US4960694A
Expired / Lapsed
US
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Test combination and method of detecting fibrin monomers in blood
Assignee: Individual
•
Filing: 1987-07-09
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Granted: 1990-10-02
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Expires: 2007-07-09
Abstract
Abstract A test reagent for detecting fibrin monomers in blood, comprises an antibiotic of a pyrrole amidine series as a precipitant, introduced in a buffer solution. A method of detecting fibrin monomers in blood, comprises the steps of mixing venous blood with an anticoagulant, centrifuging the mixture of the venous blood with the anticoagulant, removing a citrate plasma, using an antibiotic of a pyrrole amidine series as a precipitant, and evaluating a precipitation reaction which takes place thereby.
Inventors
- 👤 Klaus Eckardt
- 👤 Gottfried Toepfer
- 👤 Andreas Seifert
- 👤 Manfred Schulze
- 👤 Udo Funke
- 👤 Marlena Stepanauskas, deceased
- 👤 legal representative by Leonas-Vitas Stepanauskas
- 👤 legal representative Daina Stepanauskas
- 👤 Heinz Thrum, deceased
- 👤 legal representative by Margot Thrum
- 👤 legal representative by Matthias Thrum
- 👤 legal representative by Michael Thrum
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 18:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
84 words
A method of detecting the presence of fibrin monomer in blood comprising the steps of: a. mixing venous blood with an anticoagulant to form a mixture, b. centrifuging said mixture, c. removing plasma from said mixture, d. mixing said plasma with a test reagent comprising an antibiotic of the pyrrole-amidine series dissolved in a buffer solution to from a reaction mixture, and e. evaluating said reaction mixture for the presence of a precipitate indicative of the presence of said fibrin monomer in said blood.
Claim 2
Independent Claim
84 words
A method of detecting the presence of fibrin monomer in blood comprising the steps of: a. mixing venous blood with an anticoagulant to form a mixture, b. centrifuging said mixture, c. removing plasma from said mixture, d. mixing said plasma with a test reagent comprising an antibiotic of the pyrrole-amidine series dissolved in a buffer solution to from a reaction mixture, and e. evaluating said reaction mixture for the presence of a precipitate indicative of the presence of said fibrin monomer in said blood.
Claim 3
Dependent Claim
29 words
The method of claim 1, further comprising before said step of evaluating, allowing said reaction mixture to stand for 30 minutes at room temperature and then centrifuging the same.
Claim 4
Dependent Claim
20 words
The method of claim 1, employing said antibiotic selected from the group consisting of netropsin, anthelvencin, distamycin and analogs thereof.
Claim 5
Dependent Claim
12 words
The method of claim 1, wherein said buffer is an HEPES-buffer solution.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-10-01 | 1994-04-02 | 1994-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-10-01 | 1998-04-02 | 1998-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-10-01 | 2002-04-02 | 2002-10-02 | $7700.00 | Window Closed / Lapsed |