US4946958A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chemiluminescent acridinium labelling compounds
Assignee: University College Cardiff Consultants Ltd
•
Filing: 1988-09-26
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Granted: 1990-08-07
•
Expires: 2008-09-26
Abstract
Abstract A compound for use as a chemiluminescent label in immunoassay comprises an aryl acridinium ester linked to an N-succinimidyl moiety. The compound is conveniently linked to a monoclonal antibody or other protein and is used in a two-site immunoassay for the quantitation of an antigen of interest, by initiation of the luminescent reaction and subsequent measurement of the photonic emission of the immune complex formed during the immunological reaction.
Inventors
- 👤 Anthony K. Campbell
- 👤 James S. Woodhead
- 👤 Ian Weeks
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:00
CPC Classifications
C; C07; C07D; C07D401/00; C07D401/02; C07D401/12; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S435/00; Y10S435/968; Y10S530/00; Y10S530/802
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
146 words
A chemiluminescent labelling compound for use in the labelling of substances of biological interest, said compound being capable of undergoing a light emitting reaction and defined by the formula: ##STR5## wherein X - is an anion, R 1 is a substituent selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl and aryl, R 2 and R 3 are substituents selected from the group consisting of hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro and halide, and R 4 is a substituted phenoxy moiety, with a reactive group capable of reacting with a substance of biological interest being attached, either directly or by means of an alkyl group, to the aryl portion of said phenoxy moiety, said reactive group being selected from the group consisting of: ##STR6## wherein R 6 has the same definition as R 1 , and Y is a halide, (e) --halide, and (f) --azide.
Claim 2
Dependent Claim
146 words
A chemiluminescent labelling compound for use in the labelling of substances of biological interest, said compound being capable of undergoing a light emitting reaction and defined by the formula: ##STR5## wherein X - is an anion, R 1 is a substituent selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl and aryl, R 2 and R 3 are substituents selected from the group consisting of hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro and halide, and R 4 is a substituted phenoxy moiety, with a reactive group capable of reacting with a substance of biological interest being attached, either directly or by means of an alkyl group, to the aryl portion of said phenoxy moiety, said reactive group being selected from the group consisting of: ##STR6## wherein R 6 has the same definition as R 1 , and Y is a halide, (e) --halide, and (f) --azide.
Claim 3
Dependent Claim
26 words
A compound according to claim 1, wherein said reactive group is attached to said aryl portion of said phenoxy moiety by means of an alkyl group.
Claim 4
Dependent Claim
16 words
A compound according to claim 2, wherein said alkyl group is --CH 2 CH 2 --.
Claim 5
Dependent Claim
21 words
A compound according to claim 1, wherein said reactive group is directly attached to said aryl portion of said phenoxy moiety.
Claim 6
Dependent Claim
16 words
A compound according to claim 1, wherein said reactive group is represented by the formula: ##STR7##
Claim 7
Dependent Claim
28 words
A luminescent labelling compound for use in the labelling of substances of biological interest, said compound being of the following formula: ##STR8## wherein X - is an anion.
Claim 8
Dependent Claim
28 words
A luminescent labelling compound for use in the labelling of substances of biological interest, said compound being of the following formula: ##STR8## wherein X - is an anion.
Claim 9
Dependent Claim
15 words
A compound according to claim 6, wherein X - is (CH 3 O)SO 3 .
References Cited (Backward Citations)
US3352791A
Cited
US3431264A
Cited
GB1316363A
Cited
US3689391A
Cited
US3817837A
Cited
GB1461877A
Cited
US4199559A
Cited
GB1548741A
Cited
US4478817A
Cited
GB2008247A
Cited
US4277437A
Cited
GB2025609A
Cited
GB2044449A
Cited
US4376110A
Cited
EP0082636A1
Cited
US4767718A
Cited
EP0212951A2
Cited
EP0216553A2
Cited
DE3628573A1
Cited
US4745181A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-08-06 | 1994-02-05 | 1994-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-08-06 | 1998-02-05 | 1998-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-08-06 | 2002-02-05 | 2002-08-07 | $7700.00 | Window Closed / Lapsed |