US4941101A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for analyzing chromatograms
Assignee: Agilent Technologies Inc
•
Filing: 1987-09-01
•
Granted: 1990-07-10
•
Expires: 2007-09-01
Abstract
Abstract A method for resolving severely overlapped chromatogram peaks using Jansson's iterative deconvolution method is disclosed. The method requires only a knowledge of the impulse response function of the chromatographic apparatus and maximum peak height. A high degree of resolution is achieved without significantly degrading the chromatogram signal to noise ratio.
Inventors
- 👤 Paul B. Crilly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 05:58
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/02; G01N30/86; G01N30/8624; G01N30/8631
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
234 words
Method of resolving fused peaks present in a chromatogram provided by a chromatographic apparatus, wherein the fused peaks are representative of at least two components of a sample that has been injected into the chromatographic apparatus, comprising the steps of: (a) providing a first data set indicative of the chromatogram to the processing means; (b) applying, via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and wherein the peak resolution function is applied to the first data set according to: x.sup.(k+1) (t)=x.sup.(k) (t)+r [x.sup.(k) (t)](g(t)-h(t)*x.sup.(k) t)) where: x.sup.(k) (t) is the k'th estimate of x(t); r[x.sup.(k) (t)] is the relaxation function; g(t) is the first data set; h(t) is an impulse response function of the chromatographic apparatus; and, (c) providing a second data set representative of the result of the iterations of the peak resolution function; and, (d) determining an appropriate retention time for the sample; (e) selecting a pure compound having a retention time approximating the retention time of the sample; (f) injecting the pure compound into the chromatographic apparatus; (g) producing a chromatogram indicative of the impulse response function for the chromatographic apparatus; and (h) providing an impulse response function data set indicative of the impulse response function chromatogram to the processing means.
Claim 2
Dependent Claim
234 words
Method of resolving fused peaks present in a chromatogram provided by a chromatographic apparatus, wherein the fused peaks are representative of at least two components of a sample that has been injected into the chromatographic apparatus, comprising the steps of: (a) providing a first data set indicative of the chromatogram to the processing means; (b) applying, via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and wherein the peak resolution function is applied to the first data set according to: x.sup.(k+1) (t)=x.sup.(k) (t)+r [x.sup.(k) (t)](g(t)-h(t)*x.sup.(k) t)) where: x.sup.(k) (t) is the k'th estimate of x(t); r[x.sup.(k) (t)] is the relaxation function; g(t) is the first data set; h(t) is an impulse response function of the chromatographic apparatus; and, (c) providing a second data set representative of the result of the iterations of the peak resolution function; and, (d) determining an appropriate retention time for the sample; (e) selecting a pure compound having a retention time approximating the retention time of the sample; (f) injecting the pure compound into the chromatographic apparatus; (g) producing a chromatogram indicative of the impulse response function for the chromatographic apparatus; and (h) providing an impulse response function data set indicative of the impulse response function chromatogram to the processing means.
Claim 3
Dependent Claim
24 words
Method according to claim 1 further comprising the step of plotting the second data set to provide a graphical representatiave of amplitude versus time.
Claim 4
Dependent Claim
34 words
Method of claim 1 wherein the relaxation function is determined according to: r[x.sup.(k) (t)]=b[(1-2/c) |x.sup.(k) (t)-c/2|] where: b is a relaxation constant; and c is the maximum peak height of the first data set.
Claim 5
Dependent Claim
146 words
Method of resolving fused peaks present in a chromatogram provided by a chromatographic apparatus, wherein the fused peaks are representative of at least two components of a sample that has been injected into the chromatographic apparatus, comprising the steps of: (a) providing a first data set indicative of the chromatogram to processing means; (b) applying via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and (c) providing a second data set representative of the result of the iterations of the peak resolution function; (d) analyzing the first data set to determine the severity of fusing peaks of the chromatogram; and (e) selecting the number of iterations based on the severity of fusing of peaks of the chromatogram.
Claim 6
Dependent Claim
146 words
Method of resolving fused peaks present in a chromatogram provided by a chromatographic apparatus, wherein the fused peaks are representative of at least two components of a sample that has been injected into the chromatographic apparatus, comprising the steps of: (a) providing a first data set indicative of the chromatogram to processing means; (b) applying via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and (c) providing a second data set representative of the result of the iterations of the peak resolution function; (d) analyzing the first data set to determine the severity of fusing peaks of the chromatogram; and (e) selecting the number of iterations based on the severity of fusing of peaks of the chromatogram.
Claim 7
Dependent Claim
26 words
Method of claim 4 further comprising the step of preparing the first data set prior to applying the peak resolution function to resolve the fused peaks.
Claim 8
Dependent Claim
43 words
Method of claim 5 wherein the step of preparing the first data set further comprises the steps of: (a) selecting a portion of the first data set for interpretation; (b) removing baseline offset from the selected portion; and (c) normalizing the selected portion.
Claim 9
Dependent Claim
44 words
Method of claim 6 wherein the selecting step further comprises the steps of: (a) determining a maximum peak value for the data set; (b) locating the maximum peak; and (c) stripping off all data from the data set not associated with the maximum peak.
Claim 10
Dependent Claim
21 words
Method of claim 6 further comprising the step of scaling the impulse response function with respect to the normalized selected portion.
Claim 11
Dependent Claim
18 words
Method of claim 6 further comprising the step of smoothing the selected portion and the impulse response function.
Claim 12
Dependent Claim
205 words
Method of resolving fused peaks present in a chromatogram comprising the steps of: (a) injecting a sample consisting of at least two components into a gas chromatographic apparatus having an injection port, a column and a detection port; (b) analyzing the sample to produce a gas chromatogram; (c) providing a first data set indicative of the chromatogram to processing means; (d) applying, via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and (e) providing a second data set representative of the result of the iterations of the peak resolution function; and, wherein the step of analyzing the sample to produce a gas chromatogram further comprises the steps of: (f) evaporating the same with a hot, inert gas; (g) transporting the evaporated sample from the injection port, through the column, to the detection port; and (h) detecting at least two components of the evaporated sample upon arrival at the detection port; and, wherein the step of transporting the evaporated sample further comprises the step of temporarily retaining each of the at least two components in the column.
Claim 13
Dependent Claim
205 words
Method of resolving fused peaks present in a chromatogram comprising the steps of: (a) injecting a sample consisting of at least two components into a gas chromatographic apparatus having an injection port, a column and a detection port; (b) analyzing the sample to produce a gas chromatogram; (c) providing a first data set indicative of the chromatogram to processing means; (d) applying, via the processing means, a selected number of iterations of a peak resolution function of the type employing Jansson's iterative deconvolution method to at least a selected portion of the first data set for resolving the fused peaks; and (e) providing a second data set representative of the result of the iterations of the peak resolution function; and, wherein the step of analyzing the sample to produce a gas chromatogram further comprises the steps of: (f) evaporating the same with a hot, inert gas; (g) transporting the evaporated sample from the injection port, through the column, to the detection port; and (h) detecting at least two components of the evaporated sample upon arrival at the detection port; and, wherein the step of transporting the evaporated sample further comprises the step of temporarily retaining each of the at least two components in the column.
Claim 14
Dependent Claim
80 words
Method of claim 10 wherein the step of detecting at least two components of the evaporated sample further comprises the steps of: (a) determining conductivity of the evaporated sample with respect to time; (b) determining conductivity of said inert gas with respect to time; (c) determining conductivity differences of said evaporated sample and said inert gas with respect to time; and (d) producing a waveform related to the conductivity difference detected by the detection port as a function of time.
Claim 15
Dependent Claim
24 words
Method according to claim 11 further comprising the step of plotting the second data set to provide a graphical representative of amplitude versus time.
Claim 16
Dependent Claim
56 words
Method of claim 11 wherein the peak resolution function is applied to the first data set according to: x.sup.(k+1) (t)=x.sup.(k) (t)+r[x.sup.(k) (t)](g(t)-h(t)*x.sup.(k) (t)) where: x.sup.(k) (t) is the k'th estimate of x(t); r[x.sup.(k) (t)] is the relaxation function; g(t) is the first data set; and h(t) is an impulse response function of the gas chromatographic apparatus.
Claim 17
Dependent Claim
79 words
Method of claim 13 further comprising the steps of: (a) determining a retention time for the sample; (b) selecting a pure compound having a retention time approximating the retention time of the sample; (c) injecting the pure compound into the gas chromatographic apparatus; (d) producing a impulse response chromatogram indicative of the impulse response function for the gas chromatographic apparatus; and (e) providing a impulse response function data set indicative of the impulse response chromatogram to the processing means.
Claim 18
Dependent Claim
34 words
Method of claim 11 wherein the relaxation function is determined according to: r[x.sup.(k) (t)]=b[(1-2/c) |x.sup.(k) (t)-c/2|] where: b is a relaxation constant; and c is the maximum peak height of the first data set.
Claim 19
Dependent Claim
44 words
Method of claim 11 further comprising the steps of: (a) analyzing the first data set to determine the severity of fusing of peaks of the chromatogram; and (b) selecting the number of iterations based on the severity of fusing of peaks of the chromatogram.
Claim 20
Dependent Claim
25 words
Method of claim 11 further comprising the step of preparing the first data set prior applying the peak resolution function to resolve the fused peaks.
Claim 21
Dependent Claim
43 words
Method of claim 17 wherein the step of preparing the first data set further comprises the steps of: (a) selecting a portion of the first data set for interpretation; (b) removing baseline offset from the selected portion; and (c) normalizing the selected portion.
Claim 22
Dependent Claim
44 words
Method of claim 18 wherein the selecting step further comprises the steps of: (a) determining a maximum peak value for the data set; (b) locating the maximum peak; and (c) stripping off all data from the data set not associated with the maximum peak.
Claim 23
Dependent Claim
21 words
Method of claim 18 further comprising the step of scaling the impulse response function with respect to the normalized selected portion.
Claim 24
Dependent Claim
18 words
Method of claim 18 further comprising the step of smoothing the selected portion and the impulse response function.
References Cited (Backward Citations)
US4180857A
Cited
US4215563A
Cited
US4266277A
Cited
US4357668A
Cited
US4309898A
Cited
US4353242A
Cited
US4468742A
Cited
US4403503A
Cited
US4546643A
Cited
US4740903A
Cited
US4752888A
Cited
US4741198A
Cited
US4807148A
Cited
US4802102A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-07-09 | 1994-01-08 | 1994-07-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-07-09 | 1998-01-08 | 1998-07-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-07-09 | 2002-01-08 | 2002-07-10 | $7700.00 | Window Closed / Lapsed |