US4710459A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process and reagent for the determination of fibrin monomer in plasma
Assignee: Roche Diagnostics GmbH
•
Filing: 1985-08-21
•
Granted: 1987-12-01
•
Expires: 2005-08-21
Abstract
Abstract The present invention provides a process for the determination of fibrin monomer in plasma, wherein a plasma sample containing fibrin monomer and freed from plasmin inhibitors is incubated in buffered solution with tissue plasminogen activator (EPA), plasminogen and a chromogenic plasmin substrate and the color formed is measured as a measure of the amount of fibrin monomer. The present invention also provides a reagent for the determination of fibrin monomer in plasma, wherein it contains plasminogen, tissue plasminogen activator (EPA), chromogenic plasmin substrate and buffer (pH 7.0 to 9.0).
Inventors
- 👤 Knut Bartl
- 👤 Helmut Lill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:56
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/56; C12Q2337/00; C12Q2337/10; C12Q2337/12; G; G01; G01N; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/972; G01N2333/9726; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
55 words
Process for the determination of fibrin monomer in plasma, comprising incubating a plasma sample containing fibrin monomer and freed from plasmin inhibitors in buffered solution with tissue plasminogen activator (EPA), plasminogen and a chromogenic plasmin substrate to form a color and measuring color formed to determine the amount of fibrin monomer present in said sample.
Claim 2
Dependent Claim
55 words
Process for the determination of fibrin monomer in plasma, comprising incubating a plasma sample containing fibrin monomer and freed from plasmin inhibitors in buffered solution with tissue plasminogen activator (EPA), plasminogen and a chromogenic plasmin substrate to form a color and measuring color formed to determine the amount of fibrin monomer present in said sample.
Claim 3
Dependent Claim
31 words
Process according to claim 1, wherein the plasma sample is obtained by mixing plasma with acid to form a precipitate, separating the precipitate formed and resuspending the precipitate in buffer solution.
Claim 4
Dependent Claim
11 words
Process according to claim 2, wherein said acid is acetic acid.
Claim 5
Dependent Claim
36 words
Process according to claim 1, wherein the plasma sample is obtained by incubating the plasma containing a fibrin monomer with a polystyrene body, to bind fibrin monomer to said body, and removing liquid from the sample.
Claim 6
Dependent Claim
32 words
Process according to claim 1, wherein the plasma sample is obtained by mixing the plasma containing a fibrin monomer with polystyrene latex and incubating to bind fibrin monomer to said polystyrene latex.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-30 | 1991-06-01 | 1991-12-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-30 | 1995-06-01 | 1995-12-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-30 | 1999-06-01 | 1999-12-01 | $7700.00 | Window Closed / Lapsed |