for a liquid which is intended to pass through the said casing on the outside of the hollow fibres.
Claim 18
Dependent Claim
33 words
An arrangement in accordance with claim 15, characterized in that the said outer liquid cycle comprises a pump (12) preferably a so-called roller pump, for circulation of the said liquid in this cycle.
Claim 19
Dependent Claim
37 words
An arrangement in accordance with claim 15 or 16, characterized in that the said outer cycle comprises a detector (13) for the detecting of possible leakage of solution containing the said substance within the casing (2; 2a).
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