US4959179A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilized enzymes liquid detergent composition containing lipase and protease
Assignee: Henkel IP and Holding GmbH
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Filing: 1989-01-30
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Granted: 1990-09-25
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Expires: 2009-01-30
Abstract
Abstract The invention relates to the stabilization of mixtures of proteolytic and lipolytic enzymes in a liquid medium such as a liquid detergent composition. By inclusion therein of a stabilizing system comprising a polyol and a boron compound which are capable of reacting with each other whereby the polyol has a first binding constant with the boron compound of at least 500 l/mole and a second binding constant of at least 1,000 l 2 /mole 2 , the stability of the lipase in the presence of the protease is significantly improved. The lipase is preferably obtained from Humicola lanuginosa, and the stabilizing system preferably comprises a mixture of sorbitol and borax.
Inventors
- 👤 Michael P. Aronson
- 👤 Martin S. Cardinali
- 👤 Jack T. McCown
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:49
CPC Classifications
C; C11; C11D; C11D3/00; C11D3/16; C11D3/38; C11D3/386; C11D3/38663
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
152 words
An enzymatic liquid detergent and cleaning composition comprising, in a liquid medium, from 0-90% by weight of a detergent-active compound, a proteolytic enzyme and a lipolytic enzyme, said lipolytic enzyme being selected from the group consisting of fungal lipases obtainable from Humicola lanuginosa and Thermomyces lanuginosus, and bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by Chromobacter viscosum var. lipolyticum NRRL-B3673, and an enzyme stabilizing system comprising a mixture of a polyol containing only C, H and O atoms and containing at least two hydroxyl groups and a boron compound which is capable of reacting with said polyol, wherein said polyol has a first binding constant with said boron compound of at least 500 l/mole and a second binding constant of at least 1,000 l 2 /mole 2 , and wherein the weight ratio of said polyol to said boron compound is greater than 1.0.
Claim 2
Dependent Claim
152 words
An enzymatic liquid detergent and cleaning composition comprising, in a liquid medium, from 0-90% by weight of a detergent-active compound, a proteolytic enzyme and a lipolytic enzyme, said lipolytic enzyme being selected from the group consisting of fungal lipases obtainable from Humicola lanuginosa and Thermomyces lanuginosus, and bacterial lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by Chromobacter viscosum var. lipolyticum NRRL-B3673, and an enzyme stabilizing system comprising a mixture of a polyol containing only C, H and O atoms and containing at least two hydroxyl groups and a boron compound which is capable of reacting with said polyol, wherein said polyol has a first binding constant with said boron compound of at least 500 l/mole and a second binding constant of at least 1,000 l 2 /mole 2 , and wherein the weight ratio of said polyol to said boron compound is greater than 1.0.
Claim 3
Dependent Claim
26 words
The composition of claim 1, wherein the lipase is a lipase, obtained by cloning the gene from Humicola lanuginosa and expressing this gene in Aspergillus oryzae.
Claim 4
Dependent Claim
12 words
The composition of claim 1, wherein the polyol is sorbitol or 1,2-benzenediol.
Claim 5
Dependent Claim
12 words
The composition of claim 1, wherein the boron compound is sodium tetraborate.
Claim 6
Dependent Claim
14 words
The composition of claim 1, wherein the proteolytic enzyme is a bacterial subtilisin protease.
Claim 7
Dependent Claim
60 words
The composition of claim 1, comprising, in a liquid medium, from 5-90% by weight of the detergent-active compound, from 0.1-50 GU per milligramme of the final composition of the proteolytic enzyme, from 0.005-100 LU per milligramme of the final composition of the lipolytic enzyme, from 1-20% by weight of the polyol, and from 1-10% by weight of the boron compound.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-09-24 | 1994-03-26 | 1994-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-09-24 | 1998-03-26 | 1998-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-09-24 | 2002-03-26 | 2002-09-25 | $7700.00 | Window Closed / Lapsed |