EP0148161A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Cell culture chamber
Assignee: EIDP Inc
•
Filing: 1985-01-02
•
Granted: —
•
Expires: 2005-01-02
Abstract
Abstract A closed cell culture chamber of which at least one wall (14) comprises liquid impermeable, transparent ionomer film, said chamber having an access port (12) for admission of fluid thereto. The apparatus may include an edge support (32, 34) to support and tension the film.
Inventors
- 👤 Richard Terry Root
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 17:43
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/24; C12M23/46
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
36 words
A closed cell culture chamber of which at least one wall (14) comprises a sheet of a gas permeable, liquid impermeable, transparent ionomer film, said chamber having an access port (22) for admission of fluid thereto.
Claim 2
Dependent Claim
36 words
A closed cell culture chamber of which at least one wall (14) comprises a sheet of a gas permeable, liquid impermeable, transparent ionomer film, said chamber having an access port (22) for admission of fluid thereto.
Claim 3
Dependent Claim
21 words
A chamber according to claim 1 which comprises two sheets of said ionomer film, the sheets being edge sealed (12) together.
Claim 4
Dependent Claim
20 words
A chamber according to claim 1 or 2 including an edge support (32, 34) to support and tension said film.
Claim 5
Dependent Claim
30 words
A chamber according to claim 3 wherein said edge support comprises first and second frame members (32, 34) adapted to interengage each other and the edges (12) of said sheet.
Claim 6
Dependent Claim
63 words
A chamber according to any of the preceding claims in the form of a rectangular chamber having four sides, three sides being defined by an edge seal (12) between two sheets of said ionomer film, the fourth side being defined by a resin header (16) sealed to the fourth side of the sheets, the header providing access to the interior of the chamber.
Claim 7
Dependent Claim
22 words
A chamber according to any of the preceding claims wherein the ionomer film has a thickness in the range of 24-250 pm.
Claim 8
Dependent Claim
32 words
A chamber according to any of the preceding claims wherein the ionomer film has an oxygen permeability of between 3 x 10 11 and 50 x 10 -10 m / s Pa.
Claim 9
Dependent Claim
35 words
A chamber according to any of the preceding claims wherein the ionomer film has a tensile strength greater than 10.3 MPa and a puncture strength of at least about 100 as defined in ASTM 1709.
Claim 10
Dependent Claim
26 words
A chamber according to any of the preceding claims wherein the ionomer film has a haze of about 5 or less as defined in ASTM D1003.
Claim 11
Dependent Claim
75 words
A chamber according to any of claims 1 to 5 wherein the ionomer film has a thickness in the range- 48-150 µm; has an oxygen permeability of between 3 x 10- 11 and 50 x 10 -10 m/s P a; has a tensile strength greater than 10.3 MPa, a puncture strength of at least about 100 as defined in AS TM 1709; and a haze of about 5 or less as defined in ASTM D1003.
Claim 12
Dependent Claim
55 words
A chamber according to any of the preceding claims wherein said ionomer film comprises a copolymer of ethylene with 0.3-12.5 mole % (based on methacrylic acid equivalents) of acrylic acid, methacrylic acid, maleic acid or a half ester thereof, fumaric acid or a half ester thereof or itaconic acid, if desired with a further comonomer.
Claim 13
Dependent Claim
32 words
A chamber according to claim 11 wherein said further comonomer comprises up to 7.5 mole% (based on butyl acylate) of propylene, acrylate esters, methacrylate esters, vinyl esters, vinyl ethers or t-butyl ethylene.
Claim 14
Dependent Claim
25 words
A chamber according to any of the preceding claims wherein the surface of said ionomer film is chemically modified to enhance or abrogate cell adhesion.
Claim 15
Independent Claim
55 words
A method of culturing a cell line which comprises introducing a viable cell line and a suitable culture medium into the chamber defined in any of claims 1 to 13 through said access port, closing said access port, and allowing growth of said cell line to take place utilising gases permeating through said ionomer film.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.