US4935407A
Expired / Lapsed
US
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Thermochemiluminescent cyclodextrin complexes
Assignee: Akzo NV
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Filing: 1987-09-22
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Granted: 1990-06-19
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Expires: 2007-09-22
Abstract
Abstract Thermochemiluminescent complexes of an adamanthylideneadamantane 1,2-dioxetane and a cyclodextrin are non-volatile at the temperatures used to excite the thermochemiluminescence. The complexes can be encapsulated in immunosensitized microcapsules, e.g., liposomes, to provide a labeled immunoreagent useful in immunoassay using thermochemiluminescent labeled immunoreagents.
Inventors
- 👤 Theo M. Luider
- 👤 Jan C. Hummelen
- 👤 Johannes N. Koek
- 👤 Hans Wynberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/58; G01N33/585; G01N33/586
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
10 words
A thermochemiluminescent complex comprising an adamantylideneadamantane 1,2-dioxetane and a cyclodextrin.
Claim 2
Dependent Claim
10 words
A thermochemiluminescent complex comprising an adamantylideneadamantane 1,2-dioxetane and a cyclodextrin.
Claim 3
Dependent Claim
12 words
A thermochemiluminescent complex according to claim 1, wherein said cyclodextrin is gamma-cyclodextrin.
Claim 4
Dependent Claim
56 words
A thermochemiluminescent complex according to claim 1, wherein said adamantylideneadamantane 1,2-dioxetane has the formula: ##STR3## wherein R 1 is selected from the group consisting of H, Cl, Br, F, I, OH, NH 2 , OR 2 and NHR 2 , and wherein R 2 is an aliphatic organic radical containing no more than 20 carbon atoms.
Claim 5
Dependent Claim
15 words
A thermochemiluminescent complex according to claim 1, wherein said adamantylideneadamantane 1,2-dioxetane has the formula: ##STR4##
Claim 6
Dependent Claim
12 words
A thermochemiluminescent complex according to claim 4, wherein said cyclodextrin is gamma-cyclodextrin.
Claim 7
Independent Claim
91 words
A method of immunoanalysis comprising encapsulating an aqueous solution comprising a thermochemiluminescent complex of an adamantylideneadamantane 1,2-dioxetane and a cyclodextrin within a microcapsule having an exterior surface, said exterior surface bearing an immunoreagent, contacting said microcapsule in aqueous suspension with an immunoreagent complementary to the immunoreagent on said exterior surface, whereby an immunocomplex is formed, separating said immunocomplex from said aqueous suspension, heating said separated immunocomplex to a temperature at which said complex undergoes a thermochemiluminescent reaction thereby emitting light, and determining the amount of light emitted in said thermochemiluminescent reaction.
Claim 8
Independent Claim
91 words
A method of immunoanalysis comprising encapsulating an aqueous solution comprising a thermochemiluminescent complex of an adamantylideneadamantane 1,2-dioxetane and a cyclodextrin within a microcapsule having an exterior surface, said exterior surface bearing an immunoreagent, contacting said microcapsule in aqueous suspension with an immunoreagent complementary to the immunoreagent on said exterior surface, whereby an immunocomplex is formed, separating said immunocomplex from said aqueous suspension, heating said separated immunocomplex to a temperature at which said complex undergoes a thermochemiluminescent reaction thereby emitting light, and determining the amount of light emitted in said thermochemiluminescent reaction.
Claim 9
Dependent Claim
12 words
The method according to claim 6, wherein said cyclodextrin is a gamma-cyclodextrin.
Claim 10
Dependent Claim
56 words
The method according to claim 6, wherein said thermochemiluminescent adamantylideneadamantane 1,2-dioxetane has the formula: ##STR5## wherein R 1 is selected from the group consisting of H, Cl, Br, F, I, OH, NH 2 , OR 2 and NHR 2 , and wherein R 2 is an aliphatic organic radical containing no more than 20 carbon atoms.
Claim 11
Dependent Claim
15 words
The method according to claim 6, wherein said thermochemiluminescent adamantylideneadamantane 1,2-dioxetane has the formula: ##STR6##
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-06-18 | 1993-12-18 | 1994-06-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-06-18 | 1997-12-18 | 1998-06-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-06-18 | 2001-12-18 | 2002-06-19 | $7700.00 | Window Closed / Lapsed |