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US4929545A
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Method and reagent for determination of an analyte via enzymatic means using a ferricyanide/ferric compound system
Assignee: Roche Diagnostics Corp
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Filing: 1989-04-14
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Granted: 1990-05-29
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Expires: 2009-04-14
Abstract
Abstract A composition useful in determining analytes, such as glucose or cholesterol, is disclosed. The composition contains an enzyme which specifically reacts with the analyte to be determined, a soluble ferricyanide compound which is reducible to form a ferrocyanide, a soluble ferric compound which provides ferric ions for reacting with ferrocyanide to form a reaction product, and a buffer which does not prevent formation of this reaction product. The composition has a pH of from about 3.0 to about 6.0.
Inventors
- 👤 Helmut Freitag
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 13:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
81 words
Composition useful in determining an analyte in a sample, comprising: (a) an enzyme which specifically reacts with said analyte; (b) a soluble ferricyanide compound which is reducible in the presence of an electron to produce a ferrocyanide compound; (c) a soluble ferric compound which reacts with a ferrocyanide compound to form a reaction product therebetween, and (d) a buffer which does not prevent formation of said reaction product, wherein said composition has a pH of from about 3.0 to about 6.0.
Claim 2
Dependent Claim
81 words
Composition useful in determining an analyte in a sample, comprising: (a) an enzyme which specifically reacts with said analyte; (b) a soluble ferricyanide compound which is reducible in the presence of an electron to produce a ferrocyanide compound; (c) a soluble ferric compound which reacts with a ferrocyanide compound to form a reaction product therebetween, and (d) a buffer which does not prevent formation of said reaction product, wherein said composition has a pH of from about 3.0 to about 6.0.
Claim 3
Dependent Claim
10 words
Composition of claim 1, wherein said enzyme is glucose oxidase.
Claim 4
Dependent Claim
10 words
Composition of claim 1, wherein said enzyme is cholesterol oxidase.
Claim 5
Dependent Claim
11 words
Composition of claim 1, wherein said buffer is 4-amino butyric acid.
Claim 6
Dependent Claim
17 words
Composition of claim 1, wherein said composition has a pH of from about 3.9 to about 4.6.
Claim 7
Dependent Claim
17 words
Composition of claim 1, wherein said composition has a pH of from about 3.9 to about 4.2.
Claim 8
Dependent Claim
15 words
Composition of claim 1, in the form of a solution which further comprises distilled water.
Claim 9
Dependent Claim
10 words
Composition of claim 1, in the form of a powder.
Claim 10
Dependent Claim
12 words
Composition of claim 1, wherein said soluble ferricyanide compound is potassium ferricyanide.
Claim 11
Dependent Claim
15 words
Composition of claim 1, wherein said soluble ferric compound is ferric chloride or ferric sulfate.
Claim 12
Dependent Claim
8 words
Composition of claim 1, impregnated onto a carrier.
Claim 13
Dependent Claim
7 words
Composition of claim 1, in lyophilized form.
Claim 14
Dependent Claim
38 words
Composition of claim 1 in the form of a kit comprising separate containers of each of said enzyme, said soluble ferricyanide compound, said soluble ferric compound and said buffer, and a container means for holding said separate containers.
Claim 15
Dependent Claim
39 words
Method for determining an analyte in a sample comprising a liquid sample containing an analyte which reacts with the enzyme of the composition of claim 1 and determining formation of said reaction product as a measure of said analyte.
Claim 16
Dependent Claim
12 words
Method of claim 14, wherein said sample is a body fluid sample.
Claim 17
Dependent Claim
9 words
Method of claim 14, wherein said analyte is glucose.
Claim 18
Dependent Claim
9 words
Method of claim 14, wherein said analyte is cholesterol.
Claim 19
Dependent Claim
56 words
Method for determining presence of an analyte in a liquid sample comprising contacting a liquid sample to the composition of claim 1, wherein said composition contains an enzyme which reacts with said analyte and determining formation of said reaction product, wherein formation of said reaction product is indicative of presence of said analyte in said sample.
Claim 20
Dependent Claim
102 words
Analytical element useful in determining an analyte in a sample, comprising: (a) a support carrier; (b) a reagent layer applied to said support carrier, said reagent layer comprising (i) an inert film, (ii) an enzyme which specifically reacts with said analyte, (iii) a soluble ferricyanide compound which is reduced in the presence of an electron to a ferrocyanide compound, (iv) a soluble ferric compound which reacts with said ferrocyanide compound to form a reaction product, and (v) a buffer which does not prevent formation of said reaction product, wherein said reagent layer has a pH of from about 3.0 to about 6.0.
Claim 21
Dependent Claim
102 words
Analytical element useful in determining an analyte in a sample, comprising: (a) a support carrier; (b) a reagent layer applied to said support carrier, said reagent layer comprising (i) an inert film, (ii) an enzyme which specifically reacts with said analyte, (iii) a soluble ferricyanide compound which is reduced in the presence of an electron to a ferrocyanide compound, (iv) a soluble ferric compound which reacts with said ferrocyanide compound to form a reaction product, and (v) a buffer which does not prevent formation of said reaction product, wherein said reagent layer has a pH of from about 3.0 to about 6.0.
Claim 22
Dependent Claim
10 words
Analytical element of claim 19, further comprising a nonionic detergent.
Claim 23
Dependent Claim
10 words
Analytical element of claim 19, further comprising an inert pigment.
Claim 24
Dependent Claim
15 words
Analytical element of claim 19, having a pH of from about 3.9 to about 4.6.
Claim 25
Dependent Claim
15 words
Analytical element of claim 19, having a pH of from about 3.9 to about 4.2.
References Cited (Backward Citations)
US4576913A
Cited
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