US4939151A — Adherent cell culture flask

Claim 1

Independent Claim 89 words

A gas permeable cell culture flask for growing adherent cells in vitro comprising a body constructed from a gas permeable flexible film, the body having a first interior surface and a second interior surface that define a containment area, the first interior surface having a charged surface for facilitating attachment of adherent cells to the first interior surface and the second interior surface having a non-smooth surface for preventing the first interior surface from sticking to the second interior surface during a process for manufacturing and sterilizing the flask.

Claim 2

Dependent Claim 89 words

A gas permeable cell culture flask for growing adherent cells in vitro comprising a body constructed from a gas permeable flexible film, the body having a first interior surface and a second interior surface that define a containment area, the first interior surface having a charged surface for facilitating attachment of adherent cells to the first interior surface and the second interior surface having a non-smooth surface for preventing the first interior surface from sticking to the second interior surface during a process for manufacturing and sterilizing the flask.

Claim 3

Dependent Claim 17 words

The gas permeable cell culture flask of claim 1 wherein the first interior surface is negatively charged.

Claim 4

Dependent Claim 17 words

The gas permeable cell culture flask of claim 1 wherein the first interior surface is positively charged.

Claim 5

Dependent Claim 18 words

The gas permeable cell culture flask of claim 1 wherein the second interior surface has a matte texture.

Claim 6

Dependent Claim 16 words

The gas permeable cell culture flask of claim 1 wherein the second interior surface includes ribs.

Claim 7

Dependent Claim 17 words

The gas permeable cell culture flask of claim 1 wherein the body is constructed from ethylene-vinyl acetate.

Claim 8

Dependent Claim 23 words

The gas permeable cell culture flask of claim 1 wherein the flask includes at least one access port for accessing the containment area.

Claim 9

Dependent Claim 25 words

The gas permeable cell culture flask of claim 1 wherein the first interior surface has a surface energy of at least 60 dyne per centimeter.

Claim 10

Independent Claim 88 words

A method of culturing a cell line comprising the steps of: providing a container that defines a containment area; providing the container with a first interior surface that is charged; providing the container with a second interior surface, located diametric to the first interior surface, that has a non-smooth surface; introducing a cell line into the containment area; allowing the cell line to attach to the first interior surface; and allowing gases, including oxygen and carbon dioxide, t o permeate through at least one wall of the container.

Claim 11

Independent Claim 88 words

A method of culturing a cell line comprising the steps of: providing a container that defines a containment area; providing the container with a first interior surface that is charged; providing the container with a second interior surface, located diametric to the first interior surface, that has a non-smooth surface; introducing a cell line into the containment area; allowing the cell line to attach to the first interior surface; and allowing gases, including oxygen and carbon dioxide, t o permeate through at least one wall of the container.

Claim 12

Dependent Claim 18 words

The method of claim 9 including the step of providing the first interior surface with a negative charge.

Claim 13

Dependent Claim 18 words

The method of claim 9 including the step of providing the first interior surface with a positive charge.

Claim 14

Dependent Claim 26 words

The method of claim 9 including the step of providing the first interior surface with a surface of energy of at least 40 dyne per centimeter.

Claim 15

Dependent Claim 22 words

The method of claim 9 including the step of constructing the first interior surface so that it is smooth and optically clear.

Claim 16

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, wherein the interior surface of the first side wall is negatively charged, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 17

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, wherein the interior surface of the first side wall is negatively charged, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 18

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, wherein the interior surface of the first side wall is positively charged, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 19

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, wherein the interior surface of the first side wall is positively charged, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 20

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface, said means including ribs projecting from a surface of said second sidewall.

Claim 21

Dependent Claim 110 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, the second side wall being constructed from flexible film and including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface, said means including ribs projecting from a surface of said second sidewall.

Claim 22

Dependent Claim 116 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, the second side wall being constructed from flexible film, wherein the interior surfaces of both the first and second side walls are charged, said second side wall further including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 23

Dependent Claim 116 words

An adherent cell culture flask comprising a body constructed from a first side wall and a second side wall, each side wall having an interior surface and an exterior surface, the first and second side walls being sealed to define a chamber, the first side wall being constructed from flexible film and including on its interior surface means for facilitating the attachment of cells thereto, the second side wall being constructed from flexible film, wherein the interior surfaces of both the first and second side walls are charged, said second side wall further including on its interior surface means for preventing the first side wall interior surface from sticking to the second side wall interior surface.

Claim 24

Dependent Claim 30 words

The adherent cell culture flask of claim 14, 15, 16 or 17 wherein at least one of the first and second side walls is constructed from a gas permeable film.

Claim 25

Dependent Claim 24 words

The adherent cell culture flask of claim 14, 15, 16 or 17 wherein the interior surface of the second side wall is not smooth.

Claim 26

Dependent Claim 20 words

The adherent cell culture flask of claim 14, 15, 16 or 17 including an access port for accessing the chamber.

Claim 27

Dependent Claim 28 words

The adherent cell culture flask of claim 14, 15, 16 or 17 wherein at least one of the first or second side walls is constructed from ethylene-vinyl acetate.

Claim 28

Dependent Claim 26 words

The adherent cell culture flask of claim 14, 15, 16 or 17 wherein the interior surface of the first side wall is optically clear and smooth.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1993-07-02	1994-01-01	1994-07-03	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1997-07-02	1998-01-01	1998-07-03	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	2001-07-02	2002-01-01	2002-07-03	$7700.00	Window Closed / Lapsed

Adherent cell culture flask

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