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US4935494A
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C-terminal peptide and protein sequencing
Assignee: City of Hope
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Filing: 1988-11-15
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Granted: 1990-06-19
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Expires: 2008-11-15
Abstract
Abstract Methodology involving novel coupling reagents for the carboxyl-terminal amino acid sequence analysis of peptides and proteins is described.
Inventors
- 👤 Chad G. Miller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 01:45
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/06; C07K1/08; C07K1/082; C07K1/12; C07K1/122; C07K1/128; Y; Y10; Y10S; Y10S530/00; Y10S530/807
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
46 words
In a process for the sequential degradation of a protein or peptide, which including reacting the carboxy terminus of said protein or peptide with a coupling reagent, the improvement which comprises utilizing, as the coupling reagent, a phosphoryl(thio)amide having the Formula A, B, C or D.
Claim 2
Dependent Claim
46 words
In a process for the sequential degradation of a protein or peptide, which including reacting the carboxy terminus of said protein or peptide with a coupling reagent, the improvement which comprises utilizing, as the coupling reagent, a phosphoryl(thio)amide having the Formula A, B, C or D.
Claim 3
Dependent Claim
34 words
A process as defined by claim 1 in which the product of the reaction between said coupling agent and amino acid residue at said carboxy terminus is cleaved from the residual peptide or protein.
Claim 4
Dependent Claim
40 words
A process as defined by claim 1 or claim 2 in which the reaction of said carboxy terminus and said coupling agent is effected at a temperature of from about 25° C. to about 60° C. in an inert solvent.
Claim 5
Dependent Claim
29 words
A process as defined in claim 1 or 2 in which the reaction of said carboxy terminus and said coupling agent is effected in the presence of an amine.
Claim 6
Dependent Claim
26 words
A process as defined by claim 1 or 2 in which said peptide or protein is immobilized on a solid support prior to said coupling reaction.
References Cited (Backward Citations)
US4837165A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-18 | 1993-12-18 | 1994-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-18 | 1997-12-18 | 1998-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-18 | 2001-12-18 | 2002-06-19 | $7700.00 | Window Closed / Lapsed |